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Histone H3
HRP Conjugated Histone H3 Recombinant Rabbit Monoclonal Antibody [JJ090-07] - Loading control

HRP Conjugated Histone H3 Recombinant Rabbit Monoclonal Antibody [JJ090-07] - Loading control

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MSDS
COA

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Catalog# HA724276H

HRP Conjugated Histone H3 Recombinant Rabbit Monoclonal Antibody [JJ090-07] - Loading control

Application

Reactivity

Human
Mouse
Rat
Green monkey

Conjugation

Safety datasheet

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MSDS_HUABIO.pdf
MSDS_HUABIO.pdf
No MSDS Found

製品概要

製品名

HRP Conjugated Histone H3 Recombinant Rabbit Monoclonal Antibody [JJ090-07] - Loading control

抗体のタイプ

Recombinant Rabbit monoclonal Antibody

免疫原

Recombinant protein within human Histone H3 aa 85-136/136.

交差反応性（対応種属）

Human, Mouse, Rat, Green monkey

検証済みアプリケーション

標的分子量

Predicted band size: 15 kDa

ポジティブコントロール

HeLa cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, PC-12 cell lysate, COS-1 cell lysate.

コンジュゲーション

HRP

クローン番号

JJ090-07

製品の特徴

形態

Liquid

濃度

ロット濃度照会

保存方法

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

保存バッファー

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

アイソタイプ

IgG

精製方法

Protein A affinity purified.

推奨希釈倍率

WB
1:5,000-1:20,000

ターゲット

機能

In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of histones H2A, H2B, H3 and H4, and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. p300 preferentially acetylates Histone H3 at lysines 14 and 18 and Histone H4 at lysines 5 and 8. PCAF in its native form, primarily acetylates Histone H3 at lysine 14 to a monoacetylated form, and less efficiently acetylates Histone H4 at lysine 8. Histone H4 may also be acetylated at lysines 12 and 16, and the involvement of acetylated H4 with Histones H2A, H2B and H3 suggests that acetylated histones may be involved in dynamic chromatin remodeling.

背景・参考文献

1. Wani S et al. Human SCP4 is a chromatin-associated CTD phosphatase and exhibits the dynamic translocation during erythroid differentiation. J Biochem 160:111-20 (2016).

2. Ni JZ et al. A transgenerational role of the germline nuclear RNAi pathway in repressing heat stress-induced transcriptional activation in C. elegans. Epigenetics Chromatin 9:3 (2016).

サブセルラー局在

Nucleus, Chromosome.

UNIPROT #

SwissProt: P68431 Human

SwissProt: P84243 Human

SwissProt: Q16695 Human

SwissProt: Q6NXT2 Human

SwissProt: Q71DI3 Human

SwissProt: P68433 Mouse

SwissProt: P84228 Mouse

SwissProt: Q6LED0 Rat

別名

H3 histone family, member A antibody

H3/A antibody

H31_HUMAN antibody

H3FA antibody

Hist1h3a antibody

HIST1H3B antibody

HIST1H3C antibody

HIST1H3D antibody

HIST1H3E antibody

HIST1H3F antibody

詳細を見る

H3 histone family, member A antibody

H3/A antibody

H31_HUMAN antibody

H3FA antibody

Hist1h3a antibody

HIST1H3B antibody

HIST1H3C antibody

HIST1H3D antibody

HIST1H3E antibody

HIST1H3F antibody

HIST1H3G antibody

HIST1H3H antibody

HIST1H3I antibody

HIST1H3J antibody

histone 1, H3a antibody

Histone cluster 1, H3a antibody

Histone H3.1 antibody

Histone H3/a antibody

Histone H3/b antibody

Histone H3/c antibody

Histone H3/d antibody

Histone H3/f antibody

Histone H3/h antibody

Histone H3/i antibody

Histone H3/j antibody

Histone H3/k antibody

Histone H3/l antibody

閉じる

画像

Western blot analysis of Histone H3 on different lysates with Mouse anti-Histone H3 antibody (HA724276H) at 1/10,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: RAW264.7 cell lysate
Lane 5: C6 cell lysate
Lane 6: PC-12 cell lysate
Lane 7: COS-1 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 15 kDa
Observed band size: 15 kDa

Exposure time: 10 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724276H, HRP) at 1/10,000 dilution was used in primary antibody dilution buffer (K1803) at room temperature for 1 hour.