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NeuN Recombinant Mouse Monoclonal Antibody [PD01-45]

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Catalog# HA601111

NeuN Recombinant Mouse Monoclonal Antibody [PD01-45]

Application

  • WB

  • IF-Tissue

  • IHC-P

  • IHC-Fr

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

製品概要

製品名

NeuN Recombinant Mouse Monoclonal Antibody [PD01-45]

抗体のタイプ

Recombinant Mouse Monoclonal Antibody

免疫原

Synthetic peptide within human NeuN aa 20-60.

交差反応性（対応種属）

Human, Mouse, Rat

検証済みアプリケーション

WB, IF-Tissue, IHC-P, IHC-Fr

分子量

Predicted band size: 34 kDa

ポジティブコントロール

SH-SY5Y cell lysate, SHG44 cell lysate, Human brain tissue, mouse hippocampus tissue, mouse brain tissue, mouse cerebellum tissue, rat hippocampus tissue, rat brain tissue, rat cerebellum tissue, mouse cerebellum tissue lysates, mouse cerebral cortex tissue.

コンジュゲーション

unconjugated

クローン番号

PD01-45

RRID

AB_3071833

製品の特徴

形態

Liquid

濃度

保存方法

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

保存バッファー

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

アイソタイプ

IgG1

精製方法

Protein A affinity purified.

推奨希釈倍率

  • WB

  • 1:1,000

  • IF-Tissue

  • 1:500

  • IHC-P

  • 1:1,000

  • IHC-Fr

  • 1:500-1:1,000

ターゲット

機能

Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, and dentate nucleus neurons. This neuronal protein was originally identified by immunoreactivity with a monoclonal antibody also called NeuN. Using MS-analysis, NeuN was later identified as the Fox-3 gene product. Fox-3 contains an RNA recognition motif and functions as a splicing regulator. Fox-3 regulates alternative splicing of NumB, promoting neuronal differentiation during development.

背景・参考文献

1. Patel TP et al. Single-neuron NMDA receptor phenotype influences neuronal rewiring and reintegration following traumatic injury. J Neurosci 34:4200-13 (2014).

2. Kaur P et al. Expression profiling of RNA transcripts during neuronal maturation and ischemic injury. PLoS One 9:e103525 (2014).

サブセルラー局在

Cytoplasm, Nucleus

UNIPROT #

SwissProt: A6NFN3 Human

SwissProt: Q8BIF2 Mouse

Unigene:143966 Rat

別名

FLJ56884 antibody

FLJ58356 antibody

Fox-1 homolog C antibody

fox1 homolog C antibody

Fox3 antibody

FOX3NeuN antibody

hexaribonucleotide binding protein 3 antibody

HRNBP3 antibody

NEUN antibody

neuronal nuclei antibody

詳細を見る

画像

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebellum<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500 (NeuN, <a href="/products/HA601111" style="font-weight: bold;text-decoration: underline;">HA601111</a>, Mouse, green); 1/500 (Iba1, <a href="/products/HA601368" style="font-weight: bold;text-decoration: underline;">HA601368</a>, Guinea pig, red)<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1/500 (NeuN, HA601111, Mouse, green); 1/500 (Iba1, HA601368, Guinea pig, red)

    Antigen retrieval: Not required

  • Western blot analysis of NeuN on mouse cerebellum tissue lysate with Mouse anti-NeuN antibody (<a href="/products/HA601111" style="font-weight: bold;text-decoration: underline;">HA601111</a>) at 1/1,000 dilution.<br /><br /><br />Lysates/proteins at 10 µg/Lane.<br />Exposure time: 20 seconds; ECL: K1801<br /><br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA601111" style="font-weight: bold;text-decoration: underline;">HA601111</a>, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃<br />Secondary antibody: Anti-mouse IgG for IP Nano-secondary antibody (<a href="/products/NBI02H" style="font-weight: bold;text-decoration: underline;">NBI02H</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 34 kDa<br />Observed band size: 45/50 kDa

    Western blot analysis of NeuN on mouse cerebellum tissue lysate with Mouse anti-NeuN antibody (HA601111) at 1/1,000 dilution.


    Lysates/proteins at 10 µg/Lane.
    Exposure time: 20 seconds; ECL: K1801


    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA601111, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
    Secondary antibody: Anti-mouse IgG for IP Nano-secondary antibody (NBI02H), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 34 kDa
    Observed band size: 45/50 kDa

  • Western blot analysis of NeuN on different lysates with Mouse anti-NeuN antibody (<a href="/products/HA601111" style="font-weight: bold;text-decoration: underline;">HA601111</a>) at 1/500 dilution.<br /><br />Lane 1: SH-SY5Y cell lysate<br />Lane 2: SHG44 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 34 kDa<br />Observed band size: 50 kDa<br /><br />Exposure time: 2 minutes;<br /><br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601111" style="font-weight: bold;text-decoration: underline;">HA601111</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:150,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of NeuN on different lysates with Mouse anti-NeuN antibody (HA601111) at 1/500 dilution.

    Lane 1: SH-SY5Y cell lysate
    Lane 2: SHG44 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 34 kDa
    Observed band size: 50 kDa

    Exposure time: 2 minutes;

    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601111) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-NeuN antibody (<a href="/products/HA601111" style="font-weight: bold;text-decoration: underline;">HA601111</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601111" style="font-weight: bold;text-decoration: underline;">HA601111</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-NeuN antibody (HA601111) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601111) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Mouse anti-NeuN antibody (<a href="/products/HA601111" style="font-weight: bold;text-decoration: underline;">HA601111</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601111" style="font-weight: bold;text-decoration: underline;">HA601111</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Mouse anti-NeuN antibody (HA601111) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601111) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-NeuN antibody (<a href="/products/HA601111" style="font-weight: bold;text-decoration: underline;">HA601111</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601111" style="font-weight: bold;text-decoration: underline;">HA601111</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-NeuN antibody (HA601111) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601111) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-NeuN antibody (<a href="/products/HA601111" style="font-weight: bold;text-decoration: underline;">HA601111</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601111" style="font-weight: bold;text-decoration: underline;">HA601111</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-NeuN antibody (HA601111) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601111) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Mouse anti-NeuN antibody (<a href="/products/HA601111" style="font-weight: bold;text-decoration: underline;">HA601111</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601111" style="font-weight: bold;text-decoration: underline;">HA601111</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Mouse anti-NeuN antibody (HA601111) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601111) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-NeuN antibody (<a href="/products/HA601111" style="font-weight: bold;text-decoration: underline;">HA601111</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601111" style="font-weight: bold;text-decoration: underline;">HA601111</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-NeuN antibody (HA601111) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601111) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-NeuN antibody (<a href="/products/HA601111" style="font-weight: bold;text-decoration: underline;">HA601111</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601111" style="font-weight: bold;text-decoration: underline;">HA601111</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-NeuN antibody (HA601111) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601111) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: IF-tissue<br /><br />Species: Mouse<br /><br />Site: Cerebral cortex<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/500

    Application: IF-tissue

    Species: Mouse

    Site: Cerebral cortex

    Sample: Paraffin-embedded section

    Antibody concentration: 1/500

  • Application: IF-tissue<br /><br />Species: Rat<br /><br />Site: Cerebral cortex<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/500

    Application: IF-tissue

    Species: Rat

    Site: Cerebral cortex

    Sample: Paraffin-embedded section

    Antibody concentration: 1/500

ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）

文献・引用論文

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