NeuN Recombinant Antibody [SR45-07] - Mouse IgG1 (Chimeric)

Application: IHC-Fr

Species: Mouse

Site: brain

Sample: Frozen section

Antibody concentration: 1/1,000 (NeuN, HA601482, Mouse, green); 1/200 (QKI-6, HA601502, Guinea pig, red)

Antigen retrieval: Not required

Application: Immunofluorescence (IHC-Fr)

Species: Mouse
Tissue: Spinal cord
Sample: Frozen section

Antigen retrieval: Not required

Wash buffer: 1× PBS
Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.
Primary antibody: NeuN: 1/1,000 (HA601482, mouse, Red), VGLUT2: 1/500 (HA723218, rabbit, Green), overnight at 4℃.
Secondary antibody: Goat anti-Mouse IgG (iFluor™ 594, HA1126), Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature.

Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-NeuN antibody (HA601482) at 1/5,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA601482) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-NeuN antibody (HA601482) at 1/5,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA601482) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-NeuN antibody (HA601482) at 1/5,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA601482) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

☑ Relative expression (RE)

Western blot analysis of NeuN on different lysates with Mouse anti-NeuN antibody (HA601482) at 1/10,000 dilution.

Lane 1: Mouse brain tissue lysate
Lane 2: C2C12 cell lysate (negative)
Lane 3: Rat brain tissue lysate

Lysates/proteins at 15 µg/Lane.

Predicted band size: 34 kDa
Observed band size: 45/50 kDa

Exposure time: 20 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601482) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Application: Immunocytochemistry (IF-cell)

Species: Mouse
Sample: Mouse primary neuron cells

Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.

Primary antibody: HA601482, 1/100, overnight at 4℃.
Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 45 minutes at room temperature.
Counterstain: Beta tubulin (ET1602-4, Red), 1/500, overnight at 4℃. The nuclear counterstain was DAPI (Blue).