CD11b Mouse Monoclonal Antibody [4-6]
Catalog# EM1701-42
CD11b Mouse Monoclonal Antibody [4-6]
-
IHC-P
-
IF-Cell
-
FC
-
WB
-
IF-Tissue
-
Human
-
unconjugated
製品概要
製品名
CD11b Mouse Monoclonal Antibody [4-6]
抗体のタイプ
Mouse Monoclonal Antibody
免疫原
Recombinant protein within Human CD11b aa 544-743 / 1,152.
交差反応性（対応種属）
Human
検証済みアプリケーション
IHC-P, IF-Cell, FC, WB, IF-Tissue
標的分子量
Predicted band size: 127 kDa
ポジティブコントロール
TF-1 cell lysate, THP-1 cell lysate, U-937 cell lysate, A431, LOVO, human tonsil tissue, human spleen tissue.
コンジュゲーション
unconjugated
クローン番号
4-6
RRID
製品の特徴
形態
Liquid
濃度
保存方法
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
保存バッファー
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
アイソタイプ
IgG2b
精製方法
Protein G affinity purified.
推奨希釈倍率
-
IF-Cell
-
1:50-1:200
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
-
WB
-
1:1,000
-
IF-Tissue
-
1:50
ターゲット
機能
Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration. In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils. May regulate phagocytosis-induced apoptosis in extravasated neutrophils. May play a role in mast cell development.
背景・参考文献
1. Jerke U et al.Complement receptor Mac-1 is an adaptor for NB1 (CD177)-mediated PR3-ANCA neutrophil activation. J. Biol. Chem. 286:7070-7081 (2011).
2. Bai M. et al. CD177 modulates human neutrophil migration through activation-mediated integrin and chemoreceptor regulation. Blood 0:0-0 (2017).
配列相同性
Belongs to the integrin alpha chain family.
組織特異性
Predominantly expressed in monocytes and granulocytes. Expressed in neutrophils (at protein level).
サブセルラー局在
Cell membrane.
UNIPROT #
別名
antigen CD11b (p170) antibody
Antigen CD11b p170 antibody
CD11 antigen like family member B antibody
CD11 antigen-like family member B antibody
CD11b antibody
CD11b/CD18 antibody
CD49d antibody
Cell surface glycoprotein MAC-1 subunit alpha antibody
Complement component 3 receptor 3 subunit antibody
Complement Component Receptor 3 Alpha antibody
詳細を見る画像
-
Western blot analysis of CD11b on different lysates with Mouse anti-CD11b antibody (EM1701-42) at 1/1,000 dilution.
Lane 1: TF-1 cell lysate
Lane 2: THP-1 cell lysate
Lane 3: U-937 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 127 kDa
Observed band size: 170 kDa
Exposure time: 30 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-42) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
ICC staining CD11b (green) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
ICC staining CD11b (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD11b antibody. Counter stained with hematoxylin.
-
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD11b antibody. Counter stained with hematoxylin.
-
Application: IF-Tissue
Species: Human
Site: Spleen
Sample: Paraffin-embedded section
Antibody concentration: 1/50
ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）
文献・引用論文
-
Optimized CAR-T therapy based on spatiotemporal changes and chemotactic mechanisms of MDSCs induced by hypofractionated radiotherapy
ジャーナル: Molecular Therapy
DOI:
IF: 12.4
アプリケーション: FC
交差反応性: Mouse
掲載日: 2023 Jul
Alternative Products
CD11b Recombinant Rabbit Monoclonal Antibody [JU93-81]
Application: WB,IP,IHC-P,IF-Cell,IF-Tissue,mIHC
Reactivity: Human
Conjugate: unconjugated
CD11b Recombinant Rabbit Monoclonal Antibody [PSH03-96]
Application: WB,IHC-P,IF-Tissue
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
CD11b Recombinant Rabbit Monoclonal Antibody [JE01-17]
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
同一ターゲット＆同一シグナル伝達経路の関連製品
CD11b Recombinant Rabbit Monoclonal Antibody [JU93-81] - BSA and Azide free
Application: WB,IP,IHC-P,IF-Cell,IF-Tissue
Reactivity: Human
Conjugate: unconjugated
CD11b Recombinant Rabbit Monoclonal Antibody
Application: mIHC
Reactivity: Human
Conjugate: unconjugated
CD11b Mouse Monoclonal Antibody [A2-9]
Application: WB,IHC-P,FC,IF-Tissue
Reactivity: Human
Conjugate: unconjugated
CD11b Recombinant Rabbit Monoclonal Antibody [PSH03-96] - BSA and Azide free
Application: WB,IHC-P,IF-Tissue
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
CD11b Mouse Monoclonal Antibody [A2-B5-D2]
Application: WB
Reactivity: Human,Mouse
Conjugate: unconjugated
CD11b Recombinant Rabbit Monoclonal Antibody [JU93-81]
Application: WB,IP,IHC-P,IF-Cell,IF-Tissue,mIHC
Reactivity: Human
Conjugate: unconjugated
CD11b Recombinant Rabbit Monoclonal Antibody [PSH03-96]
Application: WB,IHC-P,IF-Tissue
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated