VGLUT2 Recombinant Rabbit Monoclonal Antibody [PSH10-54]
Catalog# HA723218
VGLUT2 Recombinant Rabbit Monoclonal Antibody [PSH10-54]
-
WB
-
IHC-Fr
-
IHC-P
-
IF-Tissue
-
Mouse
-
Rat
-
HA751357
不含抗保成分
-
Cynomolgus monkey
-
Pig
予測される交差反応性
-
unconjugated
製品概要
製品名
VGLUT2 Recombinant Rabbit Monoclonal Antibody [PSH10-54]
抗体のタイプ
Recombinant Rabbit monoclonal Antibody
交差反応性（対応種属）
Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
予測される交差反応性
検証済みアプリケーション
WB, IHC-Fr, IHC-P, IF-Tissue
標的分子量
Predicted band size: 65 kDa
ポジティブコントロール
Mouse brain tissue, mouse hippocampus tissue, mouse cerebellum tissue, rat brain tissue, rat hippocampus tissue, rat cerebellum tissue, Mouse brain tissue lysate, Rat brain tissue lysate.
コンジュゲーション
unconjugated
クローン番号
PSH10-54
Reactivity Data
| WB | IHC-Fr | IHC-P | IF-Tissue | |
|---|---|---|---|---|
| Mouse |
|
|
|
|
| Rat |
|
|
|
|
| Cynomolgus Monkey |
|
|
|
|
| Pig |
|
|
|
|
製品の特徴
形態
Liquid
濃度
保存方法
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
保存バッファー
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
アイソタイプ
IgG
精製方法
Protein A affinity purified.
推奨希釈倍率
-
WB
-
1:2,000
-
IHC-Fr
-
1:200-1:500
-
IHC-P
-
1:2,000
-
IF-Tissue
-
1:500
ターゲット
機能
Predicted to enable L-glutamate transmembrane transporter activity and neurotransmitter transmembrane transporter activity. Involved in neurotransmitter loading into synaptic vesicle. Predicted to be located in synaptic vesicle. Predicted to be active in excitatory synapse. Predicted to be integral component of synaptic vesicle membrane.
背景・参考文献
1. Buck SA et al. Roles of VGLUT2 and Dopamine/Glutamate Co-Transmission in Selective Vulnerability to Dopamine Neurodegeneration. ACS Chem Neurosci. 2022 Jan
2. Zhang Y et al. VGluT2 neuron subtypes in the paraventricular thalamic nucleus regulate depression in paraquat-induced Parkinson\'s disease. J Hazard Mater. 2024 Jul
サブセルラー局在
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Synapse, synaptosome, Cell membrane.
UNIPROT #
別名
Differentiation associated BNPI antibody
Differentiation associated Na dependent inorganic phosphate cotransporter antibody
Differentiation associated Na(+) dependent inorganic phosphate cotransporter antibody
Differentiation-associated BNPI antibody
Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter antibody
DNPI antibody
SLC17A6 antibody
Sodium dependent inorganic phosphate cotransporter antibody
Solute carrier family 17 (Sodium dependent inorganic phosphate cotransporter) member 6 antibody
Solute carrier family 17 member 6 antibody
詳細を見る画像
-
-
-
-
-
-
Application: IHC-Fr
Species: Mouse
Site: Cerebellum
Sample: Frozen section
Antibody concentration: 1: 500
Antigen retrieval: Not required -
Application: IHC-Fr
Species: Rat
Site: Cerebellum
Sample: Frozen section
Antibody concentration: 1: 500
Antigen retrieval: Not required -
Application: Immunofluorescence (IHC-Fr)
Species: Mouse
Tissue: Spinal cord
Sample: Frozen section
Antigen retrieval: Not required
Wash buffer: 1× PBS
Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.
Primary antibody: VGLUT2: 1/500 (HA723218, rabbit, Green), NeuN: 1/1,000 (HA601482, mouse, Red), overnight at 4℃.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), Goat anti-Mouse IgG (iFluor™ 594, HA1126), 1.5 hours at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-VGLUT2 antibody (HA723218) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723218) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-VGLUT2 antibody (HA723218) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723218) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-VGLUT2 antibody (HA723218) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723218) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-VGLUT2 antibody (HA723218) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723218) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-VGLUT2 antibody (HA723218) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723218) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-VGLUT2 antibody (HA723218) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723218) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Western blot analysis of VGLUT2 on different lysates with Rabbit anti-VGLUT2 antibody (HA723218) at 1/2,000 dilution.
Lane 1: Mouse brain tissue lysate (20 µg/Lane)
Lane 2: Mouse lung tissue lysate (negative) (20 µg/Lane)
Lane 3: Rat brain tissue lysate (20 µg/Lane)
Lane 4: Rat lung tissue lysate (negative) (20 µg/Lane)
Predicted band size: 65 kDa
Observed band size: 65 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723218) at 1/2,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）