Phospho-Lamin A + Lamin C (S22) Recombinant Rabbit Monoclonal Antibody [PSH11-48] - BSA and Azide free

☑ Cell treatment (CT)

This data was developed using HA723340, the same antibody clone in a different buffer formulation.
Western blot analysis of Phospho-Lamin A + Lamin C (S22) on different lysates with Rabbit anti-Phospho-Lamin A + Lamin C (S22) antibody (HA723340) at 1/2,000 dilution and pan Lamin A + Lamin C antibody (ET7110-12) at 1/2,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 100nM paclitaxel for 20 hours cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: NIH/3T3 treated with 100nM paclitaxel for 20 hours cell lysate
Lane 5: C6 cell lysate
Lane 6: C6 treated with 4mM hydroxyurea for 20 hours cell lysate
Lane 7: HeLa treated with 100nM paclitaxel for 20 hours cell lysate, then the membrane treated with λpp for 1 hour
Lane 8: NIH/3T3 treated with 100nM paclitaxel for 20 hours cell lysate, then the membrane treated with λpp for 1 hour
Lane 9: C6 treated with 4mM hydroxyurea for 20 hours cell lysate, then the membrane treated with λpp for 1 hour

Lysates/proteins at 20 µg/Lane.

Predicted band size: 74 kDa
Observed band size: 70/65 kDa

Exposure time: 4 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723340) at 1/2,000 dilution and pan Lamin A + Lamin C antibody (ET7110-12) at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.