Formyl-Histone H3 (K122) Recombinant Rabbit Monoclonal Antibody [PSH27-72]

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Catalog# HA724717

Formyl-Histone H3 (K122) Recombinant Rabbit Monoclonal Antibody [PSH27-72]

Application

WB
IHC-P

Reactivity

Human
Mouse
Rat

Conjugation

unconjugated

製品概要

製品名

Formyl-Histone H3 (K122) Recombinant Rabbit Monoclonal Antibody [PSH27-72]

抗体のタイプ

Recombinant Rabbit monoclonal Antibody

交差反応性（対応種属）

Human, Mouse, Rat

検証済みアプリケーション

WB, IHC-P

標的分子量

Predicted band size: 15 kDa

ポジティブコントロール

A549 (Human lung adenocarcinoma cell) cell lysate, HeLa (Human cervical adenocarcinoma cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate.

コンジュゲーション

unconjugated

クローン番号

PSH27-72

Reactivity Data

Tested

検証済（社内検証通過）

Published

文献報告済（社内未検証、文献サポートあり）

Predicted

反応性予測（高い相同性に基づく）

Not recommended

非推奨（社内検証未通過）

	WB	IHC-P
human
mouse
rat

製品の特徴

形態

Liquid

濃度

ロット濃度照会

保存方法

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

保存バッファー

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

アイソタイプ

IgG

精製方法

Protein A affinity purified.

推奨希釈倍率

WB
1:500

IHC-P
1:500

ターゲット

機能

Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fibers. Two molecules of each of the four core histones (H2A, H2B, H3 and H4) form the octamer, which is comprised of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Human and mouse Histone H4 are subject to methylation at Lys 20, a modification that may be necessary for select DNA transactions or chromatin state transitions.

背景・参考文献

1. Wilson J.P. et. al. Proteomic analysis of fatty-acylated proteins in mammalian cells with chemical reporters reveals S-acylation of histone H3 variants. Mol. Cell. Proteomics 10:M110.001198-M110.001198(2011).

サブセルラー局在

Chromosome, Nucleosome core, Nucleus.

UNIPROT #

SwissProt: P68431 Human

SwissProt: P84243 Human

SwissProt: Q16695 Human

SwissProt: Q71DI3 Human

SwissProt: Q6NXT2 Human

別名

H3 histone family member E pseudogene antibody

H3 histone family, member A antibody

H3/A antibody

H31_HUMAN antibody

H3F3 antibody

H3FA antibody

Hist1h3a antibody

HIST1H3B antibody

HIST1H3C antibody

HIST1H3D antibody

詳細を見る

画像

Western blot analysis of Formyl-Histone H3 (K122) on different lysates with Rabbit anti-Formyl-Histone H3 (K122) antibody (HA724717) at 1/500 dilution.

Lane 1: A549 (Human lung adenocarcinoma cell) cell lysate
Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 3: NIH/3T3 (Mouse fibroblast) cell lysate

Lysates/proteins at 20 µg/Lane.
Exposure time: 3 minutes ; ECL: K1802

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724717, 1/500 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 15 kDa
Observed band size: 15 kDa
Application: Immunohistochemistry (IHC-P)

Species: Human
Tissue: Colon
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724717, 1/500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
Application: Immunohistochemistry (IHC-P)

Species: Mouse
Tissue: Colon
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724717, 1/500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
Application: Immunohistochemistry (IHC-P)

Species: Rat
Tissue: Colon
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724717, 1/500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.

ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）

Formyl-Histone H3 (K122) Recombinant Rabbit Monoclonal Antibody [PSH27-72]

お問い合わせ

製品概要

製品名

抗体のタイプ

交差反応性（対応種属）

検証済みアプリケーション

標的分子量

ポジティブコントロール

コンジュゲーション

クローン番号

Reactivity Data

製品の特徴

形態

濃度

保存方法

保存バッファー

アイソタイプ

精製方法

推奨希釈倍率

ターゲット

機能

背景・参考文献

サブセルラー局在

UNIPROT #

別名

画像

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