Mouse RANKL/TRANCE/TNFSF11 enzyme-linked immunoassay kit
Catalog# EM0044
Mouse RANKL/TRANCE/TNFSF11 enzyme-linked immunoassay kit
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ELISA
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Mouse
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2.74~2000 pg/ml
製品概要
製品名
Mouse RANKL/TRANCE/TNFSF11 enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
0.36 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0138 | 0.0142 | 0.0140 | |
| 2.74 | 0.0234 | 0.0247 | 0.0241 | 0.0101 |
| 8.23 | 0.0475 | 0.0485 | 0.0480 | 0.0340 |
| 24.69 | 0.1120 | 0.1176 | 0.1148 | 0.1008 |
| 74.07 | 0.3230 | 0.3205 | 0.3218 | 0.3078 |
| 222.22 | 0.9004 | 0.8719 | 0.8862 | 0.8722 |
| 666.67 | 2.2855 | 2.2587 | 2.2721 | 2.2581 |
| 2000.00 | 4.1051 | 3.9973 | 4.0512 | 4.0372 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 60.20 | 211.59 | 633.72 | 62.06 | 191.99 | 568.45 |
| Standard Deviation | 3.50 | 8.89 | 30.71 | 3.23 | 10.65 | 28.61 |
| Coefficient of Variation (%) | 5.8 | 4.2 | 4.8 | 5.2 | 5.5 | 5.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested Twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of mouse RANKL/TRANCE/TNFSF11 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 90% to 111% with an overall mean recovery of 100%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 61.89-141.84 | 100 | 107.38 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of RANKL/TRANCE/TNFSF11 in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
CD254
hRANKL2
ODF
OPGL
OPTB2
Osteoclast differentiation factor
Osteoprotegerin ligand
Rank Ligand
RANKL
Receptor activator of nuclear factor kappa B ligand
展開