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TGF beta 1 Recombinant Rabbit Monoclonal Antibody [PD00-17]

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Catalog# HA721143

TGF beta 1 Recombinant Rabbit Monoclonal Antibody [PD00-17]

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

  • Monkey

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

概要

製品名

TGF beta 1 Recombinant Rabbit Monoclonal Antibody [PD00-17]

抗体の種類

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide (KLH-coupled) corresponding to Human TGF-Beta 1 C-terminal.

種属反応性

Human, Mouse, Rat, Monkey

検証された応用例

WB, IHC-P, IF-Tissue

分子量

Predicted band size: 44 kDa

陽性対照

K-562 cell lysate, Raji cell lysate, Saos-2 cell lysate, 786-0 cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, L-929 cell lysate, C6 cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, rat spleen tissue, mouse spleen tissue.

結合

unconjugated

クローン番号

PD00-17

RRID

AB_3072265

製品の特徴

形態

Liquid

濃度

保存方法

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

保存用バッファー

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

アイソタイプ

IgG

精製方法

Protein A affinity purified.

応用希釈度

  • WB

  • 1:2,000-1:5,000

  • IHC-P

  • 1:200

  • IF-Tissue

  • 1:200

ターゲット

機能

Transforming growth factor beta 1 or TGF-β1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation, and apoptosis. In humans, TGF-β1 is encoded by the TGFB1 gene. TGF-β is a multifunctional set of peptides that controls proliferation, differentiation, and other functions in many cell types. TGF-β acts synergistically with transforming growth factor-alpha (TGF-α) in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGF-β activation and signaling may result in apoptosis. Many cells synthesize TGF-β and almost all of them have specific receptors for this peptide. TGF-β1, TGF-β2, and TGF-β3 all function through the same receptor signaling systems. TGF-β1 was first identified in human platelets as a protein with a molecular mass of 25 kilodaltons with a potential role in wound healing. It was later characterized as a large protein precursor (containing 390 amino acids) that was proteolytically processed to produce a mature peptide of 112 amino acids. TGF-β1 plays an important role in controlling the immune system, and shows different activities on different types of cell, or cells at different developmental stages. Most immune cells (or leukocytes) secrete TGF-β1.

背景文献

1. Takahara T. et. al.TGFB1 mRNA expression is associated with poor prognosis and specific features of inflammation in ccRCC. Virchows Arch. 2022 Feb

2. Abdel Mouti M. et. al. TGFB1/INHBA Homodimer/Nodal-SMAD2/3 Signaling Network: A Pivotal Molecular Target in PDAC Treatment. Mol Ther. 2021 Mar

亜細胞局在

Extracellular matrix, Secreted.

UNIPROT #

SwissProt: P01137 Human

SwissProt: P04202 Mouse

SwissProt: P17246 Rat

別名

Cartilage-inducing factor antibody

CED antibody

Differentiation inhibiting factor antibody

DPD1 antibody

LAP antibody

Latency-associated peptide antibody

Prepro transforming growth factor beta 1 antibody

TGF beta 1 antibody

TGF beta antibody

TGF beta 1 protein antibody

展開

画像

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of TGF beta 1 on different lysates with Rabbit anti-TGF beta 1 antibody (<a href="/products/HA721143" style="font-weight: bold;text-decoration: underline;">HA721143</a>) at 1/5,000 dilution.<br /><br />Lane 1: K-562 cell lysate<br />Lane 2: Raji cell lysate<br />Lane 3: Saos-2 cell lysate<br />Lane 4: 786-0 cell lysate<br />Lane 5: COS-1 cell lysate<br />Lane 6: NIH/3T3 cell lysate<br />Lane 7: RAW264.7 cell lysate<br />Lane 8: L-929 cell lysate<br />Lane 9: C6 cell lysate<br />Lane 10: Mouse spleen tissue lysate<br />Lane 11: Mouse skeletal muscle tissue lysate (negative)<br />Lane 12: Rat spleen tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 44 kDa<br />Observed band size: 48/13 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721143" style="font-weight: bold;text-decoration: underline;">HA721143</a>) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of TGF beta 1 on different lysates with Rabbit anti-TGF beta 1 antibody (HA721143) at 1/5,000 dilution.

    Lane 1: K-562 cell lysate
    Lane 2: Raji cell lysate
    Lane 3: Saos-2 cell lysate
    Lane 4: 786-0 cell lysate
    Lane 5: COS-1 cell lysate
    Lane 6: NIH/3T3 cell lysate
    Lane 7: RAW264.7 cell lysate
    Lane 8: L-929 cell lysate
    Lane 9: C6 cell lysate
    Lane 10: Mouse spleen tissue lysate
    Lane 11: Mouse skeletal muscle tissue lysate (negative)
    Lane 12: Rat spleen tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 44 kDa
    Observed band size: 48/13 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721143) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-TGF beta 1 antibody (<a href="/products/HA721143" style="font-weight: bold;text-decoration: underline;">HA721143</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721143" style="font-weight: bold;text-decoration: underline;">HA721143</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-TGF beta 1 antibody (HA721143) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721143) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-TGF beta 1 antibody (<a href="/products/HA721143" style="font-weight: bold;text-decoration: underline;">HA721143</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721143" style="font-weight: bold;text-decoration: underline;">HA721143</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-TGF beta 1 antibody (HA721143) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721143) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ご注意ください: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

論文での実績

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