CD11c Recombinant Rabbit Monoclonal Antibody
Catalog# IRS001
CD11c Recombinant Rabbit Monoclonal Antibody
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mIHC
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Human
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unconjugated
製品概要
製品名
CD11c Recombinant Rabbit Monoclonal Antibody
抗体のタイプ
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human CD11c aa 1,114-1,163 / 1,163 (Cytoplasmic).
交差反応性（対応種属）
Human
検証済みアプリケーション
mIHC
ポジティブコントロール
Human cholangiocarcinoma tissue, human tonsil tissue, human colon tissue, human kidney tissue.
コンジュゲーション
unconjugated
製品の特徴
形態
Liquid
濃度
保存方法
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
保存バッファー
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
アイソタイプ
IgG
精製方法
Protein A affinity purified.
推奨希釈倍率
-
mIHC
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1:100. This antibody has been validated for multiplex staining with LUMINIRIS mTSA, supporting both multiplex and super-multiplex staining.
ターゲット
機能
Integrin αX (CD11C, leukocyte surface antigen p150,95, CR4, Axb2) is a type 1 transmembrane protein that traditionally combines with β2 chain to form a leukocyte-specific integrin known as inactivated-C3b (iC3b) receptor 4 (CR4). Integrin αX/β2 shares similar properties of the αM/β2 integrin in mediating adherence of neutrophils and monocytes to stimulated endothelial cells, and in phagocytosis of complement coated particles. Abnormal expression of Integrin αX is characteristic of hairy cell leukemia (HCL) and is dependent upon activation of proto-oncogenes Ras and JunD. Proteins and DNA elements that influence transcription of Integrin αX include Sp1 and Sp1-like factors, AP-1 family, C/EBP, Oct-2 and PU.1. Integrin αX is present on monocyte derivative dendritic cells (DCs), macrophages and NK cells. Upon activation, DCs present in skin (Langerhans cells), lining of nose, lung, stomach, intestine and blood can migrate to lymphoid tissues and interact with T and B cells to initiate and shape the immune response.
背景・参考文献
1. Morandi F et al. IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation. J Immunol 192:2634-42 (2014).
2. Svensson MN et al. Fms-like tyrosine kinase 3 ligand controls formation of regulatory T cells in autoimmune arthritis. PLoS One 8:e54884 (2013).
配列相同性
Belongs to the integrin alpha chain family.
組織特異性
Predominantly expressed in monocytes and granulocytes.
サブセルラー局在
Membrane.
UNIPROT #
別名
95 alpha chain antibody
95 antibody
CD 11c antibody
CD11 antigen-like family member C antibody
CD11c antibody
CD11c antigen antibody
Complement component 3 receptor 4 subunit antibody
CR4 antibody
Integrin alpha X antibody
Integrin alpha X chain antibody
詳細を見る画像
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mIHC analysis of human cholangiocarcinoma tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD11c (IRS001), CD20 (IRS003), CD8 and panCK (IRS010) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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mIHC analysis of human cholangiocarcinoma tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD11c (IRS001), CD68 (IRS004), CD20 (IRS003), CD38 (IRS006), CD8 and panCK (IRS010) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD8, CD4 (IRS005), FOXP3, CD68 (IRS004), CD38 (IRS006), CD11c (IRS001), PD-L1, CD163 (IRS002) and CD20 (IRS003) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Ki67 (IRS036), CD11c (IRS001), PD-1 and CD8 (IRS007) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD38 (IRS006), CD57 (IRS027), CD11c (IRS001), Tryptase (IRS041) and CD45 (IRS025) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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mIHC analysis of human tonsil tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD11c antibody (IRS001) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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mIHC analysis of human colon tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD11c antibody (IRS001) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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mIHC analysis of human craniopharyngioma tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD11c antibody (IRS001) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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mIHC analysis of human kidney tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD11c antibody (IRS001) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）
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