CD68 Recombinant Rabbit Monoclonal Antibody [PSH06-65]
製品概要
製品名
CD68 Recombinant Rabbit Monoclonal Antibody [PSH06-65]
抗体のタイプ
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within mouse CD68 aa 1-316.
交差反応性（対応種属）
Mouse, Rat
検証済みアプリケーション
WB, IF-Cell, IHC-P, IF-Tissue, IHC-Fr
標的分子量
Predicted band size: 35 kDa
ポジティブコントロール
RAW264.7 cell lysate, M‑NFS‑60 cell lysate, J774A.1 cell lysate, Rat spleen tissue lysate, RAW264.7, mouse brain tissue, mouse intestine tissue, mouse spleen tissue, rat intestine tissue, rat spleen tissue.
コンジュゲーション
unconjugated
クローン番号
PSH06-65
Reactivity Data
検証済（社内検証通過）
文献報告済（社内未検証、文献サポートあり）
反応性予測（高い相同性に基づく）
非推奨（社内検証未通過）
| WB | IHC-P | IHC-Fr | FC | IF-Cell | IF-Tissue | |
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| mouse |
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| rat |
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製品の特徴
形態
Liquid
濃度
保存方法
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
保存バッファー
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
アイソタイプ
IgG
精製方法
Protein A affinity purified.
推奨希釈倍率
-
WB
-
1:5,000
-
IF-Cell
-
1:100
-
IHC-P
-
1:500
-
IF-Tissue
-
1:500
-
IHC-Fr
-
1:1,000
ターゲット
機能
CD68 (Cluster of Differentiation 68) is a protein highly expressed by cells in the monocyte lineage (e.g., monocytic phagocytes, osteoclasts), by circulating macrophages, and by tissue macrophages (e.g., Kupffer cells, microglia). Other names or aliases for this gene in humans and other animals include: CD68 Molecule, CD68 Antigen, GP110, Macrosialin, Scavenger Receptor Class D, Member 1, SCARD1, and LAMP4. The mouse equivalent is known as "macrosialin".
背景・参考文献
1. Choudhary M et al. CD68: Potential Contributor to Inflammation and RPE Cell Dystrophy. Adv Exp Med Biol. 2023
2. Deng R et al. Periosteal CD68(+) F4/80(+) Macrophages Are Mechanosensitive for Cortical Bone Formation by Secretion and Activation of TGF-beta1. Adv Sci (Weinh). 2022 Jan
サブセルラー局在
Endosome membrane, Lysosome membrane; Cell membrane.
別名
CD 68 antibody
CD68 antibody
CD68 antigen antibody
CD68 molecule antibody
CD68_HUMAN antibody
DKFZp686M18236 antibody
gp11 antibody
Gp110 antibody
LAMP4 antibody
Macrophage antigen CD68 (microsialin) antibody
詳細を見る画像
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☑ Relative expression (RE)
Western blot analysis of CD68 on different lysates with Rabbit anti-CD68 antibody (HA722737) at 1/5,000 dilution.
Lane 1: RAW264.7 cell lysate (20 µg/Lane)
Lane 2: M‑NFS‑60 cell lysate (20 µg/Lane)
Lane 3: J774A.1 cell lysate (20 µg/Lane)
Lane 4: NIH/3T3 cell lysate (negative) (20 µg/Lane)
Lane 5: Neuro-2a cell lysate (negative) (20 µg/Lane)
Lane 6: Rat spleen tissue lysate (no heat) (40 µg/Lane)
Notice: no heat means the lysate is not boiled.
Predicted band size: 35 kDa
Observed band size: 90-200 kDa
Exposure time: 12 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722737) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Western blot analysis of CD68 on different lysates with Rabbit anti-CD68 antibody (HA722737) at 1/5,000 dilution.
Lane 1: RAW264.7 cell lysate
Lane 2: RAW264.7 cell lysate treated with deglycosylation
Lysates/proteins at 20 µg/Lane.
Predicted band size: 35 kDa
Observed band size: 60-100 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722737) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Application: IHC-Fr
Species: Mouse
Site: Spleen
Sample: Frozen section
Antibody concentration: 1/1,000
Antigen retrieval: Not required -
Application: IHC-Fr
Species: Rat
Site: Spleen
Sample: Frozen section
Antibody concentration: 1/1,000
Antigen retrieval: Not required -
☑ Relative expression (RE)
Immunocytochemistry analysis of RAW264.7 (positive) and Neuro-2a (negative) labeling CD68 with Rabbit anti-CD68 antibody (HA722737) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD68 antibody (HA722737) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-CD68 antibody (HA722737) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722737) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse intestine tissue with Rabbit anti-CD68 antibody (HA722737) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722737) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-CD68 antibody (HA722737) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722737) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat intestine tissue with Rabbit anti-CD68 antibody (HA722737) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722737) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-CD68 antibody (HA722737) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722737) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Application: IF-tissue
Species: Mouse
Site: Spleen
Sample: Paraffin-embedded section
Antibody concentration: 1/500 -
Application: IF-tissue
Species: Rat
Site: Spleen
Sample: Paraffin-embedded section
Antibody concentration: 1/500
文献・引用論文
-
Resveratrol-Pretreated Adipose-Derived Mesenchymal Stem Cell Exosomes Regulate Macrophage Polarization via PI3K/AKT Signaling Pathway
ジャーナル: Current Molecular Pharmacology
DOI: 10.1016/j.cmp.2026.09.001
IF: 7.2
アプリケーション: IF-Tissue
交差反応性: Mouse
掲載日: 2026 Sept
-
Spatial immune atlas of breast cancer brain metastasis reveals CD163+ macrophage reprogramming associated with immune escape
ジャーナル: Journal For Immunotherapy Of Cancer
DOI: 10.1136/jitc-2025-014421
IF: 11.7
アプリケーション: mIF-Tissue
交差反応性: Mouse
掲載日: 2026 Jun
-
Synergistic Immunomodulation and Angiogenesis-Driven Microenvironment Remodeling via Bioactive Hydrogel/Nanofiber Composites for Enhanced Infectious Wound Healing
ジャーナル: Advanced Healthcare Materials
DOI: 10.1002/adhm.71354
IF: 11
アプリケーション: IF-Cell
交差反応性: Rat
掲載日: 2026 Jun
-
Biomimetic self-assembled nanosoldiers of paotianxiong polysaccharides and α-lipoic acid: Highly specific targeted therapy for mesangial proliferative glomerulonephritis
ジャーナル: Materials Today Bio
DOI: 10.1016/j.mtbio.2026.103313
IF: 11
アプリケーション: IF-Tissue,IF-Cell
交差反応性: Rat,Mouse
掲載日: 2026 Jun
-
Low-intensity pulsed ultrasound responsive phase-change nanoparticles loaded with rapamycin for targeted therapy of atherosclerotic plaques
ジャーナル: RSC Advances
DOI: 10.1039/D5RA09347C
IF: 4.6
アプリケーション: IHC
交差反応性: Mouse
掲載日: 2026 Jan
-
Self-assembling ferritin nanoplatform enables amyloid-β-targeted immunotherapy and cognitive rescue in APP/PS1 mice
ジャーナル: Brain, Behavior, And Immunity
DOI: 10.1016/j.bbi.2026.106486
IF: 7.6
アプリケーション: IF-tissue
交差反応性: Mouse
掲載日: 2026 Feb
-
Schisandra chinensis-Derived Bioactive Constituents Loaded in pH-ROS Dual-Responsive Polysaccharide Nanoparticles for Atherosclerosis Treatment
ジャーナル: Phytomedicine
DOI: 10.1016/j.phymed.2025.157536
IF: 8.3
アプリケーション: IF-tissue
交差反応性: Mouse
掲載日: 2025 Nov
-
Ultrasound-Driven Piezoelectric Heterostructures Block Early Atherosclerotic Plaques Progression
ジャーナル: Small
DOI: 10.1002/smll.202412815
IF: 12.1
アプリケーション: IF-Tissue
交差反応性: Mouse
掲載日: 2025 Mar
ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）