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CD11b Mouse Monoclonal Antibody [A2-9]

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Catalog# M1211-6

CD11b Mouse Monoclonal Antibody [A2-9]

Application

  • WB

  • IHC-P

  • FC

  • IF-Tissue

Reactivity

  • Human

Conjugation

  • unconjugated

製品概要

製品名

CD11b Mouse Monoclonal Antibody [A2-9]

抗体のタイプ

Mouse Monoclonal Antibody

免疫原

Recombinant protein within human CD11b 500-750 aa.

交差反応性（対応種属）

Human

検証済みアプリケーション

WB, IHC-P, FC, IF-Tissue

分子量

Predicted band size: 127 kDa

ポジティブコントロール

TF-1 cell lysate, human tonsil tissue, human spleen tissue, Raji.

コンジュゲーション

unconjugated

クローン番号

A2-9

RRID

AB_3073051

製品の特徴

形態

Liquid

濃度

保存方法

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

保存バッファー

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

アイソタイプ

IgG2b

精製方法

Protein A affinity purified.

推奨希釈倍率

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

  • FC

  • 1:200

  • IF-Tissue

  • 1:200

ターゲット

機能

Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.

背景・参考文献

1. "JAM-C is a component of desmosomes and a ligand for CD11b/CD18-mediated neutrophil transepithelial migration." Zen K., Babbin B.A., Liu Y., Whelan J.B., Nusrat A., Parkos C.A. Mol. Biol. Cell 15:3926-3937(2004)

2. Nath S.K., Han S "A nonsynonymous functional variant in integrin-alpha(M) (encoded by ITGAM) is associated with systemic lupus erythematosus."., Kim-Howard X., Kelly J.A., Viswanathan P., Gilkeson G.S., Chen W., Zhu C., McEver R.P., Kimberly R.P., Alarcon-Riquelme M.E., Vyse T.J., Li Q.-Z., Wakeland E.K., Merrill J.T., James J.A., Kaufman K.M., Guthridge J.M., Harley J.B. Nat. Genet. 40:152-154(2008)

配列相同性

Belongs to the integrin alpha chain family.

組織特異性

Predominantly expressed in monocytes and granulocytes. Expressed in neutrophils (at protein level).

サブセルラー局在

Membrane.

UNIPROT #

SwissProt: P11215 Human

別名

antigen CD11b (p170) antibody

Antigen CD11b p170 antibody

CD11 antigen like family member B antibody

CD11 antigen-like family member B antibody

CD11b antibody

CD11b/CD18 antibody

CD49d antibody

Cell surface glycoprotein MAC-1 subunit alpha antibody

Complement component 3 receptor 3 subunit antibody

Complement Component Receptor 3 Alpha antibody

詳細を見る

画像

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of CD11b on different lysates with Mouse anti-CD11b antibody (<a href="/products/M1211-6" style="font-weight: bold;text-decoration: underline;">M1211-6</a>) at 1/1,000 dilution.<br /><br />Lane 1: TF-1 cell lysate<br />Lane 2: Jurkat cell lysate (negative)<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 127 kDa<br />Observed band size: 170 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/M1211-6" style="font-weight: bold;text-decoration: underline;">M1211-6</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of CD11b on different lysates with Mouse anti-CD11b antibody (M1211-6) at 1/1,000 dilution.

    Lane 1: TF-1 cell lysate
    Lane 2: Jurkat cell lysate (negative)

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 127 kDa
    Observed band size: 170 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1211-6) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD11b antibody (<a href="/products/M1211-6" style="font-weight: bold;text-decoration: underline;">M1211-6</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M1211-6" style="font-weight: bold;text-decoration: underline;">M1211-6</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD11b antibody (M1211-6) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1211-6) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-CD11b antibody (<a href="/products/M1211-6" style="font-weight: bold;text-decoration: underline;">M1211-6</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M1211-6" style="font-weight: bold;text-decoration: underline;">M1211-6</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-CD11b antibody (M1211-6) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1211-6) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: IF-Tissue<br /><br />Species: Human<br /><br />Site: Spleen<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/200

    Application: IF-Tissue

    Species: Human

    Site: Spleen

    Sample: Paraffin-embedded section

    Antibody concentration: 1/200

ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）

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