HRP Conjugated Human CD163 Recombinant Rabbit Monoclonal Antibody [PSH13-79] - Detector
製品概要
製品名
HRP Conjugated Human CD163 Recombinant Rabbit Monoclonal Antibody [PSH13-79] - Detector
抗体のタイプ
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human CD163 aa 42-1,050 (HA211143).
交差反応性（対応種属）
Human
検証済みアプリケーション
ELISA(Det), ELISA
ポジティブコントロール
Recombinant Human CD163 protein (HA211143).
コンジュゲーション
HRP
クローン番号
PSH13-79
製品の特徴
形態
Liquid
濃度
保存方法
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
保存バッファー
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
アイソタイプ
IgG
精製方法
Protein A affinity purified.
推奨希釈倍率
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH13-78] to Human CD163 antibody (Capture) (HA723566) or Rabbit monoclonal [PSH13-80] to Human CD163 antibody (Capture) (HA723569) and recombinant Human CD163 protein (HA211143) as the standard. The reference range value is 78.1-10,000 pg/mL.
-
ELISA
-
Use at an assay dependent concentration.
ターゲット
機能
CD163, also designated M130, is a macrophage-associated antigen that is a member of the scavenger receptor cysteine-rich (SRCR) superfamily. It is highly expressed on macrogphages and to a lesser extent on monocytes. The acute phase-regulated and signal-inducing macrophage protein, CD163, is a receptor that scavenges hemoglobin by mediating endocytosis of haptoglobin-hemoglobin complexes. CD163 binds only haptoglobin and hemoglobin in complex, which indicates the exposure of a receptor-binding neoepitope. The receptor-ligand interaction is calcium-dependent and of high affinity. The existence of several CD163 isoforms, which differ in the structure of their cytoplasmic domains and putative phosphorylation sites, suggests that these isoforms also differ in their signaling mechanism. The gene which encodes CD163 maps to human chromosome 12p13.31.
背景・参考文献
1. Mori M et al. CD163(+) Macrophages Induce Endothelial-to-Mesenchymal Transition in Atheroma. Circ Res. 2024 Jul
2. Rowland RRR et al. Role of CD163 in PRRSV infection. Virology. 2024 Dec
サブセルラー局在
Cell membrane; Secreted.
UNIPROT #
別名
C163A_HUMAN antibody
CD 163 antibody
CD163 antibody
CD163 antigen antibody
CD163 molecule antibody
Hemoglobin scavenger receptor antibody
M130 antibody
M130 antigen precursor antibody
Macrophage associated antigen antibody
MM130 antibody
詳細を見る画像
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![Sandwich ELISA analysis of human CD163 matched pair antibodies<br /><br />Capture: <a href="/products/HA723566" style="font-weight: bold;text-decoration: underline;">HA723566</a>, Human CD163 Rabbit mAb [PSH13-78]<br />Detector: <a href="/products/HA723567" style="font-weight: bold;text-decoration: underline;">HA723567</a>, Human CD163 Rabbit mAb [PSH13-79]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA723566" style="font-weight: bold;text-decoration: underline;">HA723566</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human CD163 protein (<a href="/products/HA211143" style="font-weight: bold;text-decoration: underline;">HA211143</a>) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723567" style="font-weight: bold;text-decoration: underline;">HA723567</a>, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA723568H_1.jpg?v=20260805152209)
Sandwich ELISA analysis of human CD163 matched pair antibodies
Capture: HA723566, Human CD163 Rabbit mAb [PSH13-78]
Detector: HA723567, Human CD163 Rabbit mAb [PSH13-79]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723566) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human CD163 protein (HA211143) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA723567, HRP, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）
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