Mouse Pro-collagen I alpha 1/COL1A1 enzyme-linked immunoassay kit（one step）
Catalog# EMY0045
Mouse Pro-collagen I alpha 1/COL1A1 enzyme-linked immunoassay kit（one step）
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ELISA
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Mouse
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0.27~200 ng/ml
製品概要
製品名
Mouse Pro-collagen I alpha 1/COL1A1 enzyme-linked immunoassay kit（one step）
製品タイプ
Elisa Kit
検出感度
0.01 pg/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0059 | 0.0063 | 0.0061 | |
| 0.27 | 0.0196 | 0.0204 | 0.0200 | 0.0139 |
| 0.82 | 0.0474 | 0.0530 | 0.0502 | 0.0441 |
| 2.47 | 0.1412 | 0.1520 | 0.1466 | 0.1405 |
| 7.41 | 0.4025 | 0.4208 | 0.4117 | 0.4056 |
| 22.22 | 1.1140 | 1.2170 | 1.1655 | 1.1594 |
| 66.67 | 2.8660 | 2.8730 | 2.8695 | 2.8634 |
| 200.00 | 4.1954 | 4.3502 | 4.2728 | 4.2667 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 5.4 | 26.4 | 74.1 | 4.0 | 21.4 | 79.3 |
| Standard Deviation | 0.4 | 2.1 | 4.3 | 0.2 | 0.4 | 4.1 |
| Coefficient of Variation (%) | 6.7 | 7.8 | 5.8 | 4.8 | 2.0 | 5.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested Twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of Mouse Pro-collagen I alpha 1/COL1A1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 86% to 119% with an overall mean recovery of 103%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 146.01-320.89 | 100 | 223.31 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of Pro-collagen I alpha 1/COL1A1 in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
Alpha 1 type I collagen
alpha1(I) procollagen
CO1A1_HUMAN
COL1A1
collagen alpha 1 chain type I
Collagen alpha-1(I) chain
collagen alpha-1(I) chain preproprotein
Collagen I alpha 1 polypeptide
collagen of skin, tendon and bone, alpha-1 chain
Collagen type I alpha 1
展開