Human CD200 enzyme-linked immunoassay kit

見積もりを依頼する

お問い合わせ

E-mail: [email protected]

TEL: 0571-88062880

代理店・販売店を探す

Catalog# EH0173

Human CD200 enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

31.25~2000 pg/ml

製品概要

製品名

Human CD200 enzyme-linked immunoassay kit

製品タイプ

Elisa Kit

検出感度

0.55 pg/ml (50 μl)；4.24 pg/ml (10 μl)；

pg/ml	O.D.		Average	Corrected
0.00	0.0197	0.0199	0.0198
31.25	0.0989	0.0996	0.0993	0.0795
62.50	0.1858	0.1874	0.1866	0.1668
125.00	0.3507	0.3609	0.3558	0.3360
250.00	0.7370	0.6479	0.6925	0.6727
500.00	1.3060	1.2280	1.2670	1.2472
1000.00	2.3000	2.1670	2.2335	2.2137
2000.00	3.7050	3.6340	3.6695	3.6497

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	47.5	227.2	648.6	45.6	220.5	665.2
Standard Deviation	2.2	6.8	22.6	2.1	10.0	19.0
Coefficient of Variation (%)	4.6	3.0	3.5	4.5	4.5	2.9

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of human CD200 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 110% with an overall mean recovery of 98%.

サンプル値

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	187.6-436.31	100	315.16

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CD200 in this assay. No medical histories were available for the donors.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

120min

標準曲線梯度

7点

インキュベーション回数

Detectable Sample

可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。

サンプル添加量

50 μl/10 μl

製品の特徴

保存条件

2-8 ℃で保存

ターゲット

UNIPROT #

SwissProt: P41217 Human

別名

Antigen identified by monoclonal MRC OX 2

CD200

CD200 antigen

CD200 molecule

MOX1

MOX2

MRC

MRC OX 2 antigen

My033

OX 2

展開

機能

CD200, also known as OX-2, is a 45 kDa transmembrane immunoregulatory protein that belongs to the immunoglobulin superfamily. The human CD200 cDNA encodes a 278 amino acid (aa) precursor that includes a 30 aa signal sequence, a 202 aa extracellular domain (ECD), a 27 aa transmembrane segment, and a 19 aa cytoplasmic domain. The ECD is composed of one Ig-like V-type domain and one Ig-like C2-type domain. A splice variant of CD200 has been described and has a truncated cytoplasmic tail. Within the ECD, human CD200 shares 76% aa sequence identity with mouse and rat CD200. CD200 is widely but not ubiquitously expressed. Its receptor (CD200R) is restricted primarily to mast cells, basophils, macrophages, and dendritic cells, which suggests myeloid cell regulation as the major function of CD200. CD200 knockout mice are characterized by increased macrophage number and activation and are predisposed to autoimmune disorders. CD200 and CD200R associate via their respective N-terminal Ig-like domains. In myeloid cells, CD200R initiates inhibitory signals following receptor‑ligand contact. In T cells, however, CD200 functions as a costimulatory molecule independent of the CD28 pathway. Several additional CD200R-like molecules have been identified in human and mouse, but their capacity to interact with CD200 is controversial. Several viruses encode CD200 homologs which are expressed on infected cells during the lytic phase. Like CD200 itself, viral CD200 homologs also suppress myeloid cell activity, enabling increased viral propagation.

Human CD200 enzyme-linked immunoassay kit

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

ターゲット

UNIPROT #

別名

機能

同一ターゲット・同一パスウェイ製品

Human CD47 enzyme-linked immunoassay kit

Human beta Amyloid 1-42 enzyme-linked immunoassay kit

Human/Mouse MASP2 enzyme-linked immunoassay kit

Human SAA1 enzyme-linked immunoassay kit

Human CD254/RANKL/TNFSF11 enzyme-linked immunoassay kit

Human IL-3 enzyme-linked immunoassay kit

Human Complement factor B enzyme-linked immunoassay kit(one step)

Human IL-17E/IL-25 enzyme-linked immunoassay kit

Human Myeloperoxidase(MPO) enzyme-linked immunoassay kit(one step)

Human Leptin enzyme-linked immunoassay kit

Human PAI-1/Serpin E1 enzyme-linked immunoassay kit(one step)

Human ULBP1/NKG2DL1 enzyme-linked immunoassay kit

Human CD273/B7-DC/PD-L2 enzyme-linked immunoassay kit（one step）

Human VE Cadherin enzyme-linked immunoassay kit

Human CD62E enzyme-linked immunoassay kit

Human/Monkey alpha Fetoprotein enzyme-linked immunoassay kit

Human CX3CL1/Fractalking enzyme-linked immunoassay kit

Human/Monkey PCSK9 enzyme-linked immunoassay kit (one step)

Human Growth Hormone enzyme-linked immunoassay kit

Human Nectin-2 enzyme-linked immunoassay kit

Human/Monkey/Canine Osteopontin（OPN） enzyme-linked immunoassay kit

Human Total HSP60 enzyme-linked immunoassay kit

Human Angiotensinogen enzyme-linked immunoassay kit

Human IL-33 enzyme-linked immunoassay kit

Human RAGE enzyme-linked immunoassay kit（one step）

Human Prostate specific Antigen/KLK3 enzyme-linked immunoassay kit

Human CD48 enzyme-linked immunoassay kit

Human/Monkey Albumin enzyme-linked immunoassay kit(one step)

Human IL-13 enzyme-linked immunoassay kit

Human B7-H2/CD275 enzyme-linked immunoassay kit

Human Alpha-Synuclein enzyme-linked immunoassay kit

Human MMP-3 enzyme-linked immunoassay kit

Human MERTK enzyme-linked immunoassay kit（one step）

Human Adiponectinenzyme-linked immunoassay kit（one step）

Human CCL25 enzyme-linked immunoassay kit

Human Lipoprotein A/LPA enzyme-linked immunoassay kit(one step)

Human TNF RI/TNFRSF1A enzyme-linked immunoassay kit

Human TREM-1 enzyme-linked immunoassay kit

Human Lipoprotein A/LPA enzyme-linked immunoassay kit

Human tPA enzyme-linked immunoassay kit(one step)

Human MICB enzyme-linked immunoassay kit

Human Antithrombin III/AT III enzyme-linked immunoassay kit(one step)

Human TSLP enzyme-linked immunoassay kit

Human/Monkey Clusterin enzyme-linked immunoassay kit(one step)

Human SCF/KITLG enzyme-linked immunoassay kit

Human CD33 enzyme-linked immunoassay kit

Human CXCL5/ENA-78 enzyme-linked immunoassay kit

Human/Monkey/Canine CXCL10/IP-10 enzyme-linked immunoassay kit

Human CHIT1 enzyme-linked immunoassay kit

Human CCL17/TARC enzyme-linked immunoassay kit

Human/Monkey/Canine IL-18 enzyme-linked immunoassay kit

Human FGF-19 enzyme-linked immunoassay kit

Human/Monkey Complement C3 enzyme-linked immunoassay kit(one step)

Human IL-6 Super Sensitivity enzyme-linked immunoassay kit

Human Oxytocin-neurophysin 1/OXT enzyme-linked immunoassay kit

Human Proprotein Convertase 9/PCSK9 enzyme-linked immunoassay kit（one step）