Human/Monkey/Canine CXCL10/IP-10 enzyme-linked immunoassay kit
Catalog# EH0026
Human/Monkey/Canine CXCL10/IP-10 enzyme-linked immunoassay kit
-
ELISA
-
Human
-
2.74~2000 pg/ml
製品概要
製品名
Human/Monkey/Canine CXCL10/IP-10 enzyme-linked immunoassay kit
製品タイプ
Elisa Kit
検出感度
0.16 pg/ml (50 μl)；0.68 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0150 | 0.0145 | 0.0148 | |
| 2.74 | 0.0382 | 0.0344 | 0.0363 | 0.0216 |
| 8.23 | 0.0861 | 0.0827 | 0.0844 | 0.0697 |
| 24.69 | 0.2315 | 0.2262 | 0.2289 | 0.2141 |
| 74.07 | 0.6512 | 0.6404 | 0.6458 | 0.6311 |
| 222.22 | 1.8430 | 1.6300 | 1.7365 | 1.7218 |
| 666.67 | 3.1870 | 3.1500 | 3.1685 | 3.1538 |
| 2000.00 | 3.9030 | 3.8160 | 3.8595 | 3.8448 |
精度
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 52.3 | 262.9 | 708.7 | 42.8 | 226.4 | 619.0 |
| Standard Deviation | 3.2 | 12.6 | 39.7 | 1.4 | 9.9 | 10.4 |
| Coefficient of Variation (%) | 6.1 | 4.8 | 5.6 | 3.3 | 4.4 | 1.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested Twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
スパイク回収
The spike recovery was evaluated by spiking 3 levels of human CXCL10 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 117% with an overall mean recovery of 100%.
サンプル値
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Human Serum | 30 | 8.36-99.65 | 100.0 | 25.02 |
| Monkey Serum | 2 | 101.58-109.84 | 100.0 | 105.71 |
| Canine Serum | 5 | 4.04-12.60 | 100.0 | 7.62 |
Serum/Plasma – Thirty samples from apparently healthy volunteers, two samples from healthy monkeys and five samples from healthy canines were evaluated for the presence of human CXCL10 in this assay. No medical histories were available for the donors.
n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
実験タイプ
種属反応性
検出方法
所要時間
標準曲線梯度
インキュベーション回数
2
Detectable Sample
サンプル添加量
製品の特徴
保存条件
2-8 ℃で保存
文献におけるアプリケーション
| ELISA | 下記の<%= count %>篇の文献を見る |
文献におけるアプリケーション
| Human | 下記の<%= count %>篇の文献を見る |
ターゲット
UNIPROT #
別名
Interferon gamma induced factor MOB1, mouse, homolog of
Interferon gamma induced protein 10
10 kDa interferon gamma induced protein
10 kDa interferon gamma-induced protein
C X C motif chemokine 10
C7
Chemokine (C X C motif) ligand 10
Chemokine CXC motif ligand 10
Crg 2
CRG2
展開機能
文献・引用論文
-
The cGAS-STING pathway activates transcription factor TFEB to stimulate lysosome biogenesis and pathogen clearance
ジャーナル: Immunity
DOI:
IF: 25.5
アプリケーション: ELISA
交差反応性: Human
掲載日: 2024 Dec