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HRP Conjugated Goat anti-Rabbit IgG polyclonal Antibody

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Catalog# HA1001

HRP Conjugated Goat anti-Rabbit IgG polyclonal Antibody

Application

  • WB

  • ELISA

  • IHC-P

Reactivity

  • Rabbit

Conjugation

  • HRP

This product has been cited in peer reviewed publications, see list HERE

製品概要

製品名

HRP Conjugated Goat anti-Rabbit IgG polyclonal Antibody

抗体のタイプ

Goat Polyclonal Antibody

免疫原

Rabbit IgG (H+L).

製品の特異性

To minimize cross-reactivity, the HRP conjugated goat anti-rabbit IgG (H+L) antibodies have been highly cross-adsorbed against human IgG and Mouse IgG.

交差反応性（対応種属）

Rabbit

検証済みアプリケーション

WB, ELISA, IHC-P

コンジュゲーション

HRP

製品の特徴

形態

Liquid

濃度

保存方法

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

保存バッファー

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

アイソタイプ

IgG

精製方法

Immunogen affinity purified.

推奨希釈倍率

  • WB

  • 1:50,000~1:100,000

  • ELISA

  • 1:5,000~1:10,000

  • IHC-P

  • 1:200-1:1,000

ターゲット

機能

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.

背景・参考文献

暂无

画像

  • Western blot analysis of rabbit anti-GAPDH antibody (<a href="/products/ET1601-4" style="font-weight: bold;text-decoration: underline;">ET1601-4</a>,1/2000) and rabbit anti-GST3 antibody (<a href="/products/ER1802-63" style="font-weight: bold;text-decoration: underline;">ER1802-63</a>, 1/1000) with K562 Cell and A549 Cell lysates (All lanes proteins at 10 µg per lane). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat anti-rabbit IgG -HRP) (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) was used for 1 hour at room temperature at 1:40,000 (Lane A), 1:80,000 (Lane B), 1:160,000 (Lane C) and 1:320,000 (Lane D).<br />Exposure time: 60 seconds

    Western blot analysis of rabbit anti-GAPDH antibody (ET1601-4,1/2000) and rabbit anti-GST3 antibody (ER1802-63, 1/1000) with K562 Cell and A549 Cell lysates (All lanes proteins at 10 µg per lane). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat anti-rabbit IgG -HRP) (HA1001) was used for 1 hour at room temperature at 1:40,000 (Lane A), 1:80,000 (Lane B), 1:160,000 (Lane C) and 1:320,000 (Lane D).
    Exposure time: 60 seconds

  • Direct ELISA analysis of Goat Anti-Rabbit IgG (H+L) was performed by coating wells of a 96-well plate with 50 µl per well of Rabbit IgG / Human IgG / Mouse IgG / Rat IgG / Guinea pig IgG diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1%BSA blocking buffer, and incubated with 100 µl per well of Goat Anti-Rabbit IgG (H+L) polyclonal antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) diluted at a concentration of 62.5 ng/mL for 1 hours at room temperature. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

    Direct ELISA analysis of Goat Anti-Rabbit IgG (H+L) was performed by coating wells of a 96-well plate with 50 µl per well of Rabbit IgG / Human IgG / Mouse IgG / Rat IgG / Guinea pig IgG diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1%BSA blocking buffer, and incubated with 100 µl per well of Goat Anti-Rabbit IgG (H+L) polyclonal antibody (HA1001) diluted at a concentration of 62.5 ng/mL for 1 hours at room temperature. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）

文献・引用論文

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