CDX2 Recombinant Rabbit Monoclonal Antibody [SY09-02]
Catalog# ET1605-4
CDX2 Recombinant Rabbit Monoclonal Antibody [SY09-02]
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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FC
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CUT&Tag-seq
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Human
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Mouse
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Rat
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unconjugated
製品概要
製品名
CDX2 Recombinant Rabbit Monoclonal Antibody [SY09-02]
抗体のタイプ
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within human CDX2 aa 20-60.
交差反応性（対応種属）
Human, Mouse, Rat
検証済みアプリケーション
WB, IF-Cell, IF-Tissue, IHC-P, FC, CUT&Tag-seq
標的分子量
Predicted band size: 34 kDa
ポジティブコントロール
Caco-2 cell lysate, LoVo cell lysate, AGS cell lysate, LOVO, AGS, human appendix tissue, Human stomach cancer tissue, human colon cancer tissue, human colon tissue, mouse colon tissue, rat colon tissue, Hela.
コンジュゲーション
unconjugated
クローン番号
SY09-02
RRID
製品の特徴
形態
Liquid
濃度
保存方法
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
保存バッファー
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
アイソタイプ
IgG
精製方法
Protein A affinity purified.
推奨希釈倍率
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WB
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1:1,000-1:2,000
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IF-Cell
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1:1,000-1:2,000
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IF-Tissue
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1:500-1:2,000
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IHC-P
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1:5,000-1:20,000
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FC
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1:1,000
ターゲット
機能
In common with the two other Cdx genes, CDX2 regulates several essential processes in the development and function of the lower gastrointestinal tract (from the duodenum to the anus) in vertebrates. In vertebrate embryonic development, CDX2 becomes active in endodermal cells that are posterior to the developing stomach.These cells eventually form the intestinal epithelium. The activity of CDX2 at this stage is essential for the correct formation of the intestine and the anus.CDX2 is also required for the development of the placenta.
背景・参考文献
1. Yang Y et al. Heightened potency of human pluripotent stem cell lines created by transient BMP4 exposure. Proc Natl Acad Sci U S A 112:E2337-46 (2015).
2. Ohinata Y & Tsukiyama T Establishment of trophoblast stem cells under defined culture conditions in mice. PLoS One 9:e107308 (2014).
配列相同性
Belongs to the Caudal homeobox family.
翻訳後修飾（PTM）
Phosphorylation of Ser-60 mediates the transactivation capacity.
サブセルラー局在
Nucleus.
別名
Caudal type homeo box 2 antibody
Caudal type homeo box transcription factor 2 antibody
Caudal type homeobox 2 antibody
Caudal type homeobox protein 2 antibody
Caudal type homeobox transcription factor 2 antibody
Caudal-type homeobox protein 2 antibody
CDX 2 antibody
CDX 3 antibody
CDX-3 antibody
Cdx2 antibody
詳細を見る画像
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Western blot analysis of CDX2 on different lysates with Rabbit anti-CDX2 antibody (ET1605-4) at 1/1,000 dilution.
Lane 1: Caco-2 cell lysate
Lane 2: LoVo cell lysate
Lane 3: AGS cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-4) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of CDX2 on different lysates with Rabbit anti-CDX2 antibody (ET1605-4) at 1/1,000 dilution.
Lane 1: MCF7-si NT cell lysate
Lane 2: MCF7-si CDX2 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-4) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Application: Immunocytochemistry (IF-cell)
Species: Human
Sample: Caco-2 (Human colorectal adenocarcinoma cell)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: ET1605-4, 1/2,000, overnight at 4℃.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue). -
Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-CDX2 antibody (ET1605-4) at 1/5,000 dilution.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. The section was incubated with ET1605-4 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-CDX2 antibody (ET1605-4) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-4) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-CDX2 antibody (ET1605-4) at 1/20,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-4) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-CDX2 antibody (ET1605-4) at 1/20,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-4) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-CDX2 antibody (ET1605-4) at 1/20,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-4) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-CDX2 antibody (ET1605-4) at 1/20,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-4) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded human thymus tissue (negative) with Rabbit anti-CDX2 antibody (ET1605-4) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-4) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded rat thymus tissue (negative) with Rabbit anti-CDX2 antibody (ET1605-4) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-4) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Application: Flow Cytometry (Intra)
Species: Human
Sample: Caco-2 (Human Caucasian colon adenocarcinoma)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: ET1605-4(1 μg/mL) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.
ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）
文献・引用論文
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High-Glucose-Induced Metabolic and Epithelial Stress in Grass Carp Intestinal Epithelial Cells Associated with Methylation-Related Transcriptional Responses
ジャーナル: International Journal Of Molecular Sciences
DOI: 10.3390/ijms27135732
IF: 5.6
アプリケーション: WB
交差反応性: Grass carp
掲載日: 2026 Jun
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SPP1 Drives Colorectal Cancer Liver Metastasis and Immunotherapy Resistance by Stimulating CXCL12 Production in Cancer-Associated Fibroblasts
ジャーナル: Cancer Research
DOI: 10.1158/0008-5472.CAN-24-4916
IF: 16.6
アプリケーション: IHC
交差反応性: Human
掲載日: 2026 Jan
-
Amelioration of colorectal cancer-associated fibroblasts in immunosuppressive microenvironment by ferroptosis-based nanotherapy
ジャーナル: Nature Communications
DOI: 10.1038/s41467-026-69462-5
IF: 15.7
アプリケーション: IHC
交差反応性: Human
掲載日: 2026 Feb
-
Tumor suppressor SLC9A2 inhibits colorectal cancer metastasis and reverses immunotherapy resistance by suppressing angiogenesis
ジャーナル: Journal Of Experimental & Clinical Cancer Research
DOI: 10.1186/s13046-025-03422-7
IF: 12.8
アプリケーション: IHC
交差反応性: Mouse,Human
掲載日: 2025 Jun
-
Microfluidic droplet encapsulation‐guided organoid growth promotes parental tumor phenotype recapitulation
ジャーナル: International Journal Of Cancer
DOI:
IF: 6.4
アプリケーション: IHC-P
交差反応性: Human
掲載日: 2023 Aug