CD11b Recombinant Rabbit Monoclonal Antibody [JE01-17]
Catalog# HA722856
CD11b Recombinant Rabbit Monoclonal Antibody [JE01-17]
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WB
-
IHC-P
-
Human
-
Mouse
-
Rat
-
unconjugated
製品概要
製品名
CD11b Recombinant Rabbit Monoclonal Antibody [JE01-17]
抗体のタイプ
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within human CD11b aa 17-66 / 1,152.
交差反応性（対応種属）
Human, Mouse, Rat
検証済みアプリケーション
WB, IHC-P
分子量
Predicted band size: 127 kDa
ポジティブコントロール
RAW264.7 cell lysate, J774A.1 cell lysate, human cevical cancer tissue, human slpeen tissue, human tonsil tissue, mouse spleen tissue, rat spleen tissue.
コンジュゲーション
unconjugated
クローン番号
JE01-17
Reactivity Data
| WB | IHC-P | |
|---|---|---|
| Human |
|
|
| Mouse |
|
|
| Rat |
|
|
製品の特徴
形態
Liquid
濃度
保存方法
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
保存バッファー
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
アイソタイプ
IgG
精製方法
Protein A affinity purified.
推奨希釈倍率
-
WB
-
1:2,000
-
IHC-P
-
1:2,000-1:5,000
ターゲット
機能
Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens 1, 2. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration 3. In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils 4. May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity). Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development (By similarity).
背景・参考文献
1. Halfon M et al. ITGAM rs1143679 Variant in Systemic Lupus Erythematosus Is Associated with Increased Serum Calcification Propensity. Genes (Basel). 2023 May.
2. Sobieszek G et al. Polymorphism of the ITGAM gene (rs7193943) and bioelectric impedance analysis as potential predictors of cachexia in chronic heart failure. Sci Rep. 2021 Oct.
サブセルラー局在
Cell membrane, Membrane raft.
別名
antigen CD11b (p170)
Antigen CD11b p170
CD11 antigen like family member B
CD11 antigen-like family member B
CD11b
CD11b/CD18
CD49d
Cell surface glycoprotein MAC-1 subunit alpha
Complement component 3 receptor 3 subunit
Complement Component Receptor 3 Alpha
詳細を見る画像
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☑ Relative expression (RE)
Western blot analysis of CD11b on different lysates with Rabbit anti-CD11b antibody (HA722856) at 1/2,000 dilution.
Lane 1: RAW264.7 cell lysate (5 µg/Lane)
Lane 2: C2C12 cell lysate (negative) (10 µg/Lane)
Lane 3: J774A.1 cell lysate (5 µg/Lane)
Lane 4: Mouse spleen tissue lysate (20 µg/Lane)
Lane 5: Mouse heart tissue lysate (negative) (20 µg/Lane)
Lane 6: Rat spleen tissue lysate (20 µg/Lane)
Lane 7: Rat heart tissue lysate (negative) (20 µg/Lane)
Predicted band size: 127 kDa
Observed band size: 170 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722856) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human cevical cancer tissue with Rabbit anti-CD11b antibody (HA722856) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722856) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human slpeen tissue with Rabbit anti-CD11b antibody (HA722856) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722856) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD11b antibody (HA722856) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722856) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-CD11b antibody (HA722856) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722856) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-CD11b antibody (HA722856) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722856) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）
文献・引用論文
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Nerves Stimulates Crosstalk between Gastric Cancer and Group 3 Innate Lymphoid Cells to Enhance Immunosuppression
ジャーナル: Cancer Research
DOI: 10.1158/0008-5472.CAN-25-3092
IF: 16.6
アプリケーション: mIF
交差反応性: Human
掲載日: 2026 Jan
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