MTH1 Recombinant Rabbit Monoclonal Antibody [PSH02-40]

Western blot analysis of MTH1 on different lysates with Rabbit anti-MTH1 antibody (HA721817) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution.

Lane 1: HEK-293 cell lysate
Lane 2: HeLa cell lysate
Lane 3: Jurkat cell lysate
Lane 4: HepG2 cell lysate
Lane 5: K-562 cell lysate
Lane 6: A549 cell lysate
Lane 7: MCF7 cell lysate
Lane 8: SW480 cell lysate
Lane 9: THP-1 cell lysate
Lane 10: SK-OV-3 cell lysate
Lane 11: U-2 OS cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 18 kDa
Observed band size: 18 kDa

Exposure time: 5 minutes; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721817) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-MTH1 antibody (HA721817) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721817) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western blot analysis of MTH1 on different lysates with Rabbit anti-MTH1 antibody (HA721817) at 1/1,000 dilution.

Lane 1: HEK-293 cell lysate
Lane 2: NIH/3T3 cell lysate
Lane 3: C2C12 cell lysate
Lane 4: bEnd.3 cell lysate
Lane 5: RAW264.7 cell lysate
Lane 6: F9 cell lysate
Lane 7: Neuro-2a cell lysate
Lane 8: C6 cell lysate
Lane 9: L6 cell lysate
Lane 10: PC-12 cell lysate
Lane 11: Mouse thymus tissue lysate
Lane 12: Rat thymus tissue lysate
Lane 13: Mouse testis tissue lysate
Lane 14: Rat testis tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 18 kDa
Observed band size: 18 kDa

Exposure time: 5 minutes; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721817) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue with Rabbit anti-MTH1 antibody (HA721817) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721817) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human thymus tissue with Rabbit anti-MTH1 antibody (HA721817) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721817) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded rat thymus tissue with Rabbit anti-MTH1 antibody (HA721817) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721817) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

☑ Knockdown (KD)

Western blot analysis of MTH1 on different lysates with Rabbit anti-MTH1 antibody (HA721817) at 1/1,000 dilution.

Lane 1: HEK-293-si NT cell lysate
Lane 2: HEK-293-si MTH1 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 18 kDa
Observed band size: 18 kDa

Exposure time: 1 minute 34 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721817) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Flow cytometric analysis of Jurkat cells labeling MTH1.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721817,1:1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).