Mouse Insulin Super Sensitivity enzyme-linked immunoassay kit
Catalog# EMSS0067
Mouse Insulin Super Sensitivity enzyme-linked immunoassay kit
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ELISA
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Mouse
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15.63~1000 pg/ml
製品概要
製品名
Mouse Insulin Super Sensitivity enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
14.98 pg/ml (20 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0483 | 0.0483 | 0.0483 | |
| 15.63 | 0.0628 | 0.0628 | 0.0628 | 0.0145 |
| 31.25 | 0.0710 | 0.0710 | 0.0710 | 0.0227 |
| 62.50 | 0.0966 | 0.0966 | 0.0966 | 0.0483 |
| 125.00 | 0.1699 | 0.1699 | 0.1699 | 0.1216 |
| 250.00 | 0.3775 | 0.3775 | 0.3775 | 0.3292 |
| 500.00 | 1.1460 | 1.1660 | 1.1560 | 1.1077 |
| 1000.00 | 3.1760 | 3.1760 | 3.1760 | 3.1277 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 581.0 | 2316.1 | 7446.9 | 579.8 | 2277.0 | 7736.2 |
| Standard Deviation | 12.8 | 34.2 | 243.7 | 14.4 | 34.5 | 230.5 |
| Coefficient of Variation (%) | 2.2 | 1.5 | 3.3 | 2.5 | 1.5 | 3.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of Mouse Insulin into health serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 120% with an overall mean recovery of 110%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-1144.00 | 93.3 | 368.21 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of Insulin in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存