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Lamin B1 Recombinant Rabbit Monoclonal Antibody [SI17-07]

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Catalog# ET1606-27

Lamin B1 Recombinant Rabbit Monoclonal Antibody [SI17-07]

Application

  • WB

  • IF-Tissue

  • IHC-P

  • CUT&Tag-seq

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

概要

製品名

Lamin B1 Recombinant Rabbit Monoclonal Antibody [SI17-07]

抗体の種類

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within Human Lamin B1 aa 511-560 / 586.

種属反応性

Human, Mouse, Rat

検証された応用例

WB, IF-Tissue, IHC-P, CUT&Tag-seq

分子量

Predicted band size: 66 kDa

陽性対照

HeLa cell lysate, HEK-293 cell lysate, THP-1 cell lysate, SH-SY5Y cell lysate, C2C12 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, PC-12 cell lysate, human liver tissue, human breast carcinoma tissue, human colon carcinoma tissue, human lymph nodes tissue, mouse large intestine tissue, rat liver tissue.

結合

unconjugated

クローン番号

SI17-07

RRID

AB_3069729

製品の特徴

形態

Liquid

濃度

保存方法

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

保存用バッファー

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

アイソタイプ

IgG

精製方法

Protein A affinity purified.

応用希釈度

  • WB

  • 1:50,000

  • IF-Tissue

  • 1:500-1:1,000

  • IHC-P

  • 1:500-1:1,000

ターゲット

機能

A unique family of cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, termed Ced-3/ICE, functions as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Nuclear lamins are critical to maintaining the integrity of the nuclear envelope and cellular morphology as components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. Nuclear Lamin B is fragmented as a consequence of apoptosis by an unidentified member of the ICE family.

背景文献

1. Cruz OH et al. Multinucleation and Polykaryon Formation is Promoted by the EhPC4 Transcription Factor in Entamoeba histolytica. Sci Rep 6:19611 (2016).

2. Lund K et al. Slug-dependent upregulation of L1CAM is responsible for the increased invasion potential of pancreatic cancer cells following long-term 5-FU treatment. PLoS One 10:e0123684 (2015).

配列相同性

Belongs to the intermediate filament family.

翻訳後修飾

B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.

亜細胞局在

Nucleus inner membrane.

UNIPROT #

SwissProt: P20700 Human

SwissProt: P14733 Mouse

SwissProt: P70615 Rat

別名

ADLD antibody

lamin B1 antibody

Lamin-B1 antibody

LMN antibody

LMN2 antibody

LMNB antibody

Lmnb1 antibody

LMNB1_HUMAN antibody

MGC111419 antibody

OTTHUMP00000159218 antibody

画像

  • Western blot analysis of Lamin B1 on different lysates with Rabbit anti-Lamin B1 antibody (<a href="/products/ET1606-27" style="font-weight: bold;text-decoration: underline;">ET1606-27</a>) at 1/50,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: HEK-293 cell lysate<br />Lane 3: THP-1 cell lysate<br />Lane 4: SH-SY5Y cell lysate<br />Lane 5: C2C12 cell lysate<br />Lane 6: NIH/3T3 cell lysate<br />Lane 7: C6 cell lysate<br />Lane 8: PC-12 cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 66 kDa<br />Observed band size: 66 kDa<br /><br />Exposure time: 21 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1606-27" style="font-weight: bold;text-decoration: underline;">ET1606-27</a>) at 1/50,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Lamin B1 on different lysates with Rabbit anti-Lamin B1 antibody (ET1606-27) at 1/50,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: HEK-293 cell lysate
    Lane 3: THP-1 cell lysate
    Lane 4: SH-SY5Y cell lysate
    Lane 5: C2C12 cell lysate
    Lane 6: NIH/3T3 cell lysate
    Lane 7: C6 cell lysate
    Lane 8: PC-12 cell lysate

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 66 kDa
    Observed band size: 66 kDa

    Exposure time: 21 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-27) at 1/50,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockout (KO)</span><br /><br />All lanes: Western blot analysis of Lamin B1 with anti-Lamin B1 antibody [SI17-06] (<a href="/products/ET1606-27" style="font-weight: bold;text-decoration: underline;">ET1606-27</a>) at 1:1,000 dilution.<br />Lane 1/2: Wild-type Hela whole cell lysate (20 µg).<br />Lane 3/4: Lamin B1 knockout Hela whole cell lysate (20 µg).<br /><br /><a href="/products/ET1606-27" style="font-weight: bold;text-decoration: underline;">ET1606-27</a> was shown to specifically react with Lamin B1 in wild-type Hela cells. No band was observed when Lamin B1 knockout samples were tested. Wild-type and Lamin B1 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1606-27" style="font-weight: bold;text-decoration: underline;">ET1606-27</a>, 1/1,000) and Loading control antibody(Rabbit anti-Vinculin, <a href="/products/ET1705-94" style="font-weight: bold;text-decoration: underline;">ET1705-94</a>, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    ☑ Knockout (KO)

    All lanes: Western blot analysis of Lamin B1 with anti-Lamin B1 antibody [SI17-06] (ET1606-27) at 1:1,000 dilution.
    Lane 1/2: Wild-type Hela whole cell lysate (20 µg).
    Lane 3/4: Lamin B1 knockout Hela whole cell lysate (20 µg).

    ET1606-27 was shown to specifically react with Lamin B1 in wild-type Hela cells. No band was observed when Lamin B1 knockout samples were tested. Wild-type and Lamin B1 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1606-27, 1/1,000) and Loading control antibody(Rabbit anti-Vinculin, ET1705-94, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Lamin B1 antibody (<a href="/products/ET1606-27" style="font-weight: bold;text-decoration: underline;">ET1606-27</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1606-27" style="font-weight: bold;text-decoration: underline;">ET1606-27</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Lamin B1 antibody (ET1606-27) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-27) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Lamin B1 antibody (<a href="/products/ET1606-27" style="font-weight: bold;text-decoration: underline;">ET1606-27</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1606-27" style="font-weight: bold;text-decoration: underline;">ET1606-27</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Lamin B1 antibody (ET1606-27) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-27) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Lamin B1 antibody (<a href="/products/ET1606-27" style="font-weight: bold;text-decoration: underline;">ET1606-27</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1606-27" style="font-weight: bold;text-decoration: underline;">ET1606-27</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Lamin B1 antibody (ET1606-27) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-27) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-Lamin B1 antibody (<a href="/products/ET1606-27" style="font-weight: bold;text-decoration: underline;">ET1606-27</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1606-27" style="font-weight: bold;text-decoration: underline;">ET1606-27</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-Lamin B1 antibody (ET1606-27) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-27) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-Lamin B1 antibody (<a href="/products/ET1606-27" style="font-weight: bold;text-decoration: underline;">ET1606-27</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1606-27" style="font-weight: bold;text-decoration: underline;">ET1606-27</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-Lamin B1 antibody (ET1606-27) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-27) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Lamin B1 antibody (<a href="/products/ET1606-27" style="font-weight: bold;text-decoration: underline;">ET1606-27</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1606-27" style="font-weight: bold;text-decoration: underline;">ET1606-27</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Lamin B1 antibody (ET1606-27) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-27) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: IF-Tissue<br /><br />Species: Human<br /><br />Site: breast carcinoma<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/1,000

    Application: IF-Tissue

    Species: Human

    Site: breast carcinoma

    Sample: Paraffin-embedded section

    Antibody concentration: 1/1,000

ご注意ください: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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