Human/Mouse MASP2 enzyme-linked immunoassay kit
Catalog# EH0231
Human/Mouse MASP2 enzyme-linked immunoassay kit
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ELISA
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Human
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156.25~10000 pg/ml
製品概要
製品名
Human/Mouse MASP2 enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
0.65 pg/ml (50 μl)；14.43 pg/ml (10 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0088 | 0.0087 | 0.0088 | |
| 156.25 | 0.1134 | 0.1133 | 0.1134 | 0.1046 |
| 312.50 | 0.2231 | 0.2098 | 0.2165 | 0.2077 |
| 625.00 | 0.4160 | 0.4043 | 0.4102 | 0.4014 |
| 1250.00 | 0.7754 | 0.7621 | 0.7688 | 0.7600 |
| 2500.00 | 1.4450 | 1.3370 | 1.3910 | 1.3823 |
| 5000.00 | 2.3410 | 2.3980 | 2.3695 | 2.3608 |
| 10000.00 | 3.4990 | 3.5180 | 3.5085 | 3.4998 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 201.2 | 968.9 | 3123.1 | 197.1 | 1011.0 | 3287.9 |
| Standard Deviation | 9.5 | 46.0 | 104.7 | 8.3 | 47.6 | 158.0 |
| Coefficient of Variation (%) | 4.7 | 4.7 | 3.4 | 4.2 | 4.7 | 4.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human/mouse MASP2 into health human/mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 120% with an overall mean recovery of 101%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 12.58-41.69 | 100 | 23.31 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of MASP2 in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
Mannan binding lectin serine peptidase 2
Mannan binding lectin serine protease 2
Mannose binding protein associated serine protease 2
MAP 19
MAP19
MASP 2
MBL associated plasma protein of 19 kD
MBL associated serine protease 2
Small MBL associated protein
sMAP