HRP Conjugated Histone H3 Recombinant Rabbit Monoclonal Antibody [JJ090-07] - Loading control

製品仕様

100μl

200μl

1ml

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Catalog# HA724276H

HRP Conjugated Histone H3 Recombinant Rabbit Monoclonal Antibody [JJ090-07] - Loading control

Application

Reactivity

Human
Mouse
Rat
Monkey

Conjugation

概要

製品名

HRP Conjugated Histone H3 Recombinant Rabbit Monoclonal Antibody [JJ090-07] - Loading control

抗体の種類

Recombinant Rabbit monoclonal Antibody

免疫原

Recombinant protein within human Histone H3 aa 85-136/136.

種属反応性

Human, Mouse, Rat, Monkey

検証された応用例

分子量

Predicted band size: 15 kDa

陽性対照

HeLa cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, PC-12 cell lysate, COS-1 cell lysate.

結合

HRP

クローン番号

JJ090-07

製品の特徴

形態

Liquid

保存方法

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

保存用バッファー

PBS (pH7.4), 0.1% BSA, 40% Glycerol.

アイソタイプ

IgG

精製方法

Protein A affinity purified.

応用希釈度

WB
1:5,000-1:20,000

ターゲット

機能

In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of histones H2A, H2B, H3 and H4, and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. p300 preferentially acetylates Histone H3 at lysines 14 and 18 and Histone H4 at lysines 5 and 8. PCAF in its native form, primarily acetylates Histone H3 at lysine 14 to a monoacetylated form, and less efficiently acetylates Histone H4 at lysine 8. Histone H4 may also be acetylated at lysines 12 and 16, and the involvement of acetylated H4 with Histones H2A, H2B and H3 suggests that acetylated histones may be involved in dynamic chromatin remodeling.

背景文献

1. Wani S et al. Human SCP4 is a chromatin-associated CTD phosphatase and exhibits the dynamic translocation during erythroid differentiation. J Biochem 160:111-20 (2016).

2. Ni JZ et al. A transgenerational role of the germline nuclear RNAi pathway in repressing heat stress-induced transcriptional activation in C. elegans. Epigenetics Chromatin 9:3 (2016).

亜細胞局在

Nucleus, Chromosome.

UNIPROT #

SwissProt: P68431 Human

SwissProt: P84243 Human

SwissProt: Q16695 Human

SwissProt: Q6NXT2 Human

SwissProt: Q71DI3 Human

SwissProt: P68433 Mouse

SwissProt: P84228 Mouse

SwissProt: Q6LED0 Rat

別名

H3 histone family, member A antibody

H3/A antibody

H31_HUMAN antibody

H3FA antibody

Hist1h3a antibody

HIST1H3B antibody

HIST1H3C antibody

HIST1H3D antibody

HIST1H3E antibody

HIST1H3F antibody

展開

画像

Western blot analysis of Histone H3 on different lysates with Mouse anti-Histone H3 antibody (HA724276H) at 1/10,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: RAW264.7 cell lysate
Lane 5: C6 cell lysate
Lane 6: PC-12 cell lysate
Lane 7: COS-1 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 15 kDa
Observed band size: 15 kDa

Exposure time: 10 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724276H, HRP) at 1/10,000 dilution was used in primary antibody dilution buffer (K1803) at room temperature for 1 hour.

ご注意ください: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

HRP Conjugated Histone H3 Recombinant Rabbit Monoclonal Antibody [JJ090-07] - Loading control

製品仕様

お問い合わせ

概要

製品名

抗体の種類

免疫原

種属反応性

検証された応用例

分子量

陽性対照

結合

クローン番号

製品の特徴

形態

保存方法

保存用バッファー

アイソタイプ

精製方法

応用希釈度

ターゲット

機能

背景文献

亜細胞局在

UNIPROT #

別名

画像

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