Mouse Coagulation Factor XII enzyme-linked immunoassay kit(one step)
Catalog# EMY0001
Mouse Coagulation Factor XII enzyme-linked immunoassay kit(one step)
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ELISA
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Mouse
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4.69~300 ng/ml
製品概要
製品名
Mouse Coagulation Factor XII enzyme-linked immunoassay kit(one step)
製品タイプ
Elisa Kit
検出感度
0.22 ng/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0060 | 0.0054 | 0.0057 | |
| 4.69 | 0.0766 | 0.0693 | 0.0730 | 0.0673 |
| 9.38 | 0.1558 | 0.1445 | 0.1502 | 0.1445 |
| 18.75 | 0.2989 | 0.3013 | 0.3001 | 0.2944 |
| 37.50 | 0.6302 | 0.5610 | 0.5956 | 0.5899 |
| 75.00 | 1.2021 | 1.2191 | 1.2106 | 1.2049 |
| 150.00 | 2.4639 | 2.3883 | 2.4261 | 2.4204 |
| 300.00 | 4.0653 | 4.0355 | 4.0504 | 4.0447 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 5.6 | 30.7 | 103.6 | 4.3 | 25.4 | 85.7 |
| Standard Deviation | 0.2 | 1.3 | 6.9 | 0.2 | 1.7 | 3.4 |
| Coefficient of Variation (%) | 3.0 | 4.2 | 6.7 | 5.5 | 6.6 | 4.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested Twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of Mouse Coagulation Factor XII into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 93% to 106% with an overall mean recovery of 100%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 29.16-54.27 | 100.0 | 42.19 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of Coagulation Factor XII in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
Factor XII
Beta factor XIIa part 1
Beta factor XIIa part 2
Coagulation factor XII
Coagulation factor XIIa heavy chain
Coagulation factor XIIa light chain
F12
F12 deficiency
FA12_HUMAN
Factor XII deficiency
展開