Biotin Conjugated TGF beta 1 Recombinant Rabbit Monoclonal Antibody [PSH15-22] - Detector

Sandwich ELISA analysis of Human TGF beta 1 matched pair antibodies

Capture: HA723725, TGF beta 1 Rabbit mAb [PSH15-21]
Detector: HA723726, TGF beta 1 Rabbit mAb [PSH15-22]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723725) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human TGF beta 1 protein starting from 20000 pg/ml to 0 pg/ml and detect antibody (HA723726, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native TGF beta 1 in mouse samples.

Capture: HA723725, TGF beta 1 Rabbit mAb [PSH15-21]
Detector: HA723726, TGF beta 1 Rabbit mAb [PSH15-22]

Interpolated concentration of native TGF beta 1 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean TGF beta 1 concentration was determined to be 103 ng/mL in mouse serum.

Interpolated concentrations of spiked TGF beta 1 in cell culture media samples.

Capture: HA723725, TGF beta 1 Rabbit mAb [PSH15-21]
Detector: HA723726, TGF beta 1 Rabbit mAb [PSH15-22]

The concentrations of TGF beta 1 were measured in duplicates, interpolated from the TGF beta 1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).