Mouse IL-1 alpha/IL-1 F1 enzyme-linked immunoassay kit
Catalog# EM0047
Mouse IL-1 alpha/IL-1 F1 enzyme-linked immunoassay kit
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ELISA
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Mouse
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1.37~1000 pg/ml
製品概要
製品名
Mouse IL-1 alpha/IL-1 F1 enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
0.05 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0080 | 0.0069 | 0.0075 | |
| 1.37 | 0.0276 | 0.0258 | 0.0267 | 0.0193 |
| 4.12 | 0.0674 | 0.0614 | 0.0644 | 0.0570 |
| 12.35 | 0.1841 | 0.1823 | 0.1832 | 0.1758 |
| 37.04 | 0.5320 | 0.5206 | 0.5263 | 0.5189 |
| 111.11 | 1.4580 | 1.4540 | 1.4560 | 1.4486 |
| 333.33 | 3.0850 | 2.9580 | 3.0215 | 3.0141 |
| 1000.00 | 4.2378 | 4.2092 | 4.2235 | 4.2161 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 31.84 | 78.30 | 274.99 | 35.74 | 90.87 | 293.41 |
| Standard Deviation | 1.70 | 3.62 | 11.32 | 1.21 | 3.02 | 13.13 |
| Coefficient of Variation (%) | 5.4 | 4.6 | 4.1 | 3.4 | 3.3 | 4.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested Twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of mouse IL-1 alpha/IL-1F1 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 88% to 105% with an overall mean recovery of 99%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 4.98-28.97 | 100 | 15.61 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of IL-1 alpha/IL-1F1 in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存