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NF-H Recombinant Rabbit Monoclonal Antibody [JF99-07] - BSA and Azide free

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Catalog# HA750357

NF-H Recombinant Rabbit Monoclonal Antibody [JF99-07] - BSA and Azide free

Application

  • WB

  • IHC-P

  • IHC-Fr

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Predicted reactivity

  • Cynomolgus monkey

  • Pig

予測される交差反応性
Conjugation

  • unconjugated

製品概要

製品名

NF-H Recombinant Rabbit Monoclonal Antibody [JF99-07] - BSA and Azide free

抗体のタイプ

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within human NEFH aa 690-740.

交差反応性（対応種属）

Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)

予測される交差反応性

検証済みアプリケーション

WB, IHC-P, IHC-Fr, IF-Tissue

標的分子量

Predicted band size: 112 kDa

ポジティブコントロール

Mouse brain tissue lysate, Rat brain tissue lysate, Rat hippocampus tissue lysate, human cerebellum tissue, mouse cerebellum tissue, rat cerebellum tissue.

コンジュゲーション

unconjugated

クローン番号

JF99-07

製品の特徴

形態

Liquid

濃度

保存方法

Store at 2-8℃. Avoid freeze.

保存バッファー

1*PBS (pH7.4).

アイソタイプ

IgG

精製方法

Protein A affinity purified.

推奨希釈倍率

ターゲット

機能

Neurofilament-H (NF-H), for neurofilament heavy polypeptide, a member of the intermediate filament family, is a major component of neuronal cytoskeletons. Neurofilaments are dynamic structures; they contain phosphorylation sites for a large number of protein kinases, including protein kinase A, protein kinase C, cyclin-dependent kinase 5, extracellular signal regulated kinase, glycogen synthase kinase-3, and stress-activated protein kinase gamma. In addition to their role in the control of axon caliber, neurofilaments may affect other cytoskeletal elements, such as microtubules and Actin filaments. Changes in neurofilament phosphorylation or metabolism are frequently observed in neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), Parkinson's disease and Alzheimer's disease.

背景・参考文献

1. Wang J et al. Unveiling the NEFH+ malignant cell subtype: Insights from single-cell RNA sequencing in prostate cancer progression and tumor microenvironment interactions. Front Immunol. 2024 Dec

2. Koudonas A et al. Methylation of PCDH17 and NEFH as prognostic biomarker for nonmetastatic RCC: A cohort study. Medicine (Baltimore). 2022 Jul

配列相同性

Belongs to the intermediate filament family.

翻訳後修飾（PTM）

There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.; Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function.; Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.

サブセルラー局在

Cytoplasm, cytoskeleton, Cell projection, axon.

UNIPROT #

SwissProt: P12036 Human

SwissProt: P19246 Mouse

SwissProt: P16884 Rat

別名

200 kDa neurofilament protein antibody

CMT2CC antibody

NF200 antibody

Nefh antibody

Neurofilament heavy polypeptide 200kDa antibody

Neurofilament heavy polypeptide antibody

Neurofilament triplet H protein antibody

NF H antibody

NF-H antibody

NFH antibody

詳細を見る

画像

  • This data was developed using <a href="/products/ET1702-72" style="font-weight: bold;text-decoration: underline;">ET1702-72</a>, the same antibody clone in a different buffer formulation.<br />Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebellum <br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:2,000<br /><br />Antigen retrieval: Not required

    This data was developed using ET1702-72, the same antibody clone in a different buffer formulation.
    Application: IHC-Fr

    Species: Mouse

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1:2,000

    Antigen retrieval: Not required

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />This data was developed using <a href="/products/ET1702-72" style="font-weight: bold;text-decoration: underline;">ET1702-72</a>, the same antibody clone in a different buffer formulation.<br />Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Liver (Negative)<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:500<br /><br />Antigen retrieval: Not required

    ☑ Relative expression (RE)

    This data was developed using ET1702-72, the same antibody clone in a different buffer formulation.
    Application: IHC-Fr

    Species: Mouse

    Site: Liver (Negative)

    Sample: Frozen section

    Antibody concentration: 1:500

    Antigen retrieval: Not required

  • This data was developed using <a href="/products/ET1702-72" style="font-weight: bold;text-decoration: underline;">ET1702-72</a>, the same antibody clone in a different buffer formulation.<br />Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: Cerebellum <br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:500<br /><br />Antigen retrieval: Not required

    This data was developed using ET1702-72, the same antibody clone in a different buffer formulation.
    Application: IHC-Fr

    Species: Rat

    Site: Cerebellum

    Sample: Frozen section

    Antibody concentration: 1:500

    Antigen retrieval: Not required

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />This data was developed using <a href="/products/ET1702-72" style="font-weight: bold;text-decoration: underline;">ET1702-72</a>, the same antibody clone in a different buffer formulation.<br />Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: Liver (Negative)<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1:500<br /><br />Antigen retrieval: Not required

    ☑ Relative expression (RE)

    This data was developed using ET1702-72, the same antibody clone in a different buffer formulation.
    Application: IHC-Fr

    Species: Rat

    Site: Liver (Negative)

    Sample: Frozen section

    Antibody concentration: 1:500

    Antigen retrieval: Not required

  • This data was developed using <a href="/products/ET1702-72" style="font-weight: bold;text-decoration: underline;">ET1702-72</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of NF-H on different lysates with Rabbit anti-NF-H antibody (<a href="/products/ET1702-72" style="font-weight: bold;text-decoration: underline;">ET1702-72</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate (20 µg/Lane)<br />Lane 2: Rat brain tissue lysate (20 µg/Lane)<br />Lane 3: Rat hippocampus tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 112 kDa<br />Observed band size: 250 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1702-72" style="font-weight: bold;text-decoration: underline;">ET1702-72</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    This data was developed using ET1702-72, the same antibody clone in a different buffer formulation.
    Western blot analysis of NF-H on different lysates with Rabbit anti-NF-H antibody (ET1702-72) at 1/2,000 dilution.

    Lane 1: Mouse brain tissue lysate (20 µg/Lane)
    Lane 2: Rat brain tissue lysate (20 µg/Lane)
    Lane 3: Rat hippocampus tissue lysate (20 µg/Lane)

    Predicted band size: 112 kDa
    Observed band size: 250 kDa

    Exposure time: 3 minutes; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-72) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • This data was developed using <a href="/products/ET1702-72" style="font-weight: bold;text-decoration: underline;">ET1702-72</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-NF-H antibody (<a href="/products/ET1702-72" style="font-weight: bold;text-decoration: underline;">ET1702-72</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1702-72" style="font-weight: bold;text-decoration: underline;">ET1702-72</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using ET1702-72, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-NF-H antibody (ET1702-72) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-72) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/ET1702-72" style="font-weight: bold;text-decoration: underline;">ET1702-72</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-NF-H antibody (<a href="/products/ET1702-72" style="font-weight: bold;text-decoration: underline;">ET1702-72</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1702-72" style="font-weight: bold;text-decoration: underline;">ET1702-72</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using ET1702-72, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-NF-H antibody (ET1702-72) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-72) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/ET1702-72" style="font-weight: bold;text-decoration: underline;">ET1702-72</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-NF-H antibody (<a href="/products/ET1702-72" style="font-weight: bold;text-decoration: underline;">ET1702-72</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1702-72" style="font-weight: bold;text-decoration: underline;">ET1702-72</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using ET1702-72, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-NF-H antibody (ET1702-72) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-72) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/ET1702-72" style="font-weight: bold;text-decoration: underline;">ET1702-72</a>, the same antibody clone in a different buffer formulation.<br />Application: IF-tissue<br /><br />Species: Mouse<br /><br />Site: Cerebellum<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1:500

    This data was developed using ET1702-72, the same antibody clone in a different buffer formulation.
    Application: IF-tissue

    Species: Mouse

    Site: Cerebellum

    Sample: Paraffin-embedded section

    Antibody concentration: 1:500

ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）

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