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F4/80 Recombinant Rabbit Monoclonal Antibody [PSH0-85]

RMB: 1500

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Catalog# HA721520

F4/80 Recombinant Rabbit Monoclonal Antibody [PSH0-85]

Application

  • WB

  • IHC-P

  • IF-Tissue

  • IHC-Fr

  • mIHC

Reactivity

  • Mouse

  • Rat

This product has been cited in peer reviewed publications, see list HERE

概要

製品名

F4/80 Recombinant Rabbit Monoclonal Antibody [PSH0-85]

抗体の種類

Recombinant Rabbit monoclonal Antibody

免疫原

Recombinant protein within mouse F4/80 aa 1-650 / 931.

種属反応性

Mouse, Rat

検証された応用例

WB, IHC-P, IF-Tissue, IHC-Fr, mIHC

分子量

Predicted band size: 102 kDa

陽性対照

RAW264.7 cell lysate, Rat spleen tissue lysate, mouse liver tissue, mouse spleen tissue, rat liver tissue, rat spleen tissue, mouse lung tissue, rat lung tissue.

結合

unconjugated

クローン番号

PSH0-85

RRID

AB_3072636

製品の特徴

形態

Liquid

濃度

1ug/ul

保存方法

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

保存用バッファー

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

アイソタイプ

IgG

精製方法

Protein A affinity purified.

応用希釈度

  • WB

  • 1:1,000

  • IHC-P

  • 1:200-1:1,000

  • IF-Tissue

  • 1:500

  • IHC-Fr

  • 1:1,000

  • mIHC

  • 1:500

ターゲット

機能

EGF-like module-containing mucin-like hormone receptor-like 1 also known as F4/80 is a protein encoded by the ADGRE1 gene. EMR1 is a member of the adhesion GPCR family. Adhesion GPCRs are characterized by an extended extracellular region often possessing N-terminal protein modules that is linked to a TM7 region via a domain known as the GPCR-Autoproteolysis INducing (GAIN) domain. EMR1 expression in human is restricted to eosinophils and is a specific marker for these cells. The murine homolog of EMR1, F4/80, is a well-known and widely used marker of murine macrophage populations. The N-terminal fragment (NTF) of EMR1 contains 4-6 Epidermal Growth Factor-like (EGF-like) domains in human and 4-7 EGF-like domains in the mouse. Utilizing F4/80 knockout mice, Lin et al. showed that F4/80 is not necessary for the development of tissue macrophages but is required for the induction of efferent CD8+ regulatory T cells needed for peripheral tolerance.

背景文献

1. Deng R et al. Periosteal CD68+ F4/80+ Macrophages Are Mechanosensitive for Cortical Bone Formation by Secretion and Activation of TGF-β1. Adv Sci (Weinh). 2022 Jan

2. Shin AE et al. F4/80+Ly6Chigh Macrophages Lead to Cell Plasticity and Cancer Initiation in Colitis. Gastroenterology. 2023 Apr

亜細胞局在

Cell membrane.

UNIPROT #

SwissProt: Q61549 Mouse

SwissProt: Q5Y4N8 Rat

別名

ADGRE1 antibody

Adhesion G protein coupled receptor E1 antibody

Adhesion G protein-coupled receptor E1 antibody

AGRE1_HUMAN antibody

Cell surface glycoprotein EMR1 antibody

Cell surface glycoprotein F4/80 antibody

DD7A5 7 antibody

Egf like module containing mucin like hormone receptor like 1 antibody

Egf like module containing mucin like hormone receptor like sequence 1 antibody

EGF like module receptor 1 antibody

展開

画像

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of F4/80 on different lysates with Rabbit anti-F4/80 antibody (<a href="/products/HA721520" style="font-weight: bold;text-decoration: underline;">HA721520</a>) at 1/1,000 dilution.<br /><br />Lane 1: RAW264.7 cell lysate (no heat) (20 µg/Lane)<br />Lane 2: L929 cell lysate (no heat) (negative) (20 µg/Lane)<br />Lane 3: Rat spleen tissue lysate (70℃ heat) (40 µg/Lane)<br /><br />Notice: no heat means the lysate is not boiled.<br /><br />Predicted band size: 102 kDa<br />Observed band size: 160 kDa<br /><br />Exposure time: 2 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721520" style="font-weight: bold;text-decoration: underline;">HA721520</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of F4/80 on different lysates with Rabbit anti-F4/80 antibody (HA721520) at 1/1,000 dilution.

    Lane 1: RAW264.7 cell lysate (no heat) (20 µg/Lane)
    Lane 2: L929 cell lysate (no heat) (negative) (20 µg/Lane)
    Lane 3: Rat spleen tissue lysate (70℃ heat) (40 µg/Lane)

    Notice: no heat means the lysate is not boiled.

    Predicted band size: 102 kDa
    Observed band size: 160 kDa

    Exposure time: 2 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721520) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Spleen<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/1,000 (F4/80, <a href="/products/HA721520" style="font-weight: bold;text-decoration: underline;">HA721520</a>, rabbit, red); 1/500 (Iba1, <a href="/products/HA601376" style="font-weight: bold;text-decoration: underline;">HA601376</a>, Chicken, green)<br /> <br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Spleen

    Sample: Frozen section

    Antibody concentration: 1/1,000 (F4/80, HA721520, rabbit, red); 1/500 (Iba1, HA601376, Chicken, green)

    Antigen retrieval: Not required

  • Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: Spleen<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/1,000 (F4/80, <a href="/products/HA721520" style="font-weight: bold;text-decoration: underline;">HA721520</a>, rabbit, red); 1/500 (Iba1, <a href="/products/HA601376" style="font-weight: bold;text-decoration: underline;">HA601376</a>, Chicken, green)<br /> <br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Rat

    Site: Spleen

    Sample: Frozen section

    Antibody concentration: 1/1,000 (F4/80, HA721520, rabbit, red); 1/500 (Iba1, HA601376, Chicken, green)

    Antigen retrieval: Not required

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-F4/80 antibody (<a href="/products/HA721520" style="font-weight: bold;text-decoration: underline;">HA721520</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721520" style="font-weight: bold;text-decoration: underline;">HA721520</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-F4/80 antibody (HA721520) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721520) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-F4/80 antibody (<a href="/products/HA721520" style="font-weight: bold;text-decoration: underline;">HA721520</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721520" style="font-weight: bold;text-decoration: underline;">HA721520</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-F4/80 antibody (HA721520) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721520) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-F4/80 antibody (<a href="/products/HA721520" style="font-weight: bold;text-decoration: underline;">HA721520</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721520" style="font-weight: bold;text-decoration: underline;">HA721520</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-F4/80 antibody (HA721520) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721520) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-F4/80 antibody (<a href="/products/HA721520" style="font-weight: bold;text-decoration: underline;">HA721520</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721520" style="font-weight: bold;text-decoration: underline;">HA721520</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-F4/80 antibody (HA721520) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721520) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-F4/80 antibody (<a href="/products/HA721520" style="font-weight: bold;text-decoration: underline;">HA721520</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721520" style="font-weight: bold;text-decoration: underline;">HA721520</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-F4/80 antibody (HA721520) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721520) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-F4/80 antibody (<a href="/products/HA721520" style="font-weight: bold;text-decoration: underline;">HA721520</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721520" style="font-weight: bold;text-decoration: underline;">HA721520</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-F4/80 antibody (HA721520) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721520) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: IF-tissue<br /><br />Species: Mouse<br /><br />Site: Liver<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/500

    Application: IF-tissue

    Species: Mouse

    Site: Liver

    Sample: Paraffin-embedded section

    Antibody concentration: 1/500

  • Application: IF-tissue<br /><br />Species: Rat<br /><br />Site: Liver<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/500

    Application: IF-tissue

    Species: Rat

    Site: Liver

    Sample: Paraffin-embedded section

    Antibody concentration: 1/500

  • mIHC analysis of mouse spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-F4/80 antibody (<a href="/products/HA721520" style="font-weight: bold;text-decoration: underline;">HA721520</a>) at 1/500 dilution. The immunostaining was performed with the IRISKit&reg; HyperView mTSA Kit (<a href="/products/MH900206" style="font-weight: bold;text-decoration: underline;">MH900206</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of mouse spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-F4/80 antibody (HA721520) at 1/500 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

ご注意ください: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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