Albumin Rabbit Polyclonal Antibody

Western blot analysis of Albumin on different lysates with Rabbit anti-Albumin antibody (0806-9) at 1/20,000 dilution.

Lane 1: Hep G2 (Human hepatocellular carcinoma cell) cell lysate (15 µg/Lane)
Lane 2: Mouse liver tissue lysate (30 µg/Lane)
Lane 3: Rat brain liver lysate(30 µg/Lane)

Exposure time: 180 seconds ; ECL: K1802

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: 0806-9, 1/20,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 67 kDa
Observed band size: 67 kDa

ICC staining Albumin in D3 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Albumin antibody (0806-9) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (0806-9) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow cytometric analysis of HepG2 cells labeling Albumin.

Cells were fixed and permeabilized. Then stained with the primary antibody (0806-9, 1/50) (red) . After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).