Mouse IL-16 enzyme-linked immunoassay kit
Catalog# EM0070
Mouse IL-16 enzyme-linked immunoassay kit
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ELISA
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Mouse
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12.29~3000 pg/ml
製品概要
製品名
Mouse IL-16 enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
2.43 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0275 | 0.0264 | 0.0270 | |
| 12.29 | 0.0387 | 0.0434 | 0.0411 | 0.0141 |
| 30.72 | 0.0524 | 0.0630 | 0.0577 | 0.0308 |
| 76.80 | 0.0947 | 0.1025 | 0.0986 | 0.0717 |
| 192.00 | 0.1932 | 0.2175 | 0.2054 | 0.1784 |
| 480.00 | 0.4853 | 0.5141 | 0.4997 | 0.4728 |
| 1200.00 | 1.2560 | 1.3040 | 1.2800 | 1.2531 |
| 3000.00 | 3.1270 | 2.9260 | 3.0265 | 2.9996 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 1002.69 | 371.96 | 79.16 | 960.9 | 340.3 | 72.3 |
| Standard Deviation | 65.41 | 23.29 | 5.45 | 47.0 | 19.0 | 5.7 |
| Coefficient of Variation (%) | 6.5 | 6.3 | 6.9 | 4.9 | 5.6 | 7.9 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of mouse IL-16 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 119% with an overall mean recovery of 108%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 259.31-1393.01 | 100 | 505.88 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存