Skip to content

beta Tubulin Recombinant Rabbit Monoclonal Antibody [SR25-04]

RMB: 599 特別割引 1500

製品仕様

今すぐ注文，【现货】

問い合わせをクリック

お問い合わせ

メール: [email protected]

電話: 0571-88062880

代理店を調べる

Catalog# ET1602-4

beta Tubulin Recombinant Rabbit Monoclonal Antibody [SR25-04]

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

This product has been cited in peer reviewed publications, see list HERE

概要

製品名

beta Tubulin Recombinant Rabbit Monoclonal Antibody [SR25-04]

抗体の種類

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within Human beta Tubulin aa 308-357 / 444.

種属反応性

Human, Mouse, Rat

検証された応用例

WB, IF-Cell, IHC-P, FC

分子量

Predicted band size: 50 kDa

陽性対照

HeLa cell lysate, 293T cell lysate, MCF7 cell lysate, SH-SY5Y cell lysate, U-2 OS cell lysate, Jurkat cell lysate, Neuro-2a cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, SH-SY5Y, Hela, NIH/3T3, CRC, N2A, PC-12, human fallopian tube tissue, human colon carcinoma tissue, rat kidney tissue, mouse large intestine tissue.

結合

unconjugated

クローン番号

SR25-04

RRID

AB_2938592

製品の特徴

形態

Liquid

濃度

1ug/ul

保存方法

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

保存用バッファー

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

アイソタイプ

IgG

精製方法

Protein A affinity purified.

応用希釈度

  • WB

  • 1:20,000-1:100,000

  • IF-Cell

  • 1:200-1:500

  • IHC-P

  • 1:400-1:1,000

  • FC

  • 1:500-1:1,000

ターゲット

機能

Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin. The beta-tubulin (relative molecular weight about 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain. Beta-Tubulin may have bound GTP or GDP. Under certain conditions β-tubulin can hydrolyze its bound GTP to GDP plus Pi, release the Pi, and exchange the GDP for GTP.

背景文献

1. "Tumoral and tissue-specific expression of the major human beta-tubulin isotypes."Leandro-Garcia L.J., Leskela S., Landa I., Montero-Conde C., Lopez-Jimenez E., Leton R., .Cytoskeleton 67:214-223(2010).

2. "Five mouse tubulin isotypes and their regulated expression during development."Lewis S.A., Lee M.G.-S., Cowan N.J.J. Cell Biol. 101:852-861(1985).

配列相同性

Belongs to the tubulin family.

組織特異性

Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

翻訳後修飾

Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold.; Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).; Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.

亜細胞局在

Cytoplasm

UNIPROT #

SwissProt: P07437 Human

SwissProt: P99024 Mouse

SwissProt: P69897 Rat

別名

Beta 4 tubulin antibody

Beta 5 tubulin antibody

BetaTubulin antibody

TBB5_HUMAN antibody

TUBB antibody

TUBB2 antibody

TUBB2A antibody

TUBB5 antibody

tubulin beta 2A antibody

Tubulin beta chain antibody

展開

画像

  • Western blot analysis of beta Tubulin on different lysates with Rabbit anti-beta Tubulin antibody (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>) at 1/20,000 dilution.<br /><br />Lane 1: HeLa cell lysate (15 µg/Lane)<br />Lane 2: 293T cell lysate (15 µg/Lane)<br />Lane 3: MCF7 cell lysate (15 µg/Lane)<br />Lane 4: SH-SY5Y cell lysate (15 µg/Lane)<br />Lane 5: U-2 OS cell lysate (15 µg/Lane)<br />Lane 6: Jurkat cell lysate (15 µg/Lane)<br />Lane 7: Neuro-2a cell lysate (15 µg/Lane)<br />Lane 8: NIH/3T3 cell lysate (15 µg/Lane)<br />Lane 9: PC-12 cell lysate (15 µg/Lane)<br />Lane 10: Mouse brain tissue lysate (20 µg/Lane)<br />Lane 11: Rat brain tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 50 kDa<br />Observed band size: 50 kDa<br /><br />Exposure time: 1 minute 20 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>) at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of beta Tubulin on different lysates with Rabbit anti-beta Tubulin antibody (ET1602-4) at 1/20,000 dilution.

    Lane 1: HeLa cell lysate (15 µg/Lane)
    Lane 2: 293T cell lysate (15 µg/Lane)
    Lane 3: MCF7 cell lysate (15 µg/Lane)
    Lane 4: SH-SY5Y cell lysate (15 µg/Lane)
    Lane 5: U-2 OS cell lysate (15 µg/Lane)
    Lane 6: Jurkat cell lysate (15 µg/Lane)
    Lane 7: Neuro-2a cell lysate (15 µg/Lane)
    Lane 8: NIH/3T3 cell lysate (15 µg/Lane)
    Lane 9: PC-12 cell lysate (15 µg/Lane)
    Lane 10: Mouse brain tissue lysate (20 µg/Lane)
    Lane 11: Rat brain tissue lysate (20 µg/Lane)

    Predicted band size: 50 kDa
    Observed band size: 50 kDa

    Exposure time: 1 minute 20 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-4) at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of SH-SY5Y cells labeling beta Tubulin with Rabbit anti-beta Tubulin antibody (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>) at 1/200 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-beta Tubulin antibody (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of SH-SY5Y cells labeling beta Tubulin with Rabbit anti-beta Tubulin antibody (ET1602-4) at 1/200 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-beta Tubulin antibody (ET1602-4) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Immunocytochemistry analysis of Hela cells labeling beta Tubulin with Rabbit anti-beta Tubulin antibody (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>) at 1/200 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-beta Tubulin antibody (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 594, <a href="/products/HA1122" style="font-weight: bold;text-decoration: underline;">HA1122</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of Hela cells labeling beta Tubulin with Rabbit anti-beta Tubulin antibody (ET1602-4) at 1/200 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-beta Tubulin antibody (ET1602-4) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

  • Immunocytochemistry analysis of NIH/3T3 cells labeling beta Tubulin with Rabbit anti-beta Tubulin antibody (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>) at 1/200 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-beta Tubulin antibody (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 594, <a href="/products/HA1122" style="font-weight: bold;text-decoration: underline;">HA1122</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of NIH/3T3 cells labeling beta Tubulin with Rabbit anti-beta Tubulin antibody (ET1602-4) at 1/200 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-beta Tubulin antibody (ET1602-4) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

  • Immunocytochemistry analysis of PC-12 cells labeling beta Tubulin with Rabbit anti-beta Tubulin antibody (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-beta Tubulin antibody (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of PC-12 cells labeling beta Tubulin with Rabbit anti-beta Tubulin antibody (ET1602-4) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-beta Tubulin antibody (ET1602-4) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-beta Tubulin antibody (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-beta Tubulin antibody (ET1602-4) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-4) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-beta Tubulin antibody (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-beta Tubulin antibody (ET1602-4) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-4) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-beta Tubulin antibody (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-beta Tubulin antibody (ET1602-4) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-4) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of NIH/3T3 cells labeling beta Tubulin.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of NIH/3T3 cells labeling beta Tubulin.

    Cells were fixed and permeabilized. Then stained with the primary antibody (ET1602-4, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

ご注意ください: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

論文での実績

Filter:
  1. WB
  2. IF-tissue
  3. IF
  4. IHC

同じターゲット & 同じ経路の製品

metafield image

beta Tubulin Mouse Monoclonal Antibody [1-B11]

Application: WB,IF-Cell,IHC-P,FC,IF-Tissue

Reactivity: Human,Mouse,Rat,Zebrafish

Conjugate: unconjugated

metafield image

HRP Conjugated beta Tubulin Mouse Monoclonal Antibody [1-B11]

Application: WB,IHC-P

Reactivity: Human,Mouse,Rat,Zebrafish

Conjugate: HRP

metafield image

iFluor™ 594 Conjugated Beta tubulin Recombinant Mouse Monoclonal Antibody [A1-A4-R]

Application: IF-Cell,IF-Tissue,FC

Reactivity: Human,Mouse

Conjugate: iFluor™ 594

metafield image

beta Tubulin Recombinant Mouse Monoclonal Antibody [1-B11-R]

Application: WB,IF-Cell,IHC-P

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

HRP Conjugated beta Tubulin Recombinant Rabbit Monoclonal Antibody [JF41-50]

Application: WB

Reactivity: Human,Mouse,Rat

Conjugate: HRP

metafield image

beta Tubulin Recombinant Mouse Monoclonal Antibody [A1-A4-R]

Application: WB,IF-Cell,FC,IHC-P

Reactivity: Human,Mouse,Rat,Monkey

Conjugate: unconjugated

metafield image

beta Tubulin Mouse Monoclonal Antibody [A1-A4]

Application: WB,IF-Cell,IHC-P,FC

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

Close (esc)

Popup

Use this popup to embed a mailing list sign up form. Alternatively use it as a simple call to action with a link to a product or a page.

Age verification

By clicking enter you are verifying that you are old enough to consume alcohol.

Enter

搜索

主菜单