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Presenilin 1 / PS-1 Recombinant Rabbit Monoclonal Antibody [PSH11-19]

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Catalog# HA723301

Presenilin 1 / PS-1 Recombinant Rabbit Monoclonal Antibody [PSH11-19]

Application

  • WB

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

概要

製品名

Presenilin 1 / PS-1 Recombinant Rabbit Monoclonal Antibody [PSH11-19]

抗体の種類

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within human Presenilin 1 aa 311-360.

製品の特異性

Presenilin 1 / PS-1 [PSH11-19] Rabbit mAb detects endogenous levels of the CTF (carboxy-terminal fragment) of presenilin 1 (22 kDa).

種属反応性

Human, Mouse, Rat

検証された応用例

WB, IP

分子量

Predicted band size: 53 kDa

陽性対照

HeLa cell lysate, HEK-293 cell lysate, MEF cell lysate, HepG2 cell lysate, HL-60 cell lysate, Daudi cell lysate, Neuro-2a cell lysate, Mouse brain tissue lysate, Mouse cerebellum tissue lysate, Rat brain tissue lysate, Rat cerebellum tissue lysate.

結合

unconjugated

クローン番号

PSH11-19

製品の特徴

形態

Liquid

保存方法

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

保存用バッファー

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

アイソタイプ

IgG

精製方法

Protein A affinity purified.

応用希釈度

  • WB

  • 1:2,000

  • IP

  • 1-2μg/sample

論文における応用例

WB 確認する 1 以下の論文

論文における種属

Human 確認する 1 以下の論文

ターゲット

機能

Presenilin-1 (PS-1) is a presenilin protein that in humans is encoded by the PSEN1 gene. Presenilin-1 is one of the four core proteins in the gamma secretase complex, which is considered to play an important role in generation of amyloid beta (Aβ) from amyloid-beta precursor protein (APP). Accumulation of amyloid beta is associated with the onset of Alzheimer's disease. Presenilins are postulated to regulate APP processing through their effects on gamma secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor, such that they either directly regulate gamma secretase activity or themselves are protease enzymes. Multiple alternatively spliced transcript variants have been identified for this gene, the full-length natures of only some have been determined.

背景文献

1. Yang Y et al. Presenilin-1 (PSEN1) Mutations: Clinical Phenotypes beyond Alzheimer\'s Disease. Int J Mol Sci. 2023 May

2. Gamez-Belmonte R et al. Epithelial presenilin-1 drives colorectal tumour growth by controlling EGFR-COX2 signalling. Gut. 2023 Jun

亜細胞局在

Endoplasmic reticulum, Endoplasmic reticulum membrane, Golgi apparatus membrane, Cytoplasmic granule, Cell membrane, Cell projection, growth cone, Early endosome, Early endosome membrane, neuron projection, axon, Synapse.

UNIPROT #

SwissProt: P49768 Human

SwissProt: P49769 Mouse

SwissProt: P97887 Rat

別名

AD3 antibody

Ad3h antibody

FAD antibody

Homo Sapiens Clone CC44 Senilin 1 antibody

Presenilin-1 CTF12 antibody

Protein S182 antibody

PS 1 antibody

PS-1 antibody

PS1-CTF12 antibody

PSEN1 antibody

展開

画像

  • Western blot analysis of Presenilin 1 / PS-1 on different lysates with Rabbit anti-Presenilin 1 / PS-1 antibody (<a href="/products/HA723301" style="font-weight: bold;text-decoration: underline;">HA723301</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: HEK-293 cell lysate (20 µg/Lane)<br />Lane 3: MEF cell lysate (20 µg/Lane)<br />Lane 4: HepG2 cell lysate (20 µg/Lane)<br />Lane 5: HL-60 cell lysate (20 µg/Lane)<br />Lane 6: Daudi cell lysate (20 µg/Lane)<br />Lane 7: Neuro-2a cell lysate (20 µg/Lane)<br />Lane 8: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 9: Mouse cerebellum tissue lysate (40 µg/Lane)<br />Lane 10: Rat brain tissue lysate (40 µg/Lane)<br />Lane 11: Rat cerebellum tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 53 kDa<br />Observed band size: 18 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723301" style="font-weight: bold;text-decoration: underline;">HA723301</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Presenilin 1 / PS-1 on different lysates with Rabbit anti-Presenilin 1 / PS-1 antibody (HA723301) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: HEK-293 cell lysate (20 µg/Lane)
    Lane 3: MEF cell lysate (20 µg/Lane)
    Lane 4: HepG2 cell lysate (20 µg/Lane)
    Lane 5: HL-60 cell lysate (20 µg/Lane)
    Lane 6: Daudi cell lysate (20 µg/Lane)
    Lane 7: Neuro-2a cell lysate (20 µg/Lane)
    Lane 8: Mouse brain tissue lysate (40 µg/Lane)
    Lane 9: Mouse cerebellum tissue lysate (40 µg/Lane)
    Lane 10: Rat brain tissue lysate (40 µg/Lane)
    Lane 11: Rat cerebellum tissue lysate (40 µg/Lane)

    Predicted band size: 53 kDa
    Observed band size: 18 kDa

    Exposure time: 25 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723301) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Presenilin 1 / PS-1 was immunoprecipitated from 0.2 mg HeLa cell lysate with <a href="/products/HA723301" style="font-weight: bold;text-decoration: underline;">HA723301</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA723301" style="font-weight: bold;text-decoration: underline;">HA723301</a> at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: HeLa cell lysate (input)<br />Lane 2: <a href="/products/HA723301" style="font-weight: bold;text-decoration: underline;">HA723301</a> IP in HeLa cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA723301" style="font-weight: bold;text-decoration: underline;">HA723301</a> in HeLa cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 10 seconds; ECL: K1801

    Presenilin 1 / PS-1 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA723301 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723301 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: HeLa cell lysate (input)
    Lane 2: HA723301 IP in HeLa cell lysate
    Lane 3: Rabbit IgG instead of HA723301 in HeLa cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 10 seconds; ECL: K1801

ご注意ください: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

論文での実績

Filter:
  1. WB

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