NF-H Recombinant Antibody [JF99-07] - Guinea pig IgG2 (Chimeric)
概要
製品名
NF-H Recombinant Antibody [JF99-07] - Guinea pig IgG2 (Chimeric)
抗体の種類
Recombinant Chimeric Antibody
免疫原
Synthetic peptide within human NEFH aa 690-740.
種属反応性
Human, Mouse, Rat
検証された応用例
IHC-P
分子量
Predicted band size: 112 kDa
陽性対照
Human cerebellum tissue, mouse cerebellum tissue, rat cerebellum tissue.
結合
unconjugated
クローン番号
JF99-07
製品の特徴
形態
Liquid
保存方法
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
保存用バッファー
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
精製方法
Protein A affinity purified.
応用希釈度
-
IHC-P
-
1:2,000
ターゲット
機能
Neurofilament-H (NF-H), for neurofilament heavy polypeptide, a member of the intermediate filament family, is a major component of neuronal cytoskeletons. Neurofilaments are dynamic structures; they contain phosphorylation sites for a large number of protein kinases, including protein kinase A, protein kinase C, cyclin-dependent kinase 5, extracellular signal regulated kinase, glycogen synthase kinase-3, and stress-activated protein kinase gamma. In addition to their role in the control of axon caliber, neurofilaments may affect other cytoskeletal elements, such as microtubules and Actin filaments. Changes in neurofilament phosphorylation or metabolism are frequently observed in neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), Parkinson's disease and Alzheimer's disease.
背景文献
1. Wang J et al. Unveiling the NEFH+ malignant cell subtype: Insights from single-cell RNA sequencing in prostate cancer progression and tumor microenvironment interactions. Front Immunol. 2024 Dec
2. Koudonas A et al. Methylation of PCDH17 and NEFH as prognostic biomarker for nonmetastatic RCC: A cohort study. Medicine (Baltimore). 2022 Jul
亜細胞局在
Cytoplasm, cytoskeleton, Cell projection, axon.
別名
200 kDa neurofilament protein antibody
CMT2CC antibody
Nefh antibody
Neurofilament heavy polypeptide 200kDa antibody
Neurofilament heavy polypeptide antibody
Neurofilament triplet H protein antibody
NF H antibody
NF-H antibody
NFH antibody
NFH_HUMAN antibody
画像
-
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Guinea pig anti-NF-H antibody (HA601581) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601581) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Guinea pig anti-NF-H antibody (HA601581) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601581) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Guinea pig anti-NF-H antibody (HA601581) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601581) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ご注意ください: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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