Human NKp46/NCR1 enzyme-linked immunoassay kit

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Catalog# EH0221

Human NKp46/NCR1 enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

1.37~1000 pg/ml

製品概要

製品名

Human NKp46/NCR1 enzyme-linked immunoassay kit

製品タイプ

Elisa Kit

検出感度

0.06 pg/ml (50 μl)；1.09 pg/ml (10 μl)

pg/ml	O.D.		Average	Corrected
0.00	0.0119	0.0135	0.0127
1.37	0.0232	0.0240	0.0236	0.0232
4.12	0.0501	0.0495	0.0498	0.0501
12.35	0.1274	0.1232	0.1253	0.1274
37.04	0.3634	0.3362	0.3498	0.3634
111.11	0.9253	0.9344	0.9298	0.9253
333.33	2.2430	2.0810	2.1620	2.2430
1000.00	3.7100	3.7730	3.7415	3.7100

精度

	Intra-Assay Precision			Inter-Assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	30.2	162.4	401.4	22.2	134.6	462.6
Standard Deviation	1.1	4.7	6.9	1.3	4.8	13.7
Coefficient of Variation (%)	3.7	2.9	1.7	5.6	3.5	3.0

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

スパイク回収

The spike recovery was evaluated by spiking 3 levels of human NKp46/NCR1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 86% to 109% with an overall mean recovery of 95%.

サンプル値

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
serum	30	115.20-331.30	100	218.98

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of NKp46/NCR1 in this assay. No medical histories were available for the donors.

n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.

実験タイプ

Sandwich Elisa

種属反応性

Human

検出方法

Colorimetric

所要時間

120 min

標準曲線梯度

7点/3倍

インキュベーション回数

Detectable Sample

可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。

サンプル添加量

10 μl/50 μl

製品の特徴

保存条件

2-8 ℃で保存

ターゲット

UNIPROT #

SwissProt: O76036 Human

機能

NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR). These receptors are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells. In human systems, the CD56dimCD16+ subpopulation that makes up the majority of NK cells in the peripheral blood and spleen expresses NKp46 in both resting and activated states. The main NK cell population of the lymph node (CD56brightCD16-) expresses low levels of NKp46 in resting cells, but expression is upregulated by IL-2. Mouse NKp46, also known as MAR-1, is a type I transmembrane protein with two extracellular Ig-like domains. It has a positive charge in its transmembrane domain that permits association with the ITAM-bearing signal adapter proteins, CD3 zeta and Fc epsilon RI gamma. Studies with neutralizing antibodies indicate that the three NCR are primarily responsible for triggering the NK-mediated lysis of many human tumor cell lines. Blocking any of the NCRs individually resulted in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis was observed if all three receptors were blocked simultaneously. NKp46 has also been implicated in recognition of virus-infected cells through its capacity to bind to viral hemagglutinins.

Human NKp46/NCR1 enzyme-linked immunoassay kit

お問い合わせ

製品概要

製品名

製品タイプ

検出感度

精度

スパイク回収

サンプル値

実験タイプ

種属反応性

検出方法

所要時間

標準曲線梯度

インキュベーション回数

Detectable Sample

サンプル添加量

製品の特徴

保存条件

ターゲット

UNIPROT #

機能

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