Iba1 Recombinant Antibody [JM36-62] - Guinea pig IgG2 (Chimeric)
概要
製品名
Iba1 Recombinant Antibody [JM36-62] - Guinea pig IgG2 (Chimeric)
抗体の種類
Recombinant Chimeric Antibody
免疫原
Synthetic peptide within N-terminal human Iba1.
製品の特異性
This Guinea pig Recombinant monoclonal chimeric antibody has been engineered from a HiMab parent antibody (ET1705-78). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed FC-reactive secondary antibodies are recommended.
種属反応性
Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
予測される種属に対するアフターサービスのサポート
検証された応用例
IHC-Fr, WB
分子量
Predicted band size: 17 kDa
陽性対照
Mouse brain tissue, mouse hippocampus tissue, rat brain tissue, THP-1 cell lysates.
結合
unconjugated
クローン番号
JM36-62
製品の特徴
形態
Liquid
保存方法
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
保存用バッファー
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
精製方法
Protein A affinity purified.
応用希釈度
-
IHC-Fr
-
1:200-1:500
-
WB
-
1:1,000
ターゲット
機能
Ionized calcium-binding adapter molecule 1 (Iba1), also known as allograft inflammatory factor-1 (AIF-1), is a 147 amino acid cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. Iba1, containing two EF domains, is induced by cytokines and interferons. In an unstimulated state, Iba1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding Iba1 maps to chromosome 6p21.33 and resides in the tumor necrosis factor (TNF) cluster of genes located in the region represented by the human major histocompatibility complex (MHC).
背景文献
1. Hennessy E et al. Systemic TNF-a produces acute cognitive dysfunction and exaggerated sickness behavior when superimposed upon progressive neurodegeneration. Brain Behav Immun 59:233-244 (2017).
2. Arentsen T et al. The bacterial peptidoglycan-sensing molecule Pglyrp2 modulates brain development and behavior. Mol Psychiatry 22:257-266 (2017).
亜細胞局在
Cytoplasm, cytoskeleton, Cell projection, ruffle membrane, Cell projection, phagocytic cup.
別名
AIF 1 antibody
AIF-1 antibody
Aif1 antibody
AIF1 protein antibody
AIF1_HUMAN antibody
Allograft inflammatory factor 1 antibody
Allograft inflammatory factor 1 splice variant G antibody
allograft inflammatory factor-1 splice variant Hara-1 antibody
balloon angioplasty responsive transcription antibody
BART 1 antibody
展開画像
-
Application: IHC-Fr
Species: Mouse
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
-
Application: IHC-Fr
Species: Mouse
Site: Hippocampus
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
Application: IHC-Fr
Species: Rat
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
Western blot analysis of Iba1 on THP-1 cell lysates with Guinea pig anti-Iba1 antibody (HA601368) at 1/1,000 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 17 kDa
Observed band size: 12 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601368) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Rabbit anti-Guinea pig IgG - HRP Secondary Antibody (HA1021) at 1/5,000 dilution was used for 1 hour at room temperature.
ご注意ください: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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