Skip to content

Phospho-AKT (T308) Recombinant Rabbit Monoclonal Antibody [PSH08-10]

見積もりを依頼する

お問い合わせ

E-mail: [email protected]

TEL: 0571-88062880

代理店・販売店を探す

Catalog# HA722951

Phospho-AKT (T308) Recombinant Rabbit Monoclonal Antibody [PSH08-10]

Application

  • WB

  • IF-Cell

  • FC

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

製品概要

製品名

Phospho-AKT (T308) Recombinant Rabbit Monoclonal Antibody [PSH08-10]

抗体のタイプ

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic phospho-peptide corresponding to residues surrounding Thr308 of Human AKT1.

製品の特異性

Phospho-Akt (Thr308) [PSH08-10] Rabbit mAb recognizes endogenous levels of Akt1 protein only when phosphorylated at Thr308. This antibody also recognizes endogenous levels of Akt2 protein when phosphorylated at Thr309 or Akt3 protein when phosphorylated at Thr305.

交差反応性（対応種属）

Human

検証済みアプリケーション

WB, IF-Cell, FC

標的分子量

Predicted band size: 56 kDa

ポジティブコントロール

Jurkat treated with 100nM Calyculin A for 40 minutes cell lysate, Jurkat cells treated with 100nM Calyculin A for 30 minutes.

コンジュゲーション

unconjugated

クローン番号

PSH08-10

Reactivity Data

Tested Tested Tested
検証済（社内検証通過）
Published Tested
文献報告済（社内未検証、文献サポートあり）
Predicted Tested
反応性予測（高い相同性に基づく）
Not recommended Tested
非推奨（社内検証未通過）
WB IHC-P FC IF-Cell
human
Tested Tested
Tested Tested
Tested Tested
Tested Tested

製品の特徴

形態

Liquid

濃度

保存方法

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

保存バッファー

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

アイソタイプ

IgG

精製方法

Protein A affinity purified.

推奨希釈倍率

  • WB

  • 1:1,000-1:2,000

  • IF-Cell

  • 1:100

  • FC

  • 1:1,000

ターゲット

機能

RAC(Rho family)-alpha serine/threonine-protein kinase is an enzyme that in humans is encoded by the AKT1 gene. This enzyme belongs to the AKT subfamily of serine/threonine kinases that contain SH2 (Src homology 2-like) protein domains. It is commonly referred to as PKB, or by both names as "Akt/PKB". The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mice lacking Akt1 display a 25% reduction in body mass, indicating that Akt1 is critical for transmitting growth-promoting signals, most likely via the IGF1 receptor. Mice lacking Akt1 are also resistant to cancer: They experience considerable delay in tumor growth initiated by the large T antigen or the Neu oncogene. A single-nucleotide polymorphism in this gene causes Proteus syndrome.

背景・参考文献

1. Huang L et al. PRMT5 activates AKT via methylation to promote tumor metastasis. Nat Commun. 2022 Jul

2. Liang XX et al. Phosphorylation of Akt at Thr308 regulates p-eNOS Ser1177 during physiological conditions. FEBS Open Bio. 2021 Jul

サブセルラー局在

Cytoplasm, Nucleus, Cell membrane.

UNIPROT #

SwissProt: P31749 Human

別名

AKT1 antibody

C AKT antibody

PKB antibody

PRKBA antibody

RAC alpha antibody

RAC alpha serine/threonine-protein kinase antibody

RAC antibody

RAC PK alpha antibody

v akt murine thymoma viral oncogene homolog 1 antibody

画像

  • Western blot analysis of Phospho-AKT (T308) on different lysates with Rabbit anti-Phospho-AKT (T308) antibody (<a href="/products/HA722951" style="font-weight: bold;text-decoration: underline;">HA722951</a>) at 1/1,000 dilution.<br /><br />Lane 1: Jurkat (Human T-lymphoblastic cells) cell lysate<br />Lane 2: Jurkat treated with 100nM Calyculin A for 40 minutes cell lysate<br />Lane 3: Jurkat treated with 100nM Calyculin A for 40 minutes cell lysate, then the membrane treated with λpp for 1 hour<br /><br />Lysates/proteins at 30 µg/Lane.<br />Exposure time: 1 minute 18 seconds; ECL: K1802<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA722951" style="font-weight: bold;text-decoration: underline;">HA722951</a>, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 56 kDa<br />Observed band size: 56 kDa

    Western blot analysis of Phospho-AKT (T308) on different lysates with Rabbit anti-Phospho-AKT (T308) antibody (HA722951) at 1/1,000 dilution.

    Lane 1: Jurkat (Human T-lymphoblastic cells) cell lysate
    Lane 2: Jurkat treated with 100nM Calyculin A for 40 minutes cell lysate
    Lane 3: Jurkat treated with 100nM Calyculin A for 40 minutes cell lysate, then the membrane treated with λpp for 1 hour

    Lysates/proteins at 30 µg/Lane.
    Exposure time: 1 minute 18 seconds; ECL: K1802

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA722951, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 56 kDa
    Observed band size: 56 kDa

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Immunocytochemistry analysis of Jurkat cells treated with or without 100nM Calyculin A for 30 minutes labeling Phospho-AKT (T308) with Rabbit anti-Phospho-AKT (T308) antibody (<a href="/products/HA722951" style="font-weight: bold;text-decoration: underline;">HA722951</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-AKT (T308) antibody (<a href="/products/HA722951" style="font-weight: bold;text-decoration: underline;">HA722951</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    ☑ Cell treatment (CT)

    Immunocytochemistry analysis of Jurkat cells treated with or without 100nM Calyculin A for 30 minutes labeling Phospho-AKT (T308) with Rabbit anti-Phospho-AKT (T308) antibody (HA722951) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-AKT (T308) antibody (HA722951) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Flow cytometric analysis of Jurkat cells treated with or without 100nM Calyculin A for 30 minutes labeling Phospho-AKT (T308).<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA722951" style="font-weight: bold;text-decoration: underline;">HA722951</a>, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    ☑ Cell treatment (CT)

    Flow cytometric analysis of Jurkat cells treated with or without 100nM Calyculin A for 30 minutes labeling Phospho-AKT (T308).

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA722951, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

文献・引用論文

ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）

同一ターゲット＆同一シグナル伝達経路の関連製品

metafield image

Phospho-AKT (S473) Recombinant Rabbit Monoclonal Antibody [PSH24-43]

Application: WB,IP

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

Phospho-AKT (S473) Recombinant Rabbit Monoclonal Antibody [SY28-05] - BSA and Azide free

Application: WB,IF-Cell,IF-Tissue,IHC-P,IHC-Fr

Reactivity: Human,Mouse,Rat,Dog

Conjugate: unconjugated

metafield image

Phospho-AKT (S473) Recombinant Rabbit Monoclonal Antibody [PSH24-43] - BSA and Azide free

Application: WB,IP

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

Phospho-AKT (S473) Recombinant Rabbit Monoclonal Antibody [PSH04-44] - BSA and Azide free

Application: WB,IHC-P,FC

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

Phospho-AKT (T308) Recombinant Rabbit Monoclonal Antibody [PSH08-10] - BSA and Azide free

Application: WB,IF-Cell,FC

Reactivity: Human

Conjugate: unconjugated

metafield image

Phospho-AKT (S473) Recombinant Rabbit Monoclonal Antibody [SY28-05]

Application: WB,IF-Cell,IF-Tissue,IHC-P,IHC-Fr

Reactivity: Human,Mouse,Rat,Dog

Conjugate: unconjugated

metafield image

Phospho-AKT (S473) Recombinant Rabbit Monoclonal Antibody [PSH04-44]

Application: WB,IHC-P,FC

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

Close (esc)

Popup

Use this popup to embed a mailing list sign up form. Alternatively use it as a simple call to action with a link to a product or a page.

Age verification

By clicking enter you are verifying that you are old enough to consume alcohol.

Enter

搜索

主菜单