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SLC32A1 / VGAT Recombinant Mouse Monoclonal Antibody [PSH10-47]

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Catalog# HA601384

SLC32A1 / VGAT Recombinant Mouse Monoclonal Antibody [PSH10-47]

Application

  • IHC-Fr

  • IHC-P

Reactivity

  • Mouse

  • Rat

BSA and Azide free
Predicted reactivity

  • Cynomolgus monkey

  • Pig

予測される種属に対するアフターサービスのサポート
Conjugation

  • unconjugated

概要

製品名

SLC32A1 / VGAT Recombinant Mouse Monoclonal Antibody [PSH10-47]

抗体の種類

Recombinant Mouse Monoclonal Antibody

種属反応性

Mouse, Rat (Predicted: Cynomolgus monkey, Pig)

予測される種属に対するアフターサービスのサポート

検証された応用例

IHC-Fr, IHC-P

分子量

Predicted band size: 57 kDa

陽性対照

Mouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue.

結合

unconjugated

クローン番号

PSH10-47

製品の特徴

形態

Liquid

保存方法

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

保存用バッファー

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

アイソタイプ

IgG1

精製方法

Protein A affinity purified.

応用希釈度

  • IHC-Fr

  • 1:500

  • IHC-P

  • 1:1,000

ターゲット

機能

Vesicular inhibitory amino acid transporter is a protein that in humans is encoded by the SLC32A1 gene. The protein encoded by this gene is an integral membrane protein involved in gamma-aminobutyric acid (GABA) and glycine uptake into synaptic vesicles. The encoded protein is a member of amino acid/polyamine transporter family II.

背景文献

1. Heron SE et al. Association of SLC32A1 Missense Variants With Genetic Epilepsy With Febrile Seizures Plus. Neurology. 2021 May

2. Guerrini R et al. SLC32A1: One More Gene Contributing to the Solution of the Genetic Generalized Epilepsies Mystery. Neurology. 2021 May

亜細胞局在

Cytoplasmic vesicle membrane, Presynapse.

UNIPROT #

SwissProt: O35633 Mouse

SwissProt: O35458 Rat

別名

bA122O1.1 antibody

GABA and glycine transporter antibody

hVIAAT antibody

SLC32A 1 antibody

Slc32a1 antibody

solute carrier family 32 (GABA vesicular transporter) member 1 antibody

Solute carrier family 32 member 1 antibody

Vesicular GABA Amino Acid Transporter antibody

Vesicular GABA transporter antibody

Vesicular inhibitory amino acid transporter antibody

展開

画像

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Hippocampus<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500 (SLC32A1 / VGAT, <a href="/products/HA601384" style="font-weight: bold;text-decoration: underline;">HA601384</a>, green); 1/500 (VGLUT1, <a href="/products/HA723217" style="font-weight: bold;text-decoration: underline;">HA723217</a>, red)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Hippocampus

    Sample: Frozen section

    Antibody concentration: 1/500 (SLC32A1 / VGAT, HA601384, green); 1/500 (VGLUT1, HA723217, red)

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Hippocampus<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500 (SLC32A1 / VGAT, <a href="/products/HA601384" style="font-weight: bold;text-decoration: underline;">HA601384</a>, green); 1/500 (VGLUT2, <a href="/products/HA723218" style="font-weight: bold;text-decoration: underline;">HA723218</a>, red)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Hippocampus

    Sample: Frozen section

    Antibody concentration: 1/500 (SLC32A1 / VGAT, HA601384, green); 1/500 (VGLUT2, HA723218, red)

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebellum <br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:500 (SLC32A1 / VGAT, <a href="/products/HA601384" style="font-weight: bold;text-decoration: underline;">HA601384</a>, green); 1:500 (Parvalbumin, <a href="/products/ET1703-15" style="font-weight: bold;text-decoration: underline;">ET1703-15</a>, red)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1:500 (SLC32A1 / VGAT, HA601384, green); 1:500 (Parvalbumin, ET1703-15, red)

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: Cerebellum <br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:500 (SLC32A1 / VGAT, <a href="/products/HA601384" style="font-weight: bold;text-decoration: underline;">HA601384</a>, green); 1:500 (Parvalbumin, <a href="/products/ET1703-15" style="font-weight: bold;text-decoration: underline;">ET1703-15</a>, red)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Rat

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1:500 (SLC32A1 / VGAT, HA601384, green); 1:500 (Parvalbumin, ET1703-15, red)

    Antigen retrieval: Not required

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-SLC32A1 / VGAT antibody (<a href="/products/HA601384" style="font-weight: bold;text-decoration: underline;">HA601384</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601384" style="font-weight: bold;text-decoration: underline;">HA601384</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-SLC32A1 / VGAT antibody (HA601384) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601384) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-SLC32A1 / VGAT antibody (<a href="/products/HA601384" style="font-weight: bold;text-decoration: underline;">HA601384</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601384" style="font-weight: bold;text-decoration: underline;">HA601384</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-SLC32A1 / VGAT antibody (HA601384) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601384) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-SLC32A1 / VGAT antibody (<a href="/products/HA601384" style="font-weight: bold;text-decoration: underline;">HA601384</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601384" style="font-weight: bold;text-decoration: underline;">HA601384</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-SLC32A1 / VGAT antibody (HA601384) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601384) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-SLC32A1 / VGAT antibody (<a href="/products/HA601384" style="font-weight: bold;text-decoration: underline;">HA601384</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601384" style="font-weight: bold;text-decoration: underline;">HA601384</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-SLC32A1 / VGAT antibody (HA601384) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601384) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ご注意ください: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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