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CD163 Recombinant Antibody [JA51-30] - Rat IgG1 (Chimeric)

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Catalog# HA601549

CD163 Recombinant Antibody [JA51-30] - Rat IgG1 (Chimeric)

Application

  • IHC-P

  • mIHC

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

製品概要

製品名

CD163 Recombinant Antibody [JA51-30] - Rat IgG1 (Chimeric)

抗体のタイプ

Recombinant Chimeric Antibody

免疫原

Recombinant protein within Human CD163 aa 1012-1149 / 1156.

交差反応性（対応種属）

Human

検証済みアプリケーション

IHC-P, mIHC

標的分子量

Predicted band size: 125 kDa

ポジティブコントロール

Human liver tissue, human placenta tissue, human spleen tissue, human tonsil tissue.

コンジュゲーション

unconjugated

クローン番号

JA51-30

Reactivity Data

IHC-P mIHC
Human
Tested Tested
Mouse
Rat

製品の特徴

形態

Liquid

濃度

保存方法

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

保存バッファー

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

精製方法

Protein A affinity purified.

推奨希釈倍率

  • IHC-P

  • 1:1,000

  • mIHC

  • 1:200

ターゲット

機能

CD163, also designated M130, is a macrophage-associated antigen that is a member of the scavenger receptor cysteine-rich (SRCR) superfamily. It is highly expressed on macrogphages and to a lesser extent on monocytes. The acute phase-regulated and signal-inducing macrophage protein, CD163, is a receptor that scavenges hemoglobin by mediating endocytosis of haptoglobin-hemoglobin complexes. CD163 binds only haptoglobin and hemoglobin in complex, which indicates the exposure of a receptor-binding neoepitope. The receptor-ligand interaction is calcium-dependent and of high affinity. The existence of several CD163 isoforms, which differ in the structure of their cytoplasmic domains and putative phosphorylation sites, suggests that these isoforms also differ in their signaling mechanism. The gene which encodes CD163 maps to human chromosome 12p13.31.

背景・参考文献

1. Sato Y et al. The PD-1/PD-L1 axis may be aberrantly activated in occupational cholangiocarcinoma.Pathol Int 67(3):163-170 (2017).

2. Chen H et al. An Agonist of the Protective Factor SIRT1 Improves Functional Recovery and Promotes Neuronal Survival by Attenuating Inflammation after Spinal Cord Injury. J Neurosci 37:2916-2930 (2017).

サブセルラー局在

Cell membrane; Secreted.

UNIPROT #

SwissProt: Q86VB7 Human

別名

C163A_HUMAN antibody

CD 163 antibody

CD163 antibody

CD163 antigen antibody

CD163 molecule antibody

Hemoglobin scavenger receptor antibody

M130 antibody

M130 antigen precursor antibody

Macrophage associated antigen antibody

MM130 antibody

詳細を見る

画像

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rat anti-CD163 antibody (<a href="/products/HA601549" style="font-weight: bold;text-decoration: underline;">HA601549</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601549" style="font-weight: bold;text-decoration: underline;">HA601549</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rat anti-CD163 antibody (HA601549) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601549) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rat anti-CD163 antibody (<a href="/products/HA601549" style="font-weight: bold;text-decoration: underline;">HA601549</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601549" style="font-weight: bold;text-decoration: underline;">HA601549</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rat anti-CD163 antibody (HA601549) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601549) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rat anti-CD163 antibody (<a href="/products/HA601549" style="font-weight: bold;text-decoration: underline;">HA601549</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601549" style="font-weight: bold;text-decoration: underline;">HA601549</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rat anti-CD163 antibody (HA601549) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601549) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rat anti-CD163 antibody (<a href="/products/HA601549" style="font-weight: bold;text-decoration: underline;">HA601549</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601549" style="font-weight: bold;text-decoration: underline;">HA601549</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rat anti-CD163 antibody (HA601549) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601549) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）

文献・引用論文

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