Albumin Recombinant Rabbit Monoclonal Antibody [PSH24-72] - BSA and Azide free

☑ Relative expression (RE)

This data was developed using HA724468, the same antibody clone in a different buffer formulation.
Western blot analysis of Albumin on different lysates with Rabbit anti-Albumin antibody (HA724468) at 1/100,000 dilution.

Lane 1: Hep G2 (Human liver cancer cell) cell lysate
Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate

Lysates/proteins at 15 µg/Lane.
Exposure time: 10 seconds; ECL: K1801

Negative expression of Albumin protein in HeLa cell is consistent with the predicted expression pattern.

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724468, 1/100,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 69 kDa
Observed band size: 69 kDa

This data was developed using HA724468, the same antibody clone in a different buffer formulation.
Western blot analysis of Albumin on different lysates with Rabbit anti-Albumin antibody (HA724468) at 1/100,000 dilution.

Lane 1: Mouse liver tissue lysate
Lane 2: Rat liver tissue lysate

Lysates/proteins at 30 µg/Lane.
Exposure time: 10 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724468, 1/100,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 69 kDa
Observed band size: 69 kDa

☑ Relative expression (RE)

This data was developed using HA724468, the same antibody clone in a different buffer formulation.
Application: Immunocytochemistry (IF-cell)

Species: Human
Sample: Hep G2 (Human liver cancer cell)/HeLa (Human cervical adenocarcinoma cell)

Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.

Primary antibody: HA724468, 1/100, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).

Negative expression of Albumin protein in HeLa cell is consistent with the predicted expression pattern.

This data was developed using HA724468, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)

Species: Rat
Tissue: Kidney
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724468, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.

This data was developed using HA724468, the same antibody clone in a different buffer formulation.
Application: Immunohistochemistry (IHC-P)

Species: Rat
Tissue: Liver
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724468, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.

This data was developed using HA724468, the same antibody clone in a different buffer formulation.
Application: Immunofluorescence (IF-tissue)

Species: Mouse
Tissue: Liver
Sample: Paraffin-embedded section

Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA724468, 1/50, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature.

☑ Relative expression (RE)

This data was developed using HA724468, the same antibody clone in a different buffer formulation.
Application: Flow Cytometry (Intra)

Species: Human
Sample: Hep G2 (Human liver cancer cells)/HeLa (Human cervix adenocarcinoma epithelial cell)

Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.

Primary antibody: HA724468(1/200, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.

Negative expression of Albumin protein in HeLa cell is consistent with the predicted expression pattern.