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Iba1 Recombinant Antibody [JM36-62] - Rat IgG1 (Chimeric)

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Catalog# HA601367

Iba1 Recombinant Antibody [JM36-62] - Rat IgG1 (Chimeric)

Application

  • IHC-Fr

  • IHC-P

  • mIHC

  • IF-Cell

Reactivity

  • Mouse

  • Rat

BSA and Azide free
Predicted reactivity

  • Cynomolgus monkey

  • Pig

予測される交差反応性
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

製品概要

製品名

Iba1 Recombinant Antibody [JM36-62] - Rat IgG1 (Chimeric)

抗体のタイプ

Recombinant Chimeric Antibody

免疫原

Synthetic peptide within N-terminal human Iba1.

製品の特異性

This Rat Recombinant monoclonal chimeric antibody has been engineered from a HiMab parent antibody (ET1705-78). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed FC-reactive secondary antibodies are recommended.

交差反応性（対応種属）

Mouse, Rat (Predicted: Cynomolgus monkey, Pig)

予測される交差反応性

検証済みアプリケーション

IHC-Fr, IHC-P, mIHC, IF-Cell

分子量

Predicted band size: 17 kDa

ポジティブコントロール

Mouse brain tissue, mouse striatum tissue, rat striatum tissue.

コンジュゲーション

unconjugated

クローン番号

JM36-62

Reactivity Data

IHC-Fr IHC-P mIHC IF-Cell
Mouse
Tested Tested
Tested Tested
Rat
Tested Tested
Tested Tested
Cynomolgus Monkey
Published
Published
Published
Published
Pig
Published
Published
Published
Published

製品の特徴

形態

Liquid

濃度

保存方法

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

保存バッファー

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

精製方法

Protein A affinity purified.

推奨希釈倍率

  • IHC-Fr

  • 1:500-1:1,000

  • IHC-P

  • 1:100-1:500

  • mIHC

  • 1:500

  • IF-Cell

  • 1:100

ターゲット

機能

Ionized calcium-binding adapter molecule 1 (Iba1), also known as allograft inflammatory factor-1 (AIF-1), is a 147 amino acid cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. Iba1, containing two EF domains, is induced by cytokines and interferons. In an unstimulated state, Iba1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding Iba1 maps to chromosome 6p21.33 and resides in the tumor necrosis factor (TNF) cluster of genes located in the region represented by the human major histocompatibility complex (MHC).

背景・参考文献

1. Hennessy E et al. Systemic TNF-a produces acute cognitive dysfunction and exaggerated sickness behavior when superimposed upon progressive neurodegeneration. Brain Behav Immun 59:233-244 (2017).

2. Arentsen T et al. The bacterial peptidoglycan-sensing molecule Pglyrp2 modulates brain development and behavior. Mol Psychiatry 22:257-266 (2017).

サブセルラー局在

Cytoplasm, cytoskeleton, Cell projection, ruffle membrane, Cell projection, phagocytic cup.

UNIPROT #

SwissProt: P55008 Human

SwissProt: O70200 Mouse

SwissProt: P55009 Rat

別名

AIF 1 antibody

AIF-1 antibody

Aif1 antibody

AIF1 protein antibody

AIF1_HUMAN antibody

Allograft inflammatory factor 1 antibody

Allograft inflammatory factor 1 splice variant G antibody

allograft inflammatory factor-1 splice variant Hara-1 antibody

balloon angioplasty responsive transcription antibody

BART 1 antibody

詳細を見る

画像

  • Application: Immunofluorescence (IHC-Fr)<br /><br />Species: Mouse<br />Tissue: Cerebral cortex<br />Sample: Frozen section<br /><br />Antigen retrieval: Not required<br /><br />Wash buffer: 1× PBST<br />Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.<br />Primary antibody: <a href="/products/HA601367" style="font-weight: bold;text-decoration: underline;">HA601367</a>, 1/500, overnight at 4℃.<br />Secondary antibody: Goat Anti-Rat IgG (iFluor&trade; 488, <a href="/products/HA1133" style="font-weight: bold;text-decoration: underline;">HA1133</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IHC-Fr)

    Species: Mouse
    Tissue: Cerebral cortex
    Sample: Frozen section

    Antigen retrieval: Not required

    Wash buffer: 1× PBST
    Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.
    Primary antibody: HA601367, 1/500, overnight at 4℃.
    Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 1.5 hours at room temperature.

  • Application: Immunofluorescence (IHC-Fr)<br /><br />Species: Mouse<br />Tissue: Cerebellum<br />Sample: Frozen section<br /><br />Antigen retrieval: Not required<br /><br />Wash buffer: 1× PBST<br />Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.<br />Primary antibody: Iba1, 1/1,000 (<a href="/products/HA601367" style="font-weight: bold;text-decoration: underline;">HA601367</a>, rat, Green), NeuN, 1:1,000 (<a href="/products/ET1602-12" style="font-weight: bold;text-decoration: underline;">ET1602-12</a>, rabbit, Red), overnight at 4℃.<br />Secondary antibody: Goat Anti-Rat IgG (iFluor&trade; 488, <a href="/products/HA1133" style="font-weight: bold;text-decoration: underline;">HA1133</a>), Goat anti-Rabbit IgG (iFluor&trade; 594, <a href="/products/HA1122" style="font-weight: bold;text-decoration: underline;">HA1122</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IHC-Fr)

    Species: Mouse
    Tissue: Cerebellum
    Sample: Frozen section

    Antigen retrieval: Not required

    Wash buffer: 1× PBST
    Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.
    Primary antibody: Iba1, 1/1,000 (HA601367, rat, Green), NeuN, 1:1,000 (ET1602-12, rabbit, Red), overnight at 4℃.
    Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), Goat anti-Rabbit IgG (iFluor™ 594, HA1122), 1.5 hours at room temperature.

  • Application: Immunofluorescence (IHC-Fr)<br /><br />Species: Mouse<br />Tissue: Cerebellum<br />Sample: Frozen section<br /><br />Antigen retrieval: Not required<br /><br />Wash buffer: 1× PBST<br />Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.<br />Primary antibody: Iba1, 1/500 (<a href="/products/HA601367" style="font-weight: bold;text-decoration: underline;">HA601367</a>, rat, Green), GLAST /EAAT1, 1:1,000 (<a href="/products/ET1704-54" style="font-weight: bold;text-decoration: underline;">ET1704-54</a>, rabbit, Red), overnight at 4℃.<br />Secondary antibody: Goat Anti-Rat IgG (iFluor&trade; 488, <a href="/products/HA1133" style="font-weight: bold;text-decoration: underline;">HA1133</a>), Goat anti-Rabbit IgG (iFluor&trade; 594, <a href="/products/HA1122" style="font-weight: bold;text-decoration: underline;">HA1122</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IHC-Fr)

    Species: Mouse
    Tissue: Cerebellum
    Sample: Frozen section

    Antigen retrieval: Not required

    Wash buffer: 1× PBST
    Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.
    Primary antibody: Iba1, 1/500 (HA601367, rat, Green), GLAST /EAAT1, 1:1,000 (ET1704-54, rabbit, Red), overnight at 4℃.
    Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), Goat anti-Rabbit IgG (iFluor™ 594, HA1122), 1.5 hours at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /><br />Species: Mouse <br />Tissue: Cerebral cortex<br />Sample: Paraffin-embedded section <br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. <br /><br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA601367" style="font-weight: bold;text-decoration: underline;">HA601367</a>, 1/500, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1023" style="font-weight: bold;text-decoration: underline;">HA1023</a>, 30 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: Cerebral cortex
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601367, 1/500, 1 hour at room temperature.
    Secondary antibody: HA1023, 30 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /><br />Species: Mouse <br />Tissue: Striatum<br />Sample: Paraffin-embedded section <br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. <br /><br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA601367" style="font-weight: bold;text-decoration: underline;">HA601367</a>, 1/100, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1023" style="font-weight: bold;text-decoration: underline;">HA1023</a>, 30 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: Striatum
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601367, 1/100, 1 hour at room temperature.
    Secondary antibody: HA1023, 30 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /><br />Species: Rat<br />Tissue: Striatum<br />Sample: Paraffin-embedded section <br /><br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. <br /><br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA601367" style="font-weight: bold;text-decoration: underline;">HA601367</a>, 1/200, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1023" style="font-weight: bold;text-decoration: underline;">HA1023</a>, 30 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Rat
    Tissue: Striatum
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601367, 1/200, 1 hour at room temperature.
    Secondary antibody: HA1023, 30 minutes at room temperature.

  • Application: Immunocytochemistry (IF-cell)<br /><br />Species: Mouse<br />Sample: BV2 (Mouse microglial cells)<br /><br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.<br />Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.<br /><br />Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.<br />Antibody dilution buffer: 1% BSA in PBST.<br />Primary antibody: <a href="/products/HA601367" style="font-weight: bold;text-decoration: underline;">HA601367</a>, 1/100, overnight at 4℃.<br />Secondary antibody: Goat Anti-Rat IgG (iFluor&trade; 488, <a href="/products/HA1133" style="font-weight: bold;text-decoration: underline;">HA1133</a>), 45 minutes at room temperature.<br /><br />Counterstain: Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue).

    Application: Immunocytochemistry (IF-cell)

    Species: Mouse
    Sample: BV2 (Mouse microglial cells)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.

    Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
    Antibody dilution buffer: 1% BSA in PBST.
    Primary antibody: HA601367, 1/100, overnight at 4℃.
    Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 45 minutes at room temperature.

    Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue).

  • Application: Immunocytochemistry (IF-cell)<br /><br />Species: Mouse<br />Sample: RAW264.7 (Mouse monocytic macrophage leukemia cells)<br /><br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.<br />Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.<br /><br />Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.<br />Antibody dilution buffer: 1% BSA in PBST.<br />Primary antibody: <a href="/products/HA601367" style="font-weight: bold;text-decoration: underline;">HA601367</a>, 1/100, overnight at 4℃.<br />Secondary antibody: Goat Anti-Rat IgG (iFluor&trade; 488, <a href="/products/HA1133" style="font-weight: bold;text-decoration: underline;">HA1133</a>), 45 minutes at room temperature.<br /><br />Counterstain: Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue).

    Application: Immunocytochemistry (IF-cell)

    Species: Mouse
    Sample: RAW264.7 (Mouse monocytic macrophage leukemia cells)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.

    Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
    Antibody dilution buffer: 1% BSA in PBST.
    Primary antibody: HA601367, 1/100, overnight at 4℃.
    Secondary antibody: Goat Anti-Rat IgG (iFluor™ 488, HA1133), 45 minutes at room temperature.

    Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue).

ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）

文献・引用論文

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