MOG Recombinant Rabbit Monoclonal Antibody [JM23-06] - BSA and Azide free
Catalog# HA751833
MOG Recombinant Rabbit Monoclonal Antibody [JM23-06] - BSA and Azide free
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WB
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IHC-P
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IF-Tissue
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IHC-Fr
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Human
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Mouse
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Rat
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ET1705-16
含抗保成分
-
Cynomolgus monkey
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Pig
予測される交差反応性
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unconjugated
製品概要
製品名
MOG Recombinant Rabbit Monoclonal Antibody [JM23-06] - BSA and Azide free
抗体のタイプ
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human Myelin oligodendrocyte glycoprotein aa 51-100 / 247.
交差反応性（対応種属）
Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
予測される交差反応性
検証済みアプリケーション
WB, IHC-P, IF-Tissue, IHC-Fr
標的分子量
Predicted band size: 28 kDa
ポジティブコントロール
Mouse brain tissue lysate, rat brain tissue lysate, human cerebellum tissue, mouse cerebral cortex tissue, mouse hippocampus tissue, mouse brain tissue, rat brain tissue.
コンジュゲーション
unconjugated
クローン番号
JM23-06
製品の特徴
形態
Liquid
濃度
保存方法
Store at 2-8℃. Avoid freeze.
保存バッファー
1*PBS (pH7.4).
アイソタイプ
IgG
精製方法
Protein A affinity purified.
推奨希釈倍率
ターゲット
機能
Myelin oligodendrocyte glycoprotein (MOG) is a glycoprotein believed to be important in the myelination of nerves in the central nervous system (CNS). In humans this protein is encoded by the MOG gene. It is speculated to serve as a necessary "adhesion molecule" to provide structural integrity to the myelin sheath and is known to develop late on the oligodendrocyte. While the primary molecular function of MOG is not yet known, its likely role with the myelin sheath is either in sheath "completion and/or maintenance". More specifically, MOG is speculated to be "necessary" as an "adhesion molecule" on the myelin sheath of the CNS to provide the structural integrity of the myelin sheath."
背景・参考文献
1. Hoban AE et al. Regulation of prefrontal cortex myelination by the microbiota. Transl Psychiatry 6:e774 (2016).
2. Michailidou I et al. Complement C3 on microglial clusters in multiple sclerosis occur in chronic but not acute disease: Implication for disease pathogenesis. Glia 65(2):264-277 (2017).
配列相同性
Belongs to the immunoglobulin superfamily. BTN/MOG family.
組織特異性
Found exclusively in the CNS, where it is localized on the surface of myelin and oligodendrocyte cytoplasmic membranes.
サブセルラー局在
Cell membrane.
別名
BTN6 antibody
BTNL11 antibody
MGC26137 antibody
MOG alpha 5 antibody
MOG alpha 6 antibody
MOG AluA antibody
MOG AluB antibody
MOG antibody
MOG Ig AluB antibody
MOG_HUMAN antibody
詳細を見る画像
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☑ Relative expression (RE)
This data was developed using ET1705-16, the same antibody clone in a different buffer formulation.
Western blot analysis of MOG on different lysates with Rabbit anti-MOG antibody (ET1705-16) at 1/1,000 dilution.
Lane 1: Mouse brain tissue lysate (40 µg/Lane)
Lane 2: Mouse lung tissue lysate (negative) (40 µg/Lane)
Lane 3: Rat brain tissue lysate (40 µg/Lane)
Lane 4: Rat lung tissue lysate (negative) (40 µg/Lane)
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 1 minute; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-16) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using ET1705-16, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Mouse
Site: Cerebellum
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Recommend. The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature. -
This data was developed using ET1705-16, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-MOG antibody (ET1705-16) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-16) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1705-16, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-MOG antibody (ET1705-16) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-16) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1705-16, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-MOG antibody (ET1705-16) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-16) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1705-16, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-MOG antibody (ET1705-16) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-16) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1705-16, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-MOG antibody (ET1705-16) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-16) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1705-16, the same antibody clone in a different buffer formulation.
Application: IF-tissue
Species: Rat
Site: Cerebral cortex
Sample: Paraffin-embedded section
Antibody concentration: 1:200
ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）