Phospho-Lamin A + Lamin C (S22) Recombinant Rabbit Monoclonal Antibody [PSH11-48]
概要
製品名
Phospho-Lamin A + Lamin C (S22) Recombinant Rabbit Monoclonal Antibody [PSH11-48]
抗体の種類
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic phospho-peptide corresponding to residues surrounding Ser22 of Human Lamin A + Lamin C.
種属反応性
Human, Mouse, Rat
検証された応用例
WB
分子量
Predicted band size: 74 kDa
陽性対照
HeLa cell lysate, HeLa treated with 100nM paclitaxel for 20 hours cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 100nM paclitaxel for 20 hours cell lysate, C6 cell lysate, C6 treated with 4mM hydroxyurea for 20 hours cell lysate.
結合
unconjugated
クローン番号
PSH11-48
製品の特徴
形態
Liquid
保存方法
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
保存用バッファー
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
アイソタイプ
IgG
精製方法
Protein A affinity purified.
応用希釈度
-
WB
-
1:2,000
ターゲット
機能
Prelamin-A/C, or lamin A/C is a protein that in humans is encoded by the LMNA gene. Lamin A/C belongs to the lamin family of proteins. DNA double-strand damages can be repaired by either homologous recombination (HR) or non-homologous end joining (NHEJ). LMNA promotes genetic stability by maintaining the levels of proteins that have key roles in HR and NHEJ. Mouse cells that are deficient for maturation of prelamin A have increased DNA damage and chromosome aberrations, and show increased sensitivity to DNA damaging agents. In progeria, the inadequacy of DNA repair, due to defective LMNA, may cause features of premature aging (see DNA damage theory of aging).
背景文献
1. Kovacs MT et. al. DNA damage induces nuclear envelope rupture through ATR-mediated phosphorylation of lamin A/C. Mol Cell. 2023 Oct
2. Yamada S et al. TEAD1 trapping by the Q353R-Lamin A/C causes dilated cardiomyopathy. Sci Adv. 2023 Apr
亜細胞局在
Nucleus lamina, Nucleus envelope, nucleoplasm, Nucleus matrix; Nucleus speckle.
別名
70 kDa lamin antibody
Cardiomyopathy dilated 1A (autosomal dominant) antibody
CDCD1 antibody
CDDC antibody
CMD1A antibody
CMT2B1 antibody
EMD2 antibody
FPL antibody
FPLD antibody
FPLD2 antibody
展開画像
-
☑ Cell treatment (CT)
Western blot analysis of Phospho-Lamin A + Lamin C (S22) on different lysates with Rabbit anti-Phospho-Lamin A + Lamin C (S22) antibody (HA723340) at 1/2,000 dilution and pan Lamin A + Lamin C antibody (ET7110-12) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 100nM paclitaxel for 20 hours cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: NIH/3T3 treated with 100nM paclitaxel for 20 hours cell lysate
Lane 5: C6 cell lysate
Lane 6: C6 treated with 4mM hydroxyurea for 20 hours cell lysate
Lane 7: HeLa treated with 100nM paclitaxel for 20 hours cell lysate, then the membrane treated with λpp for 1 hour
Lane 8: NIH/3T3 treated with 100nM paclitaxel for 20 hours cell lysate, then the membrane treated with λpp for 1 hour
Lane 9: C6 treated with 4mM hydroxyurea for 20 hours cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 74 kDa
Observed band size: 70/65 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723340) at 1/2,000 dilution and pan Lamin A + Lamin C antibody (ET7110-12) at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ご注意ください: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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