Mouse Apolipoprotein E enzyme-linked immunoassay kit(one step)
Catalog# EMY0065
Mouse Apolipoprotein E enzyme-linked immunoassay kit(one step)
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ELISA
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Mouse
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8.23~6000 ng/ml
製品概要
製品名
Mouse Apolipoprotein E enzyme-linked immunoassay kit(one step)
製品タイプ
Elisa Kit
検出感度
0.01 ng/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0082 | 0.0093 | 0.0088 | |
| 8.23 | 0.0197 | 0.0188 | 0.0193 | 0.0105 |
| 24.69 | 0.0428 | 0.0384 | 0.0406 | 0.0319 |
| 74.07 | 0.1169 | 0.1058 | 0.1114 | 0.1026 |
| 222.22 | 0.3197 | 0.3156 | 0.3177 | 0.3089 |
| 666.67 | 0.8464 | 0.7401 | 0.7933 | 0.7845 |
| 2000.00 | 1.8530 | 1.7340 | 1.7935 | 1.7848 |
| 6000.00 | 3.2390 | 3.2740 | 3.2565 | 3.2478 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 99.7 | 550.6 | 1849.3 | 97.7 | 532.6 | 1867.9 |
| Standard Deviation | 4.7 | 25.2 | 76.2 | 3.3 | 17.8 | 82.8 |
| Coefficient of Variation (%) | 4.8 | 4.6 | 4.1 | 3.4 | 3.4 | 4.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of mouse Apolipoprotein E into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 101% with an overall mean recovery of 89%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum | 30 | 50.46-195.77 | 100 | 123.46 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated in this assay. No medical histories were available for the donors.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存