Phospho-NF-kB p65 (S536) Recombinant Rabbit Monoclonal Antibody [PSH10-58]
製品概要
製品名
Phospho-NF-kB p65 (S536) Recombinant Rabbit Monoclonal Antibody [PSH10-58]
抗体のタイプ
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic phospho-peptide corresponding to residues surrounding Ser536 of Human NF-kB p65.
交差反応性（対応種属）
Human, Mouse, Rat
検証済みアプリケーション
WB, IF-Cell
標的分子量
Predicted band size: 60 kDa
ポジティブコントロール
HeLa treated with 100nM Calyculin A and 20ng/mL TNF-α for 10 minutes cell lysate, NIH/3T3 treated with 100nM Calyculin A and 20ng/mL TNF-α for 10 minutes cell lysate, HeLa cells treated with 100nM Calyculin A and 20ng/mL TNF-α for 15 minutes, C6 treated with 100ng/mL Calyculin A for 1 hour cell lysate, PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate.
コンジュゲーション
unconjugated
クローン番号
PSH10-58
Reactivity Data
| WB | IF-Cell | |
|---|---|---|
| Human |
|
|
| Mouse |
|
|
| Rat |
|
|
| Yak |
|
製品の特徴
形態
Liquid
濃度
保存方法
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
保存バッファー
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
アイソタイプ
IgG
精製方法
Protein A affinity purified.
推奨希釈倍率
-
WB
-
1:5,000
-
IF-Cell
-
1:500
ターゲット
機能
Transcription factor p65 also known as nuclear factor NF-kappa-B p65 subunit is a protein that in humans is encoded by the RELA gene. RELA, also known as p65, is a REL-associated protein involved in NF-κB heterodimer formation, nuclear translocation and activation. NF-κB is an essential transcription factor complex involved in all types of cellular processes, including cellular metabolism, chemotaxis, etc. Phosphorylation and acetylation of RELA are crucial post-translational modifications required for NF-κB activation. RELA has also been shown to modulate immune responses, and activation of RELA is positively associated with multiple types of cancer.
背景・参考文献
1. Shim H et al. NF-kappaB p65 represses microRNA-124 transcription in diffuse large B-cell lymphoma. Genes Genomics. 2020 May
2. Ren C et al. Ubiquitination of NF-kappaB p65 by FBXW2 suppresses breast cancer stemness, tumorigenesis, and paclitaxel resistance. Cell Death Differ. 2022 Feb
サブセルラー局在
Nucleus, Cytoplasm.
別名
Avian reticuloendotheliosis viral (v rel) oncogene homolog A antibody
MGC131774 antibody
NF kappa B p65delta3 antibody
nfkappabp65 antibody
NFkB p65 antibody
NFKB3 antibody
Nuclear factor kappaB antibody
Nuclear Factor NF Kappa B p65 Subunit antibody
Nuclear factor NF-kappa-B p65 subunit antibody
Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 antibody
詳細を見る画像
-
☑ Cell treatment (CT)
Western blot analysis of Phospho-NF-kB p65 (S536) on different lysates with Rabbit anti-Phospho-NF-kB p65 (S536) antibody (HA723223) at 1/5,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HeLa treated with 100nM Calyculin A and 20ng/mL TNF-α for 10 minutes cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
Lane 4: NIH/3T3 treated with 100nM Calyculin A and 20ng/mL TNF-α for 10 minutes cell lysate (20 µg/Lane)
Predicted band size: 60 kDa
Observed band size: 65 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723223) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Immunocytochemistry analysis of HeLa cells untreated / treated with 100nM Calyculin A and 20ng/mL TNF-α for 15 minutes labeling Phospho-NF-kB p65 (S536) with Rabbit anti-Phospho-NF-kB p65 (S536) antibody (HA723223) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-NF-kB p65 (S536) antibody (HA723223) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Western blot analysis of Phospho-NF-kB p65 (S536) on different lysates with Rabbit anti-Phospho-NF-kB p65 (S536) antibody (HA723223) at 1/5,000 dilution.
Lane 1: C6 cell lysate (20 µg/Lane)
Lane 2: C6 treated with 100ng/mL Calyculin A for 1 hour cell lysate (20 µg/Lane)
Lane 3: PC-12 cell lysate (20 µg/Lane)
Lane 4: PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate (20 µg/Lane)
Predicted band size: 60 kDa
Observed band size: 65 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723223) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）
文献・引用論文
-
Liquiritigenin Ameliorates Rheumatoid Arthritis by Modulating the Nrf2/NF-κB/NLRP3 Pathway in Fibroblast-like Synoviocytes
ジャーナル: Pharmaceuticals
DOI: 10.3390/ph19050785
IF: 5.7
アプリケーション: WB
交差反応性: Rat
掲載日: 2026 May
-
Aryl hydrocarbon receptor alleviates intestinal ischemia/reperfusion-induced epithelial barrier damage by inhibiting the 5-hydroxytryptamine-mediated complement C3 cascade
ジャーナル: International Journal Of Biological Macromolecules
DOI: 10.1016/j.ijbiomac.2026.152765
IF: 8.7
アプリケーション: WB
交差反応性: Human
掲載日: 2026 May
-
Time-sequentially released bilayer sandwich cerium oxide nanoparticles on femtosecond laser-treated zirconia implant surface for enhanced antibacterial and osseointegration capacity
ジャーナル: Materials Today Bio
DOI: 10.1016/j.mtbio.2026.103206
IF: 11
アプリケーション: WB
交差反応性: Mouse
掲載日: 2026 May
-
Caffeic Acid Counteracts LPS-Induced Inflammatory Damage in Yak Mammary Epithelial Cells Associated with NF-κB-Mediated Autophagy Regulation
ジャーナル: Animals
DOI: 10.3390/ani16111605
IF: 3.2
アプリケーション: WB
交差反応性: yak
掲載日: 2026 May
-
The hypouricemic and renal protective effects of the Chinese functional tea Ligustrum robustum on hyperuricemic mice
ジャーナル: Food & Function
DOI: 10.1039/D6FO00994H
IF: 6.3
アプリケーション: WB
交差反応性: Mouse
掲載日: 2026 May
-
Shengxian Decoction mitigate bleomycin-induced pulmonary fibrosis in mice via MerTK mediated macrophage efferocytosis
ジャーナル: Journal Of Ethnopharmacology
DOI: 10.1016/j.jep.2026.121583
IF: 5.4
アプリケーション: WB
交差反応性: Mouse
掲載日: 2026 Mar
-
Celastrol Mitigates Acute Pancreatitis Associated Inflammation by Modulating the IL-34/CSF-1R Axis and Suppressing NF-κB/ERK Signaling
ジャーナル: Journal Of Inflammation Research
DOI: 10.2147/JIR.S572609
IF: 4.1
アプリケーション: WB
交差反応性: Rat
掲載日: 2026 Mar
-
Titanium-Guided Neutrophil Dynamics for Inflammation Resolution and Tissue Integration
ジャーナル: Biomaterials
DOI: 10.1016/j.biomaterials.2026.124048
IF: 12.9
アプリケーション: WB
交差反応性: Mouse
掲載日: 2026 Feb
-
Necroptosis mediated by FKBP5 in alveolar fibroblasts drives Pseudomonas aeruginosa-induced acute lung injury
ジャーナル: International Immunopharmacology
DOI: 10.1016/j.intimp.2026.116368
IF: 4.7
アプリケーション: WB
交差反応性: Mouse,Human
掲載日: 2026 Feb
-
From Multi-omics Discovery to Experimental Validation: GPC4 Mediates the Anti-neuroinflammatory Effects of Buyang Huanwu Decoction via TLR4/NF-κB Signaling in Ischemic Stroke with Qi Deficiency and Blood Stasis Syndrome
ジャーナル: Journal Of Ethnopharmacology
DOI: 10.1016/j.jep.2026.121375
IF: 5.4
アプリケーション: WB
交差反応性: Rat
掲載日: 2026 Feb
-
Platycodin D ameliorates antibiotic-associated diarrhea by modulating the PI3K/AKT/NF-κB pathway and regulating gut microbiota and metabolism
ジャーナル: International Immunopharmacology
DOI: 10.1016/j.intimp.2026.116748
IF: 4.7
アプリケーション: WB
交差反応性: Mouse
掲載日: 2026 Apr
-
Asthmatic Pulmonary Fibroblasts Aggravate Airway Inflammation Through FKBP5-Dependent Necroptosis
ジャーナル: American Journal of Respiratory Cell and Molecular Biology
DOI: 10.1093/ajrcmb/aanag080
IF: 5.3
アプリケーション: WB
交差反応性: Mouse
掲載日: 2026 Apr
-
A dihydrofuro[2,3-b] benzofuran derivative alleviates lipopolysaccharide induced acute lung injury via suppressing MAPK signaling
ジャーナル: Frontiers In Pharmacology
DOI: 10.3389/fphar.2026.1763318
IF: 4.8
アプリケーション: WB
交差反応性: Mouse
掲載日: 2026 Apr
-
Atractylenolide III Alleviates Inflammation in Cerebral Ischemia/Reperfusion Injury by Modulating the PI3K/Akt/NF-κB Signaling Pathway
ジャーナル: Journal Of Ethnopharmacology
DOI: 10.1016/j.jep.2025.120644
IF: 5.4
アプリケーション: WB
交差反応性: Rat
掲載日: 2025 Sept
-
IKK/NF-κB Inactivation by Salidroside via Targeting TNF-α for the Treatment of LPS-Induced Colitis
ジャーナル: Current Issues In Molecular Biology
DOI: 10.3390/cimb47110896
IF: 3
アプリケーション: WB
交差反応性: Human,Mouse
掲載日: 2025 Oct
-
Engineered self-assembly of plant proteins into α-ketoglutarate-loaded nanoplatforms for osteoarthritis treatment
ジャーナル: Journal Of Controlled Release
DOI: 10.1016/j.jconrel.2025.114326
IF: 11.5
アプリケーション: WB
交差反応性: Mouse
掲載日: 2025 Oct
-
Atractylenolide III Ameliorates Ulcerative Colitis By Targeting IL-17RA to Suppress Macrophage M1 Polarization
ジャーナル: Journal Of Agricultural And Food Chemistry
DOI: 10.1021/acs.jafc.5c09397
IF: 6.2
アプリケーション: WB
交差反応性: Mouse
掲載日: 2025 Nov
-
The Single and Combined Effects of Decabromodiphenyl Ethane and Mixed Microplastics on Male Mice Reproductive Toxicity
ジャーナル: Biology Of Reproduction
DOI: 10.1093/biolre/ioaf121
IF: 3.1
アプリケーション: WB
交差反応性: Mouse
掲載日: 2025 May
-
KK2DP7 Stimulates CD11b+ Cell Populations in the Spleen to Elicit Trained Immunity for Anti-Tumor Therapy
ジャーナル: Advanced Science
DOI: 10.1002/advs.202500032
IF: 14.3
アプリケーション: WB
交差反応性: Mouse
掲載日: 2025 Mar
-
FKBP5 Mediates Alveolar Fibroblast Necroptosis During Acute Respiratory Distress Syndrome
ジャーナル: Cell Proliferation
DOI: 10.1111/cpr.70075
IF: 5.6
アプリケーション: WB
交差反応性: Mouse,Human
掲載日: 2025 Jun
-
Targeting DKC1/NF-κB axis suppresses tumorigenesis and enhances 5-FU sensitivity in gastric cancer
ジャーナル: Cancer Cell International
DOI: 10.1186/s12935-025-03926-4
IF: 6
アプリケーション: WB
交差反応性: Human
掲載日: 2025 Jul
-
Anti-Inflammatory Effect of 21α-Methylmelianol In Vitro and In Vivo via NF-κB/STAT Signaling Pathway Modulation
ジャーナル: Food Science & Nutrition
DOI: 10.1002/fsn3.71310
IF: 3.8
アプリケーション: WB
交差反応性:
掲載日: 2025 Dec
-
A strategy for challenging tumorous bone regeneration by borosilicate bioactive glass boosting moderate magnetic hyperthermia
ジャーナル: Nature Communications
DOI: 10.1038/s41467-025-63270-z
IF: 15.7
アプリケーション: WB
交差反応性: Human
掲載日: 2025 Aug
-
Highly Soluble Mussel Foot Protein and Its Derivatives Inhibit Inflammation by Targeting NF-κB/PI3K-Akt Signaling and Promoting M2 Macrophage Polarization
ジャーナル: Antioxidants
DOI: 10.3390/antiox14081021
IF: 6.6
アプリケーション: WB
交差反応性: Mouse
掲載日: 2025 Aug
-
TNFSF14‐HVEM/LTβR Exacerbates Keratinocyte Abnormalities and IMQ‐Induced Psoriatic Skin Inflammation via Activating NF‐κB/TWIST1 Signalling Pathway
ジャーナル: Journal Of Cellular And Molecular Medicine
DOI: 10.1111/jcmm.70774
IF: 4.2
アプリケーション: WB
交差反応性: Mouse,Human
掲載日: 2025 Aug
-
Tβ4-Engineered ADSC Extracellular Vesicles Rescue Cell Senescence Through Separable Microneedle Patches for Diabetic Wound Healing
ジャーナル:
DOI: 10.1002/advs.202505009
IF:
アプリケーション: WB
交差反応性: Mouse,Human
掲載日: 2025 Apr
-
Urolithin a attenuates rheumatoid arthritis by inhibiting inflammation and pyroptosis in fibroblasts via the AMPK/ NF-κB signaling pathway
ジャーナル: International Immunopharmacology
DOI: 10.1016/j.intimp.2025.114604
IF: 4.8
アプリケーション: WB
交差反応性: Mouse
掲載日: 2025 Apr
同一ターゲット＆同一シグナル伝達経路の関連製品
Phospho-NF-kB p65 (S529) Recombinant Rabbit Monoclonal Antibody [SP07-00]
Application: WB,IHC-P,FC,IP
Reactivity: Human
Conjugate: unconjugated
NF-kB p65 Recombinant Rabbit Monoclonal Antibody [SZ10-04]
Application: WB,IF-Cell,IF-Tissue,IHC-P,IP,FC
Reactivity: Human,Mouse,Rat,Zebrafish
Conjugate: unconjugated
Phospho-NF-kB p65 (S536) Recombinant Rabbit Monoclonal Antibody [PSH10-58] - BSA and Azide free
Application: WB,IF-Cell
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
NF-kB p65 Rabbit Polyclonal Antibody
Application: WB,IHC-P,IF-Cell,FC,IP
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
NF-kB p65 Recombinant Rabbit Monoclonal Antibody [PSH0-27] - BSA and Azide free
Application: WB,IHC-P,IF-Cell,FC
Reactivity: Human,Mouse,Rat,Green monkey
Conjugate: unconjugated
Phospho-NF-kB p65 (S529) Recombinant Rabbit Monoclonal Antibody [SP07-00] - BSA and Azide free
Application: WB,IHC-P,FC,IP
Reactivity: Human
Conjugate: unconjugated
NF-kB p65 Recombinant Rabbit Monoclonal Antibody [PSH0-27]
Application: WB,IHC-P,IF-Cell,FC
Reactivity: Human,Mouse,Rat,Green monkey
Conjugate: unconjugated
NF-kB p65 Recombinant Rabbit Monoclonal Antibody [SZ10-04] - BSA and Azide free
Application: WB,IF-Cell,IF-Tissue,IHC-P,IP,FC
Reactivity: Human,Mouse,Rat,Zebrafish
Conjugate: unconjugated
