Human/Monkey/Canine Osteopontin（OPN） enzyme-linked immunoassay kit
Catalog# EH0057
Human/Monkey/Canine Osteopontin（OPN） enzyme-linked immunoassay kit
-
ELISA
-
Human
-
0.31~20 ng/ml
-
unconjugated
製品概要
製品名
Human/Monkey/Canine Osteopontin（OPN） enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
0.04 ng/ml (50 μl)；0.12 ng/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0246 | 0.0244 | 0.0245 | |
| 0.31 | 0.0543 | 0.0502 | 0.0523 | 0.0278 |
| 0.63 | 0.0937 | 0.0866 | 0.0902 | 0.0657 |
| 1.25 | 0.2017 | 0.1903 | 0.1960 | 0.1715 |
| 2.50 | 0.5132 | 0.5027 | 0.5080 | 0.4835 |
| 5.00 | 1.0570 | 0.9935 | 1.0253 | 1.0008 |
| 10.00 | 1.9270 | 1.8780 | 1.9025 | 1.8780 |
| 20.00 | 3.0580 | 2.9620 | 3.0100 | 2.9855 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 0.42 | 2.09 | 6.22 | 0.51 | 2.10 | 6.03 |
| Standard Deviation | 0.02 | 0.09 | 0.48 | 0.02 | 0.10 | 0.37 |
| Coefficient of Variation (%) | 4.9 | 4.5 | 7.7 | 4.3 | 4.7 | 6.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested Twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human OPN into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 83% to 117% with an overall mean recovery of 108%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Human Serum | 30 | 1.10-6.74 | 100 | 3.97 |
| Monkey Serum | 2 | 6.28-6.57 | 100 | 6.43 |
| Canine Serum | 5 | 1.22-1.43 | 100 | 1.33 |
Serum/Plasma – Thirty samples from apparently healthy volunteers, two samples from healthy monkeys and five samples from healthy canines were evaluated for the presence of human OPN in this assay. No medical histories were available for the donors.
n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
ターゲット
UNIPROT #
別名
BNSP
Bone sialoprotein 1
BSP I
BSPI
Early T lymphocyte activation 1
ETA 1
ETA1
MGC110940
Nephropontin
OPN
展開