MLH1 Recombinant Antibody [PSH20-67] - Rabbit IgG (Chimeric)
Catalog# HA724342
MLH1 Recombinant Antibody [PSH20-67] - Rabbit IgG (Chimeric)
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IHC-P
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IF-Cell
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WB
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Human
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unconjugated
製品概要
製品名
MLH1 Recombinant Antibody [PSH20-67] - Rabbit IgG (Chimeric)
抗体のタイプ
Recombinant Chimeric Antibody
交差反応性（対応種属）
Human
検証済みアプリケーション
IHC-P, IF-Cell, WB
標的分子量
Predicted band size: 85 kDa
ポジティブコントロール
HeLa (Human cervical adenocarcinoma cells) cell lysate, SW480 (Human colorectal zdenocarcinoma cells) cell lysate.
コンジュゲーション
unconjugated
クローン番号
PSH20-67
製品の特徴
形態
Liquid
濃度
保存方法
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
保存バッファー
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
精製方法
Protein A affinity purified.
推奨希釈倍率
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IHC-P
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1:2,000
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IF-Cell
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1:3,000
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WB
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1:2,000
ターゲット
機能
Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.
背景・参考文献
1. Yue Q et al. Histone H3K9 Lactylation Confers Temozolomide Resistance in Glioblastoma via LUC7L2-Mediated MLH1 Intron Retention. Adv Sci (Weinh). 2024 May
2. Guan J et al. MLH1 Deficiency-Triggered DNA Hyperexcision by Exonuclease 1 Activates the cGAS-STING Pathway. Cancer Cell. 2021 Jan
サブセルラー局在
Nucleus, Chromosome.
UNIPROT #
別名
COCA 2 antibody
COCA2 antibody
DNA mismatch repair protein Mlh1 antibody
FCC 2 antibody
FCC2 antibody
hMLH 1 antibody
hMLH1 antibody
HNPCC 2 antibody
HNPCC antibody
HNPCC2 antibody
詳細を見る画像
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Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Tonsil
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0) (high pressure), 2 minutes.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724342, 1/2,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Appendix
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0) (high pressure), 2 minutes.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724342, 1/2,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
☑ Relative expression (RE)
Application: Immunocytochemistry (IF-cell)
Species: Human
Sample: SW480 (Human colorectal zdenocarcinoma cells)/HCT 116 (Human colon cancer cells)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA724342, 1/3,000, overnight at 4℃.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue).
Negative control: HCT 116 (PMID: 27618981). -
☑ Relative expression (RE)
Western blot analysis of MLH1 on different lysates with Rabbit anti-MLH1 antibody (HA724342) at 1/2,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cells) cell lysate
Lane 2: HCT 116 (Human colon cancer cells) cell lysate (negative)
Lane 3: SW480 (Human colorectal zdenocarcinoma cells) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 12 seconds; ECL: K1801
HCT 116 is a negative control (PMID: 34787019).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724342, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 85 kDa
Observed band size: 85 kDa
ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）