GABA Transporter 1 / GAT 1 Recombinant Rabbit Monoclonal Antibody [JE34-61]
Catalog# HA721496
GABA Transporter 1 / GAT 1 Recombinant Rabbit Monoclonal Antibody [JE34-61]
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WB
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IHC-P
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IHC-Fr
-
IF-Tissue
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Human
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Mouse
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Rat
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HA751824
不含抗保成分
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Cynomolgus monkey
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Pig
予測される交差反応性
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unconjugated
製品概要
製品名
GABA Transporter 1 / GAT 1 Recombinant Rabbit Monoclonal Antibody [JE34-61]
抗体のタイプ
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human GABA Transporter 1/ GAT 1 aa 1-52.
交差反応性（対応種属）
Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
予測される交差反応性
検証済みアプリケーション
WB, IHC-P, IHC-Fr, IF-Tissue
標的分子量
Predicted band size: 67 kDa
ポジティブコントロール
Mouse cerebellum tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, human brain tissue, human liver tissue, mouse cerebellum tissue, mouse retina tissue, rat brain tissue, rat retina tissue.
コンジュゲーション
unconjugated
クローン番号
JE34-61
RRID
製品の特徴
形態
Liquid
濃度
保存方法
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
保存バッファー
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
アイソタイプ
IgG
精製方法
Protein A affinity purified.
推奨希釈倍率
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WB
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1:1,000
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IHC-P
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1:500-1:2,000
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IHC-Fr
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1:1,000
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IF-Tissue
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1:500
ターゲット
機能
GABA transporter 1 (GAT1) also known as sodium- and chloride-dependent GABA transporter 1 is a protein that in humans is encoded by the SLC6A1 gene and belongs to the solute carrier 6 (SLC6) family of transporters. It mediates gamma-aminobutyric acid's translocation from the extracellular to intracellular spaces within brain tissue and the central nervous system as a whole. GAT1 is a gamma-aminobutyric acid (GABA) transporter, which removes GABA from the synaptic cleft by shuttling it to presynaptic neurons (where GABA can be recycled) and astrocytes (where GABA can be broken down). GABA Transporter 1 uses energy from the dissipation of a Na+ gradient, aided by the presence of a Cl− gradient, to translocate GABA across CNS neuronal membranes. The stoichiometry for GABA Transporter 1 is 2 Na+: 1 Cl−: 1 GABA. The presence of a Cl−/Cl− exchange is also proposed because the Cl− transported across the membrane does not affect the net charge. GABA is also the primary inhibitory neurotransmitter in the cerebral cortex and has the highest level of expression within it. The GABA affinity (Km) of the mouse isoform of GAT1 is 8 μM. In the brain of a mature mammal, glutamate is converted to GABA by the enzyme glutamate decarboxylase (GAD) along with the addition of vitamin B6. GABA is then packed and released into the post-synaptic terminals of neurons after synthesis. GABA can also be used to form succinate, which is involved in the citric acid cycle. Vesicle uptake has been shown to prioritize newly synthesized GABA over preformed GABA, though the reasoning behind this mechanism is currently not completely understood. The regulation of the modular functioning of GATs is highly dependent on a multitude of second messengers and synaptic proteins.
背景・参考文献
1. Fattorini G et al. A Reappraisal of GAT-1 Localization in Neocortex. Front Cell Neurosci. 2020 Feb
2. Fattorini G et al. Microglial expression of GAT-1 in the cerebral cortex. Glia. 2020 Mar
サブセルラー局在
Cell membrane, Presynapse.
別名
GABATHG antibody
GABATR antibody
GABT 1 antibody
GABT1 antibody
GAT-1 antibody
GAT1 antibody
SC6A1_HUMAN antibody
Slc6a1 antibody
Sodium and chloride dependent GABA transporter 1 antibody
Sodium- and chloride-dependent GABA transporter 1 antibody
詳細を見る画像
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Application: IHC-Fr
Species: Mouse
Site: Cerebellum
Sample: Frozen section
Antibody concentration: 1:1,000
Antigen retrieval: Not required -
Western blot analysis of GABA Transporter 1 / GAT 1 on different lysates with Rabbit anti-GABA Transporter 1 / GAT 1 antibody (HA721496) at 1/1,000 dilution.
Lane 1: Mouse cerebellum tissue lysate
Lane 2: Mouse cerebellum tissue lysate (70℃ heat)
Lane 3: Mouse brain tissue lysate
Lane 4: Mouse brain tissue lysate (70℃ heat)
Lane 5: Rat brain tissue lysate
Lane 6: Rat brain tissue lysate (70℃ heat)
Lysates/proteins at 30 µg/Lane.
Predicted band size: 67 kDa
Observed band size: 67 kDa
Exposure time: Lane 1-4: 1 minute 40 seconds; Lane 5-6: 5 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721496) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-GABA Transporter 1 / GAT 1 antibody (HA721496) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721496) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-GABA Transporter 1 / GAT 1 antibody (HA721496) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721496) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse retina tissue with Rabbit anti-GABA Transporter 1 / GAT 1 antibody (HA721496) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721496) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-GABA Transporter 1 / GAT 1 antibody (HA721496) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721496) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-GABA Transporter 1 / GAT 1 antibody (HA721496) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721496) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat retina tissue with Rabbit anti-GABA Transporter 1 / GAT 1 antibody (HA721496) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721496) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-GABA Transporter 1 / GAT 1 antibody (HA721496) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721496) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-GABA Transporter 1 / GAT 1 antibody (HA721496) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721496) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Application: IF-tissue
Species: Mouse
Site: Cerebellum
Sample: Paraffin-embedded section
Antibody concentration: 1:500 -
Application: IF-tissue
Species: Rat
Site: Cerebellum
Sample: Paraffin-embedded section
Antibody concentration: 1:500 -
Application: IF-tissue
Species: Human
Site: Cerebellum
Sample: Paraffin-embedded section
Antibody concentration: 1:500
ご注意: 本製品はすべて「研究用試薬」です。人や動物の診断・治療目的、または臨床診断には使用できません。（FOR RESEARCH USE ONLY）