DNAJC5 Recombinant Rabbit Monoclonal Antibody [PSH24-38]
Catalog# HA724445
DNAJC5 Recombinant Rabbit Monoclonal Antibody [PSH24-38]
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WB
-
IF-Cell
-
IHC-Fr
-
IHC-P
-
FC(Intra)
-
IP
-
Human
-
Mouse
-
Rat
-
Green monkey
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Pig
-
HA751939
不含抗保成分
-
unconjugated
概要
製品名
DNAJC5 Recombinant Rabbit Monoclonal Antibody [PSH24-38]
抗体の種類
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human DNAJC5 aa 151-198.
種属反応性
Human, Mouse, Rat, Green monkey, Pig
検証された応用例
WB, IF-Cell, IHC-Fr, IHC-P, FC(Intra), IP
分子量
Predicted band size: 22 kDa
陽性対照
HeLa (Human cervical adenocarcinoma cells) cell lysate, THP-1 (Human acute monoblastic leukemia cells) cell lysate, HepG2 (Human liver cancer cells) cell lysate, Huh7 (Human liver cancer cells) cell lysate, NIH/3T3 (Mouse fibroblasts) cell lysate, RAW264.7 (Mouse monocytic macrophage leukemia cells) cell lysate, PC-12 (Rat pheochromocytoma cells (undifferentiated)) cell lysate, C6 (Rat glioma cells) cell lysate, COS-1 (African green monkey kidney fibroblasts) cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, Pig brain tissue lysate.
結合
unconjugated
クローン番号
PSH24-38
製品の特徴
形態
Liquid
濃度
保存方法
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
保存用バッファー
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
アイソタイプ
IgG
精製方法
Protein A affinity purified.
応用希釈度
-
WB
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1:5,000
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IF-Cell
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1:100
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IHC-Fr
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1:500
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IHC-P
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1:200-1:1,000
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FC(Intra)
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1:1,000
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IP
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1-2μg/sample
ターゲット
機能
DnaJ homolog subfamily C member 5, also known as cysteine string protein or CSP is a protein, that in humans encoded by the DNAJC5 gene. It was first described in 1990. In humans, the gene is located on the long arm of chromosome 20 (20q13.33) on the Watson (positive strand). The gene is 40,867 bases in length and the encoded protein has 198 amino acids with a predicted molecular weight of 22.149 kilodaltons (kDa). The weight of the mature protein is 34 kDa. This gene is highly conserved and found both in invertebrates and vertebrates. In humans, a pseudogene of this gene is located on the short arm of chromosome 8.
背景文献
1. Lee J et al. Safeguarding Lysosomal Homeostasis by DNAJC5/CSPalpha-Mediated Unconventional Protein Secretion and Endosomal Microautophagy. Front Cell Dev Biol. 2022 May
2. Chen C et al. DNAJC5 facilitates the proliferation and migration of lung adenocarcinoma cells by augmenting EGFR trafficking. Commun Biol. 2025 May
亜細胞局在
Cytoplasm, cytosol, Membrane, Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane , Melanosome, Cell membrane.
別名
CLN 4 antibody
CLN4 antibody
CLN4B antibody
CSP antibody
cysteine string protein alpha antibody
Cysteine string protein antibody
DJC5 antibody
DnaJ (Hsp40) homolog subfamily C member 5 antibody
DnaJ homolog subfamily C member 5 antibody
DNAJC 5 antibody
展開画像
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Western blot analysis of DNAJC5 on different lysates with Rabbit anti-DNAJC5 antibody (HA724445) at 1/5,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cells) cell lysate
Lane 2: THP-1 (Human acute monoblastic leukemia cells) cell lysate
Lane 3: HepG2 (Human liver cancer cells) cell lysate
Lane 4: Huh7 (Human liver cancer cells) cell lysate
Lane 5: NIH/3T3 (Mouse fibroblasts) cell lysate
Lane 6: RAW264.7 (Mouse monocytic macrophage leukemia cells) cell lysate
Lane 7: PC-12 (Rat pheochromocytoma cells (undifferentiated)) cell lysate
Lane 8: C6 (Rat glioma cells) cell lysate
Lane 9: COS-1 (African green monkey kidney fibroblasts) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 7 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724445, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 22 kDa
Observed band size: 34 kDa -
Application: Immunocytochemistry (IF-cell)
Species: Rat
Sample: PC-12 (Rat pheochromocytoma cells (undifferentiated))
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA724445, 1/100, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue). -
☑ Relative expression (RE)
Western blot analysis of DNAJC5 on different lysates with Rabbit anti-DNAJC5 antibody (HA724445) at 1/5,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Mouse kidney tissue lysate
Lane 3: Rat brain tissue lysate
Lane 4: Rat kidney tissue lysate
Lane 5: Pig brain tissue lysate
Lysates/proteins at 30 µg/Lane.
Exposure time: 7 seconds; ECL: K1801
Negative expression of DNAJC5 protein in kidney tissue is consistent with the predicted expression pattern.
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724445, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 22 kDa
Observed band size: 34 kDa -
Application: Immunofluorescence (IHC-Fr)
Species: Rat
Tissue: Brain
Sample: Frozen section
Antigen retrieval: Not required
Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA724445, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. -
Application: Immunofluorescence (IHC-Fr)
Species: Mouse
Tissue: Brain
Sample: Frozen section
Antigen retrieval: Not required
Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA724445, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Placenta
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724445, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Brain
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724445, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Lung
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724445, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Placenta
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724445, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Brain
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724445, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Lung
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724445, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Placenta
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724445, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Brain
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724445, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Lung
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724445, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Flow Cytometry (Intra)
Species: Human
Sample: HeLa (Human cervix adenocarcinoma epithelial cell)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA724445 (1/1,000) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. -
Application: Flow Cytometry (Intra)
Species: Mouse
Sample: NIH/3T3 (Mouse fibroblasts)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA724445 (1/1,000) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. -
Application: Flow Cytometry (Intra)
Species: Rat
Sample: PC-12 (Rat pheochromocytoma cells (undifferentiated))
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA724445 (1/1,000) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. -
Immunoprecipitation (IP)
DNAJC5 was immunoprecipitated in 0.2 mg HeLa (Human cervix adenocarcinoma epithelial cell) cell lysate with HA724445 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724445 at 1/25,000 dilution. Alpaca anti-Rabbit IgG Fc, Recombinant VHH monoclonal Antibody(HA1031) at 1/50000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (input)
Lane 2: HA724445 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of HA724445 in HeLa cell lysate
Exposure time: 2 seconds
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary dilution: HA724445, 1/25,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature
Predicted band size: 22 kDa
Observed band size: 34 kDa
ご注意ください: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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