Histone H3 Rabbit Polyclonal Antibody

製品仕様

50μl

100μl

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Catalog# HA500298

Histone H3 Rabbit Polyclonal Antibody

Application

Reactivity

Zebrafish
Human
Mouse

This product has been cited in peer reviewed publications, see list HERE

概要

製品名

Histone H3 Rabbit Polyclonal Antibody

抗体の種類

Rabbit Polyclonal Antibody

免疫原

Synthetic peptide within Zebrafish Histone H3 aa 1-50 / 136.

種属反応性

Zebrafish, Human, Mouse

検証された応用例

分子量

Predicted band size: 15 kDa

陽性対照

Zebrafish tissue lysates.

結合

unconjugated

RRID

AB_3071393

製品の特徴

形態

Liquid

保存方法

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

保存用バッファー

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

アイソタイプ

IgG

精製方法

Immunogen affinity purified.

応用希釈度

WB
1:500-1:5,000

論文における応用例

WB	確認する 1 以下の論文

論文における種属

Human

確認する 1 以下の論文

ターゲット

機能

Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

背景文献

1. Jacob Y.et.al.Selective methylation of histone H3 variant H3.1 regulates heterochromatin replication.Science 343:1249-1253(2014).

亜細胞局在

Nucleus,Chromosome.

UNIPROT #

SwissProt: Q6PI20 Zebrafish

SwissProt: Q4QRF4 Zebrafish

SwissProt: B2GSF5 Zebrafish

別名

H3 histone family member E pseudogene antibody

H3 histone family, member A antibody

H3/A antibody

H31_HUMAN antibody

H3F3 antibody

H3FA antibody

Hist1h3a antibody

HIST1H3B antibody

HIST1H3C antibody

HIST1H3D antibody

展開

画像

Western blot analysis of Histone H3 on zebrafish tissue lysates with Rabbit anti-Histone H3 antibody (HA500298) at 1/5,000 dilution.

Lysates/proteins at 10 µg/Lane.

Predicted band size: 15 kDa
Observed band size: 15 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500298) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

ご注意ください: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

論文での実績

Filter:

Linear ubiquitination regulates the KSHV replication and transcription activator protein to control infection

Author: Luan Yi,et al

PMID: 38951495
Journal: Nature Communications
- アプリケーション: WB
- 交差性: Human
- 掲載日: 2024 Jul
- Citation

Histone H3 Rabbit Polyclonal Antibody

製品仕様

お問い合わせ

概要

製品名

抗体の種類

免疫原

種属反応性

検証された応用例

分子量

陽性対照

結合

RRID

製品の特徴

形態

保存方法

保存用バッファー

アイソタイプ

精製方法

応用希釈度

論文における応用例

論文における種属

ターゲット

機能

背景文献

亜細胞局在

UNIPROT #

別名

画像

論文での実績

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